Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_840 and Pf6N2E2_841 are separated by 10 nucleotides Pf6N2E2_841 and Pf6N2E2_842 are separated by 365 nucleotides Pf6N2E2_842 and Pf6N2E2_843 overlap by 4 nucleotides
Pf6N2E2_840 - Alcohol dehydrogenase (EC 1.1.1.1), at 985,335 to 986,321
_840
Pf6N2E2_841 - hypothetical protein, at 986,332 to 986,493
_841
Pf6N2E2_842 - Haloacid dehalogenase, type II (EC 3.8.1.2), at 986,859 to 987,566
_842
Pf6N2E2_843 - Phosphoglycolate phosphatase, archaeal type (EC 3.1.3.18), at 987,563 to 989,320
_843
Position (kb) 986
987
988 Strain fitness (log2 ratio) -2
-1
0
1
2 at 985.882 kb on + strand, within Pf6N2E2_840 at 985.882 kb on + strand, within Pf6N2E2_840 at 985.882 kb on + strand, within Pf6N2E2_840 at 985.883 kb on - strand, within Pf6N2E2_840 at 986.459 kb on + strand, within Pf6N2E2_841 at 986.459 kb on + strand, within Pf6N2E2_841 at 986.460 kb on - strand, within Pf6N2E2_841 at 986.491 kb on + strand at 986.491 kb on + strand at 986.491 kb on + strand at 986.491 kb on + strand at 986.505 kb on + strand at 986.505 kb on + strand at 986.505 kb on + strand at 986.505 kb on + strand at 986.506 kb on - strand at 986.594 kb on + strand at 986.594 kb on + strand at 986.598 kb on + strand at 986.599 kb on - strand at 986.599 kb on - strand at 986.735 kb on + strand at 986.735 kb on + strand at 986.735 kb on + strand at 986.735 kb on + strand at 986.735 kb on + strand at 986.736 kb on - strand at 986.737 kb on + strand at 986.737 kb on + strand at 986.774 kb on - strand at 986.779 kb on + strand at 986.779 kb on + strand at 986.779 kb on + strand at 986.779 kb on + strand at 986.780 kb on - strand at 986.837 kb on + strand at 986.837 kb on + strand at 986.837 kb on + strand at 986.837 kb on + strand at 986.838 kb on - strand at 986.838 kb on - strand at 986.838 kb on - strand at 986.838 kb on - strand at 986.841 kb on + strand at 986.841 kb on + strand at 986.842 kb on - strand at 987.006 kb on + strand, within Pf6N2E2_842 at 987.006 kb on + strand, within Pf6N2E2_842 at 987.006 kb on + strand, within Pf6N2E2_842 at 987.006 kb on + strand, within Pf6N2E2_842 at 987.007 kb on - strand, within Pf6N2E2_842 at 987.102 kb on + strand, within Pf6N2E2_842 at 987.103 kb on - strand, within Pf6N2E2_842 at 987.222 kb on - strand, within Pf6N2E2_842 at 987.328 kb on - strand, within Pf6N2E2_842 at 987.348 kb on + strand, within Pf6N2E2_842 at 987.348 kb on + strand, within Pf6N2E2_842 at 987.349 kb on - strand, within Pf6N2E2_842 at 987.349 kb on - strand, within Pf6N2E2_842 at 987.456 kb on + strand, within Pf6N2E2_842 at 987.456 kb on + strand, within Pf6N2E2_842 at 987.456 kb on + strand, within Pf6N2E2_842 at 987.457 kb on - strand, within Pf6N2E2_842 at 987.457 kb on - strand, within Pf6N2E2_842 at 987.457 kb on - strand, within Pf6N2E2_842 at 987.478 kb on - strand, within Pf6N2E2_842 at 987.557 kb on + strand at 987.557 kb on + strand at 987.557 kb on + strand at 987.558 kb on - strand at 987.558 kb on - strand at 987.558 kb on - strand at 987.558 kb on - strand at 987.558 kb on - strand at 987.558 kb on - strand at 987.564 kb on + strand at 987.564 kb on + strand at 987.564 kb on + strand at 987.565 kb on - strand at 987.565 kb on - strand at 987.565 kb on - strand at 987.565 kb on - strand at 987.565 kb on - strand at 987.565 kb on - strand at 987.733 kb on + strand at 987.734 kb on - strand at 987.734 kb on - strand at 987.734 kb on - strand at 987.734 kb on - strand at 987.796 kb on + strand, within Pf6N2E2_843 at 987.796 kb on + strand, within Pf6N2E2_843 at 987.796 kb on + strand, within Pf6N2E2_843 at 987.796 kb on + strand, within Pf6N2E2_843 at 987.796 kb on + strand, within Pf6N2E2_843 at 987.796 kb on + strand, within Pf6N2E2_843 at 987.796 kb on + strand, within Pf6N2E2_843 at 987.796 kb on + strand, within Pf6N2E2_843 at 987.796 kb on + strand, within Pf6N2E2_843 at 987.797 kb on - strand, within Pf6N2E2_843 at 987.797 kb on - strand, within Pf6N2E2_843 at 987.797 kb on - strand, within Pf6N2E2_843 at 987.797 kb on - strand, within Pf6N2E2_843 at 987.797 kb on - strand, within Pf6N2E2_843 at 987.797 kb on - strand, within Pf6N2E2_843 at 987.797 kb on - strand, within Pf6N2E2_843 at 987.906 kb on - strand, within Pf6N2E2_843 at 987.906 kb on - strand, within Pf6N2E2_843 at 987.921 kb on + strand, within Pf6N2E2_843 at 987.921 kb on + strand, within Pf6N2E2_843 at 987.922 kb on - strand, within Pf6N2E2_843 at 987.922 kb on - strand, within Pf6N2E2_843 at 987.922 kb on - strand, within Pf6N2E2_843 at 987.922 kb on - strand, within Pf6N2E2_843 at 987.922 kb on - strand, within Pf6N2E2_843 at 987.922 kb on - strand, within Pf6N2E2_843 at 987.995 kb on + strand, within Pf6N2E2_843 at 987.995 kb on + strand, within Pf6N2E2_843 at 987.995 kb on + strand, within Pf6N2E2_843 at 987.996 kb on - strand, within Pf6N2E2_843 at 987.996 kb on - strand, within Pf6N2E2_843 at 987.996 kb on - strand, within Pf6N2E2_843 at 987.996 kb on - strand, within Pf6N2E2_843 at 987.997 kb on + strand, within Pf6N2E2_843 at 987.997 kb on + strand, within Pf6N2E2_843 at 987.998 kb on - strand, within Pf6N2E2_843 at 988.061 kb on + strand, within Pf6N2E2_843 at 988.061 kb on + strand, within Pf6N2E2_843 at 988.061 kb on + strand, within Pf6N2E2_843 at 988.061 kb on + strand, within Pf6N2E2_843 at 988.062 kb on - strand, within Pf6N2E2_843 at 988.062 kb on - strand, within Pf6N2E2_843 at 988.149 kb on - strand, within Pf6N2E2_843 at 988.233 kb on + strand, within Pf6N2E2_843 at 988.233 kb on + strand, within Pf6N2E2_843 at 988.233 kb on + strand, within Pf6N2E2_843 at 988.233 kb on + strand, within Pf6N2E2_843 at 988.234 kb on - strand, within Pf6N2E2_843 at 988.304 kb on + strand, within Pf6N2E2_843 at 988.305 kb on - strand, within Pf6N2E2_843 at 988.367 kb on - strand, within Pf6N2E2_843 at 988.379 kb on + strand, within Pf6N2E2_843 at 988.380 kb on - strand, within Pf6N2E2_843 at 988.458 kb on + strand, within Pf6N2E2_843
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 985,882 + Pf6N2E2_840 0.55 -0.2 985,882 + Pf6N2E2_840 0.55 +1.4 985,882 + Pf6N2E2_840 0.55 +1.1 985,883 - Pf6N2E2_840 0.56 +0.7 986,459 + Pf6N2E2_841 0.78 +0.3 986,459 + Pf6N2E2_841 0.78 -0.3 986,460 - Pf6N2E2_841 0.79 -0.1 986,491 + -0.2 986,491 + +1.3 986,491 + +0.9 986,491 + -2.5 986,505 + -0.5 986,505 + +1.5 986,505 + +0.1 986,505 + +1.0 986,506 - +0.4 986,594 + +0.3 986,594 + +0.5 986,598 + -0.5 986,599 - -0.5 986,599 - -0.3 986,735 + +1.1 986,735 + +0.9 986,735 + +0.3 986,735 + +2.5 986,735 + -0.4 986,736 - -0.6 986,737 + +0.0 986,737 + -0.3 986,774 - +0.5 986,779 + +0.5 986,779 + -0.5 986,779 + -0.5 986,779 + -0.1 986,780 - -0.5 986,837 + +0.8 986,837 + +0.4 986,837 + -0.5 986,837 + +0.6 986,838 - +0.1 986,838 - -0.5 986,838 - +0.5 986,838 - -0.5 986,841 + -0.3 986,841 + +0.3 986,842 - -0.3 987,006 + Pf6N2E2_842 0.21 +1.4 987,006 + Pf6N2E2_842 0.21 +0.3 987,006 + Pf6N2E2_842 0.21 +0.0 987,006 + Pf6N2E2_842 0.21 +0.1 987,007 - Pf6N2E2_842 0.21 +1.5 987,102 + Pf6N2E2_842 0.34 +1.2 987,103 - Pf6N2E2_842 0.34 -2.5 987,222 - Pf6N2E2_842 0.51 -1.5 987,328 - Pf6N2E2_842 0.66 +0.9 987,348 + Pf6N2E2_842 0.69 +0.6 987,348 + Pf6N2E2_842 0.69 -0.5 987,349 - Pf6N2E2_842 0.69 +1.8 987,349 - Pf6N2E2_842 0.69 -0.5 987,456 + Pf6N2E2_842 0.84 +0.7 987,456 + Pf6N2E2_842 0.84 +1.5 987,456 + Pf6N2E2_842 0.84 -0.1 987,457 - Pf6N2E2_842 0.84 +0.7 987,457 - Pf6N2E2_842 0.84 +1.1 987,457 - Pf6N2E2_842 0.84 -0.5 987,478 - Pf6N2E2_842 0.87 -1.5 987,557 + -0.9 987,557 + -0.1 987,557 + +0.4 987,558 - -1.0 987,558 - +0.5 987,558 - +0.1 987,558 - -1.1 987,558 - +1.0 987,558 - +0.1 987,564 + -2.5 987,564 + +1.3 987,564 + -0.5 987,565 - -1.1 987,565 - +0.8 987,565 - +0.1 987,565 - -0.1 987,565 - +0.9 987,565 - -0.5 987,733 + -1.5 987,734 - +0.1 987,734 - -0.5 987,734 - +0.2 987,734 - +0.5 987,796 + Pf6N2E2_843 0.13 +0.5 987,796 + Pf6N2E2_843 0.13 +0.8 987,796 + Pf6N2E2_843 0.13 +1.5 987,796 + Pf6N2E2_843 0.13 -0.0 987,796 + Pf6N2E2_843 0.13 +0.7 987,796 + Pf6N2E2_843 0.13 +0.2 987,796 + Pf6N2E2_843 0.13 -0.5 987,796 + Pf6N2E2_843 0.13 +0.2 987,796 + Pf6N2E2_843 0.13 +1.6 987,797 - Pf6N2E2_843 0.13 +1.4 987,797 - Pf6N2E2_843 0.13 -0.3 987,797 - Pf6N2E2_843 0.13 -0.9 987,797 - Pf6N2E2_843 0.13 -0.2 987,797 - Pf6N2E2_843 0.13 +1.5 987,797 - Pf6N2E2_843 0.13 -1.5 987,797 - Pf6N2E2_843 0.13 -1.5 987,906 - Pf6N2E2_843 0.20 -2.1 987,906 - Pf6N2E2_843 0.20 +0.5 987,921 + Pf6N2E2_843 0.20 +2.1 987,921 + Pf6N2E2_843 0.20 -0.3 987,922 - Pf6N2E2_843 0.20 +0.8 987,922 - Pf6N2E2_843 0.20 +2.2 987,922 - Pf6N2E2_843 0.20 -0.5 987,922 - Pf6N2E2_843 0.20 -0.3 987,922 - Pf6N2E2_843 0.20 -0.7 987,922 - Pf6N2E2_843 0.20 +0.5 987,995 + Pf6N2E2_843 0.25 +0.3 987,995 + Pf6N2E2_843 0.25 +0.1 987,995 + Pf6N2E2_843 0.25 -1.5 987,996 - Pf6N2E2_843 0.25 +1.7 987,996 - Pf6N2E2_843 0.25 -1.5 987,996 - Pf6N2E2_843 0.25 +1.1 987,996 - Pf6N2E2_843 0.25 -2.5 987,997 + Pf6N2E2_843 0.25 -0.5 987,997 + Pf6N2E2_843 0.25 -0.1 987,998 - Pf6N2E2_843 0.25 -0.5 988,061 + Pf6N2E2_843 0.28 -1.5 988,061 + Pf6N2E2_843 0.28 +0.2 988,061 + Pf6N2E2_843 0.28 -0.1 988,061 + Pf6N2E2_843 0.28 -1.1 988,062 - Pf6N2E2_843 0.28 -1.4 988,062 - Pf6N2E2_843 0.28 -1.3 988,149 - Pf6N2E2_843 0.33 -0.5 988,233 + Pf6N2E2_843 0.38 -1.5 988,233 + Pf6N2E2_843 0.38 +0.5 988,233 + Pf6N2E2_843 0.38 +0.5 988,233 + Pf6N2E2_843 0.38 -0.1 988,234 - Pf6N2E2_843 0.38 -0.5 988,304 + Pf6N2E2_843 0.42 -0.5 988,305 - Pf6N2E2_843 0.42 -0.1 988,367 - Pf6N2E2_843 0.46 -1.7 988,379 + Pf6N2E2_843 0.46 +0.4 988,380 - Pf6N2E2_843 0.46 -0.5 988,458 + Pf6N2E2_843 0.51 -0.5
Or see this region's nucleotide sequence