Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_937 and Pf6N2E2_938 are separated by 208 nucleotides Pf6N2E2_938 and Pf6N2E2_939 are separated by 411 nucleotides
Pf6N2E2_937 - Short-chain dehydrodenase (gene dltE), at 1,104,460 to 1,105,221
_937
Pf6N2E2_938 - hypothetical protein, at 1,105,430 to 1,106,008
_938
Pf6N2E2_939 - hypothetical protein, at 1,106,420 to 1,106,953
_939
Position (kb)
1105
1106
1107 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1104.503 kb on + strand at 1104.504 kb on - strand at 1104.504 kb on - strand at 1104.537 kb on + strand, within Pf6N2E2_937 at 1104.537 kb on + strand, within Pf6N2E2_937 at 1104.538 kb on - strand, within Pf6N2E2_937 at 1104.653 kb on + strand, within Pf6N2E2_937 at 1104.827 kb on + strand, within Pf6N2E2_937 at 1104.827 kb on + strand, within Pf6N2E2_937 at 1104.827 kb on + strand, within Pf6N2E2_937 at 1104.828 kb on - strand at 1104.908 kb on + strand, within Pf6N2E2_937 at 1104.999 kb on + strand, within Pf6N2E2_937 at 1104.999 kb on + strand, within Pf6N2E2_937 at 1104.999 kb on + strand, within Pf6N2E2_937 at 1104.999 kb on + strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.066 kb on + strand, within Pf6N2E2_937 at 1105.066 kb on + strand, within Pf6N2E2_937 at 1105.066 kb on + strand, within Pf6N2E2_937 at 1105.067 kb on - strand, within Pf6N2E2_937 at 1105.067 kb on - strand, within Pf6N2E2_937 at 1105.122 kb on + strand, within Pf6N2E2_937 at 1105.127 kb on - strand, within Pf6N2E2_937 at 1105.199 kb on - strand at 1105.304 kb on + strand at 1105.305 kb on - strand at 1105.305 kb on - strand at 1105.328 kb on - strand at 1105.328 kb on - strand at 1105.453 kb on + strand at 1105.472 kb on - strand at 1105.594 kb on - strand, within Pf6N2E2_938 at 1105.594 kb on - strand, within Pf6N2E2_938 at 1105.594 kb on - strand, within Pf6N2E2_938 at 1105.594 kb on - strand, within Pf6N2E2_938 at 1105.594 kb on - strand, within Pf6N2E2_938 at 1105.612 kb on + strand, within Pf6N2E2_938 at 1105.612 kb on + strand, within Pf6N2E2_938 at 1105.613 kb on - strand, within Pf6N2E2_938 at 1105.613 kb on - strand, within Pf6N2E2_938 at 1105.613 kb on - strand, within Pf6N2E2_938 at 1105.613 kb on - strand, within Pf6N2E2_938 at 1105.613 kb on - strand, within Pf6N2E2_938 at 1105.615 kb on - strand, within Pf6N2E2_938 at 1105.615 kb on - strand, within Pf6N2E2_938 at 1105.638 kb on + strand, within Pf6N2E2_938 at 1105.646 kb on - strand, within Pf6N2E2_938 at 1105.646 kb on - strand at 1105.646 kb on - strand, within Pf6N2E2_938 at 1105.719 kb on + strand, within Pf6N2E2_938 at 1105.720 kb on - strand, within Pf6N2E2_938 at 1105.720 kb on - strand, within Pf6N2E2_938 at 1106.067 kb on + strand at 1106.067 kb on + strand at 1106.068 kb on - strand at 1106.068 kb on - strand at 1106.095 kb on - strand at 1106.095 kb on - strand at 1106.095 kb on - strand at 1106.192 kb on - strand at 1106.257 kb on + strand at 1106.257 kb on + strand at 1106.257 kb on + strand at 1106.259 kb on + strand at 1106.259 kb on + strand at 1106.295 kb on + strand at 1106.295 kb on + strand at 1106.299 kb on + strand at 1106.299 kb on + strand at 1106.299 kb on + strand at 1106.299 kb on + strand at 1106.299 kb on + strand at 1106.299 kb on + strand at 1106.300 kb on - strand at 1106.372 kb on + strand at 1106.372 kb on + strand at 1106.524 kb on + strand, within Pf6N2E2_939 at 1106.525 kb on - strand, within Pf6N2E2_939 at 1106.592 kb on + strand, within Pf6N2E2_939 at 1106.593 kb on - strand, within Pf6N2E2_939 at 1106.673 kb on + strand, within Pf6N2E2_939 at 1106.674 kb on - strand, within Pf6N2E2_939
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 1,104,503 + -0.5 1,104,504 - -0.5 1,104,504 - -0.8 1,104,537 + Pf6N2E2_937 0.10 -0.3 1,104,537 + Pf6N2E2_937 0.10 -0.5 1,104,538 - Pf6N2E2_937 0.10 -0.5 1,104,653 + Pf6N2E2_937 0.25 +0.3 1,104,827 + Pf6N2E2_937 0.48 -0.5 1,104,827 + Pf6N2E2_937 0.48 -0.5 1,104,827 + Pf6N2E2_937 0.48 -0.1 1,104,828 - +0.5 1,104,908 + Pf6N2E2_937 0.59 -0.5 1,104,999 + Pf6N2E2_937 0.71 +0.7 1,104,999 + Pf6N2E2_937 0.71 +1.3 1,104,999 + Pf6N2E2_937 0.71 +0.1 1,104,999 + Pf6N2E2_937 0.71 -0.5 1,105,000 - Pf6N2E2_937 0.71 +0.8 1,105,000 - Pf6N2E2_937 0.71 -0.3 1,105,000 - Pf6N2E2_937 0.71 +1.1 1,105,000 - Pf6N2E2_937 0.71 +0.9 1,105,000 - Pf6N2E2_937 0.71 -0.5 1,105,000 - Pf6N2E2_937 0.71 -1.3 1,105,000 - Pf6N2E2_937 0.71 -0.5 1,105,000 - Pf6N2E2_937 0.71 -0.2 1,105,000 - Pf6N2E2_937 0.71 +1.3 1,105,066 + Pf6N2E2_937 0.80 +0.5 1,105,066 + Pf6N2E2_937 0.80 +1.7 1,105,066 + Pf6N2E2_937 0.80 +1.3 1,105,067 - Pf6N2E2_937 0.80 +1.1 1,105,067 - Pf6N2E2_937 0.80 +0.1 1,105,122 + Pf6N2E2_937 0.87 +0.3 1,105,127 - Pf6N2E2_937 0.88 +0.9 1,105,199 - +1.5 1,105,304 + -0.0 1,105,305 - -1.5 1,105,305 - -0.5 1,105,328 - -0.5 1,105,328 - -0.5 1,105,453 + -0.5 1,105,472 - -0.5 1,105,594 - Pf6N2E2_938 0.28 -0.5 1,105,594 - Pf6N2E2_938 0.28 -0.8 1,105,594 - Pf6N2E2_938 0.28 +0.5 1,105,594 - Pf6N2E2_938 0.28 -0.8 1,105,594 - Pf6N2E2_938 0.28 -1.2 1,105,612 + Pf6N2E2_938 0.31 +0.6 1,105,612 + Pf6N2E2_938 0.31 -0.5 1,105,613 - Pf6N2E2_938 0.32 -0.3 1,105,613 - Pf6N2E2_938 0.32 -1.5 1,105,613 - Pf6N2E2_938 0.32 -0.3 1,105,613 - Pf6N2E2_938 0.32 -0.5 1,105,613 - Pf6N2E2_938 0.32 -2.2 1,105,615 - Pf6N2E2_938 0.32 +0.6 1,105,615 - Pf6N2E2_938 0.32 +1.8 1,105,638 + Pf6N2E2_938 0.36 -1.5 1,105,646 - Pf6N2E2_938 0.37 +0.2 1,105,646 - +0.1 1,105,646 - Pf6N2E2_938 0.37 -0.5 1,105,719 + Pf6N2E2_938 0.50 +0.7 1,105,720 - Pf6N2E2_938 0.50 -1.5 1,105,720 - Pf6N2E2_938 0.50 +0.2 1,106,067 + +0.6 1,106,067 + -1.4 1,106,068 - +0.5 1,106,068 - -0.5 1,106,095 - -0.5 1,106,095 - +0.5 1,106,095 - +0.5 1,106,192 - -0.5 1,106,257 + +1.0 1,106,257 + -0.5 1,106,257 + +0.3 1,106,259 + +0.6 1,106,259 + -0.0 1,106,295 + +1.2 1,106,295 + +0.3 1,106,299 + -0.1 1,106,299 + +0.3 1,106,299 + -0.5 1,106,299 + -1.5 1,106,299 + +0.1 1,106,299 + +0.8 1,106,300 - -0.5 1,106,372 + -0.1 1,106,372 + -1.5 1,106,524 + Pf6N2E2_939 0.19 +0.7 1,106,525 - Pf6N2E2_939 0.20 +0.1 1,106,592 + Pf6N2E2_939 0.32 -0.1 1,106,593 - Pf6N2E2_939 0.32 -0.5 1,106,673 + Pf6N2E2_939 0.47 -0.5 1,106,674 - Pf6N2E2_939 0.48 -1.5
Or see this region's nucleotide sequence