Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5933 and Pf6N2E2_5934 are separated by 23 nucleotides Pf6N2E2_5934 and Pf6N2E2_5935 overlap by 4 nucleotides Pf6N2E2_5935 and Pf6N2E2_5936 overlap by 1 nucleotides Pf6N2E2_5936 and Pf6N2E2_5937 overlap by 4 nucleotides
Pf6N2E2_5933 - Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130), at 6,753,413 to 6,754,423
_5933
Pf6N2E2_5934 - FIG002473: Protein YcaR in KDO2-Lipid A biosynthesis cluster, at 6,754,447 to 6,754,632
_5934
Pf6N2E2_5935 - 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38), at 6,754,629 to 6,755,393
_5935
Pf6N2E2_5936 - Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48), at 6,755,393 to 6,755,857
_5936
Pf6N2E2_5937 - UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158), at 6,755,854 to 6,756,873
_5937
Position (kb)
6755
6756 Strain fitness (log2 ratio)
-1
0
1
2
3 at 6754.421 kb on + strand at 6754.431 kb on + strand at 6755.422 kb on + strand at 6755.633 kb on + strand, within Pf6N2E2_5936 at 6755.765 kb on + strand, within Pf6N2E2_5936
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 6,754,421 + -0.2 6,754,431 + -0.1 6,755,422 + +0.0 6,755,633 + Pf6N2E2_5936 0.52 +1.3 6,755,765 + Pf6N2E2_5936 0.80 +2.9
Or see this region's nucleotide sequence