Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6033 and Pf6N2E2_6034 overlap by 4 nucleotides Pf6N2E2_6034 and Pf6N2E2_6035 are separated by 15 nucleotides Pf6N2E2_6035 and Pf6N2E2_6036 are separated by 97 nucleotides
Pf6N2E2_6033 - Sterol desaturase, at 6,849,429 to 6,850,526
_6033
Pf6N2E2_6034 - FIG00965471: hypothetical protein, at 6,850,523 to 6,851,338
_6034
Pf6N2E2_6035 - alpha/beta hydrolase fold, at 6,851,354 to 6,852,034
_6035
Pf6N2E2_6036 - hypothetical protein, at 6,852,132 to 6,852,755
_6036
Position (kb)
6850
6851
6852 Strain fitness (log2 ratio)
-1
0
1
2 at 6849.552 kb on + strand, within Pf6N2E2_6033 at 6849.553 kb on - strand, within Pf6N2E2_6033 at 6849.678 kb on + strand, within Pf6N2E2_6033 at 6849.678 kb on + strand, within Pf6N2E2_6033 at 6849.678 kb on + strand, within Pf6N2E2_6033 at 6849.678 kb on + strand, within Pf6N2E2_6033 at 6849.681 kb on + strand, within Pf6N2E2_6033 at 6849.681 kb on + strand, within Pf6N2E2_6033 at 6849.744 kb on + strand, within Pf6N2E2_6033 at 6849.744 kb on + strand, within Pf6N2E2_6033 at 6849.744 kb on + strand, within Pf6N2E2_6033 at 6849.744 kb on + strand, within Pf6N2E2_6033 at 6849.745 kb on - strand, within Pf6N2E2_6033 at 6849.745 kb on - strand, within Pf6N2E2_6033 at 6849.745 kb on - strand, within Pf6N2E2_6033 at 6849.766 kb on - strand, within Pf6N2E2_6033 at 6849.838 kb on - strand, within Pf6N2E2_6033 at 6849.877 kb on - strand, within Pf6N2E2_6033 at 6849.949 kb on + strand, within Pf6N2E2_6033 at 6850.047 kb on + strand, within Pf6N2E2_6033 at 6850.048 kb on - strand, within Pf6N2E2_6033 at 6850.048 kb on - strand, within Pf6N2E2_6033 at 6850.048 kb on - strand, within Pf6N2E2_6033 at 6850.095 kb on + strand, within Pf6N2E2_6033 at 6850.095 kb on + strand, within Pf6N2E2_6033 at 6850.096 kb on - strand, within Pf6N2E2_6033 at 6850.290 kb on + strand, within Pf6N2E2_6033 at 6850.290 kb on + strand, within Pf6N2E2_6033 at 6850.291 kb on - strand, within Pf6N2E2_6033 at 6850.291 kb on - strand, within Pf6N2E2_6033 at 6850.291 kb on - strand, within Pf6N2E2_6033 at 6850.365 kb on + strand, within Pf6N2E2_6033 at 6850.365 kb on + strand, within Pf6N2E2_6033 at 6850.365 kb on + strand, within Pf6N2E2_6033 at 6850.365 kb on + strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.366 kb on - strand, within Pf6N2E2_6033 at 6850.367 kb on + strand, within Pf6N2E2_6033 at 6850.408 kb on - strand, within Pf6N2E2_6033 at 6850.426 kb on - strand at 6850.718 kb on + strand, within Pf6N2E2_6034 at 6850.718 kb on + strand, within Pf6N2E2_6034 at 6850.843 kb on + strand, within Pf6N2E2_6034 at 6850.897 kb on + strand, within Pf6N2E2_6034 at 6850.897 kb on + strand, within Pf6N2E2_6034 at 6850.914 kb on - strand, within Pf6N2E2_6034 at 6850.914 kb on - strand, within Pf6N2E2_6034 at 6850.914 kb on - strand, within Pf6N2E2_6034 at 6851.209 kb on - strand, within Pf6N2E2_6034 at 6851.234 kb on + strand, within Pf6N2E2_6034 at 6851.235 kb on - strand, within Pf6N2E2_6034 at 6851.235 kb on - strand, within Pf6N2E2_6034 at 6851.305 kb on + strand at 6851.912 kb on + strand, within Pf6N2E2_6035 at 6851.913 kb on - strand, within Pf6N2E2_6035 at 6851.913 kb on - strand, within Pf6N2E2_6035 at 6851.980 kb on + strand at 6852.115 kb on - strand at 6852.271 kb on - strand, within Pf6N2E2_6036 at 6852.279 kb on + strand, within Pf6N2E2_6036 at 6852.279 kb on + strand, within Pf6N2E2_6036 at 6852.280 kb on - strand, within Pf6N2E2_6036 at 6852.280 kb on - strand, within Pf6N2E2_6036 at 6852.319 kb on + strand, within Pf6N2E2_6036 at 6852.320 kb on - strand, within Pf6N2E2_6036 at 6852.336 kb on + strand, within Pf6N2E2_6036 at 6852.336 kb on + strand, within Pf6N2E2_6036
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 6,849,552 + Pf6N2E2_6033 0.11 -1.1 6,849,553 - Pf6N2E2_6033 0.11 +0.9 6,849,678 + Pf6N2E2_6033 0.23 +0.9 6,849,678 + Pf6N2E2_6033 0.23 +0.9 6,849,678 + Pf6N2E2_6033 0.23 -0.7 6,849,678 + Pf6N2E2_6033 0.23 -0.1 6,849,681 + Pf6N2E2_6033 0.23 +0.8 6,849,681 + Pf6N2E2_6033 0.23 +0.1 6,849,744 + Pf6N2E2_6033 0.29 -0.1 6,849,744 + Pf6N2E2_6033 0.29 +0.2 6,849,744 + Pf6N2E2_6033 0.29 +0.1 6,849,744 + Pf6N2E2_6033 0.29 -1.1 6,849,745 - Pf6N2E2_6033 0.29 -1.3 6,849,745 - Pf6N2E2_6033 0.29 -0.1 6,849,745 - Pf6N2E2_6033 0.29 -1.4 6,849,766 - Pf6N2E2_6033 0.31 +0.2 6,849,838 - Pf6N2E2_6033 0.37 -0.6 6,849,877 - Pf6N2E2_6033 0.41 +0.5 6,849,949 + Pf6N2E2_6033 0.47 +1.1 6,850,047 + Pf6N2E2_6033 0.56 +0.9 6,850,048 - Pf6N2E2_6033 0.56 -0.1 6,850,048 - Pf6N2E2_6033 0.56 -0.1 6,850,048 - Pf6N2E2_6033 0.56 -0.1 6,850,095 + Pf6N2E2_6033 0.61 +0.9 6,850,095 + Pf6N2E2_6033 0.61 +0.3 6,850,096 - Pf6N2E2_6033 0.61 -0.1 6,850,290 + Pf6N2E2_6033 0.78 +2.1 6,850,290 + Pf6N2E2_6033 0.78 +0.2 6,850,291 - Pf6N2E2_6033 0.79 -0.6 6,850,291 - Pf6N2E2_6033 0.79 -0.1 6,850,291 - Pf6N2E2_6033 0.79 -1.1 6,850,365 + Pf6N2E2_6033 0.85 -0.1 6,850,365 + Pf6N2E2_6033 0.85 -0.7 6,850,365 + Pf6N2E2_6033 0.85 -1.1 6,850,365 + Pf6N2E2_6033 0.85 +1.5 6,850,366 - Pf6N2E2_6033 0.85 -0.1 6,850,366 - Pf6N2E2_6033 0.85 +0.3 6,850,366 - Pf6N2E2_6033 0.85 -0.2 6,850,366 - Pf6N2E2_6033 0.85 +0.5 6,850,366 - Pf6N2E2_6033 0.85 +0.5 6,850,366 - Pf6N2E2_6033 0.85 -0.3 6,850,366 - Pf6N2E2_6033 0.85 +0.5 6,850,366 - Pf6N2E2_6033 0.85 -0.3 6,850,367 + Pf6N2E2_6033 0.85 +1.2 6,850,408 - Pf6N2E2_6033 0.89 -0.1 6,850,426 - -0.1 6,850,718 + Pf6N2E2_6034 0.24 +0.2 6,850,718 + Pf6N2E2_6034 0.24 -0.6 6,850,843 + Pf6N2E2_6034 0.39 -0.1 6,850,897 + Pf6N2E2_6034 0.46 -0.4 6,850,897 + Pf6N2E2_6034 0.46 -0.1 6,850,914 - Pf6N2E2_6034 0.48 +0.3 6,850,914 - Pf6N2E2_6034 0.48 -0.5 6,850,914 - Pf6N2E2_6034 0.48 -0.5 6,851,209 - Pf6N2E2_6034 0.84 +0.8 6,851,234 + Pf6N2E2_6034 0.87 -0.8 6,851,235 - Pf6N2E2_6034 0.87 -0.9 6,851,235 - Pf6N2E2_6034 0.87 -0.6 6,851,305 + -0.1 6,851,912 + Pf6N2E2_6035 0.82 -0.6 6,851,913 - Pf6N2E2_6035 0.82 +0.4 6,851,913 - Pf6N2E2_6035 0.82 -0.6 6,851,980 + +0.9 6,852,115 - +0.5 6,852,271 - Pf6N2E2_6036 0.22 +0.4 6,852,279 + Pf6N2E2_6036 0.24 -0.1 6,852,279 + Pf6N2E2_6036 0.24 -1.1 6,852,280 - Pf6N2E2_6036 0.24 -1.1 6,852,280 - Pf6N2E2_6036 0.24 -0.5 6,852,319 + Pf6N2E2_6036 0.30 -0.1 6,852,320 - Pf6N2E2_6036 0.30 +0.8 6,852,336 + Pf6N2E2_6036 0.33 +0.1 6,852,336 + Pf6N2E2_6036 0.33 +0.2
Or see this region's nucleotide sequence