Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5395 and Pf6N2E2_5396 are separated by 128 nucleotides Pf6N2E2_5396 and Pf6N2E2_5397 are separated by 89 nucleotides
Pf6N2E2_5395 - Arginine/ornithine ABC transporter, periplasmic arginine/ornithine binding protein, at 6,200,942 to 6,201,676
_5395
Pf6N2E2_5396 - YheO-like PAS domain, at 6,201,805 to 6,202,431
_5396
Pf6N2E2_5397 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 6,202,521 to 6,203,477
_5397
Position (kb)
6201
6202
6203 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6200.832 kb on + strand at 6201.053 kb on + strand, within Pf6N2E2_5395 at 6201.053 kb on + strand, within Pf6N2E2_5395 at 6201.054 kb on - strand, within Pf6N2E2_5395 at 6201.054 kb on - strand, within Pf6N2E2_5395 at 6201.221 kb on + strand, within Pf6N2E2_5395 at 6201.221 kb on + strand, within Pf6N2E2_5395 at 6201.221 kb on + strand, within Pf6N2E2_5395 at 6201.222 kb on - strand, within Pf6N2E2_5395 at 6201.222 kb on - strand, within Pf6N2E2_5395 at 6201.222 kb on - strand, within Pf6N2E2_5395 at 6201.224 kb on + strand, within Pf6N2E2_5395 at 6201.224 kb on + strand, within Pf6N2E2_5395 at 6201.225 kb on - strand, within Pf6N2E2_5395 at 6201.225 kb on - strand, within Pf6N2E2_5395 at 6201.225 kb on - strand, within Pf6N2E2_5395 at 6201.225 kb on - strand, within Pf6N2E2_5395 at 6201.278 kb on + strand, within Pf6N2E2_5395 at 6201.278 kb on + strand, within Pf6N2E2_5395 at 6201.279 kb on - strand, within Pf6N2E2_5395 at 6201.279 kb on - strand, within Pf6N2E2_5395 at 6201.279 kb on - strand, within Pf6N2E2_5395 at 6201.380 kb on + strand, within Pf6N2E2_5395 at 6201.380 kb on + strand, within Pf6N2E2_5395 at 6201.381 kb on - strand, within Pf6N2E2_5395 at 6201.382 kb on + strand, within Pf6N2E2_5395 at 6201.382 kb on + strand, within Pf6N2E2_5395 at 6201.382 kb on + strand, within Pf6N2E2_5395 at 6201.382 kb on + strand, within Pf6N2E2_5395 at 6201.382 kb on + strand, within Pf6N2E2_5395 at 6201.382 kb on + strand, within Pf6N2E2_5395 at 6201.383 kb on - strand, within Pf6N2E2_5395 at 6201.383 kb on - strand, within Pf6N2E2_5395 at 6201.383 kb on - strand, within Pf6N2E2_5395 at 6201.383 kb on - strand, within Pf6N2E2_5395 at 6201.383 kb on - strand, within Pf6N2E2_5395 at 6201.383 kb on - strand, within Pf6N2E2_5395 at 6201.383 kb on - strand, within Pf6N2E2_5395 at 6201.383 kb on - strand, within Pf6N2E2_5395 at 6201.383 kb on - strand, within Pf6N2E2_5395 at 6201.401 kb on + strand, within Pf6N2E2_5395 at 6201.401 kb on + strand, within Pf6N2E2_5395 at 6201.401 kb on + strand, within Pf6N2E2_5395 at 6201.401 kb on + strand, within Pf6N2E2_5395 at 6201.401 kb on + strand, within Pf6N2E2_5395 at 6201.401 kb on + strand, within Pf6N2E2_5395 at 6201.491 kb on + strand, within Pf6N2E2_5395 at 6201.527 kb on + strand, within Pf6N2E2_5395 at 6201.528 kb on - strand, within Pf6N2E2_5395 at 6201.626 kb on + strand at 6201.626 kb on + strand at 6201.626 kb on + strand at 6201.626 kb on + strand at 6201.647 kb on + strand at 6201.647 kb on + strand at 6201.647 kb on + strand at 6201.647 kb on + strand at 6201.665 kb on + strand at 6201.665 kb on + strand at 6201.666 kb on - strand at 6201.677 kb on + strand at 6201.677 kb on + strand at 6201.678 kb on - strand at 6201.678 kb on - strand at 6201.728 kb on + strand at 6201.728 kb on + strand at 6201.728 kb on + strand at 6201.728 kb on + strand at 6201.728 kb on + strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.729 kb on - strand at 6201.746 kb on + strand at 6201.747 kb on - strand at 6201.833 kb on + strand at 6201.833 kb on + strand at 6201.833 kb on + strand at 6201.834 kb on - strand at 6201.834 kb on - strand at 6201.852 kb on + strand at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.872 kb on + strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6201.873 kb on - strand, within Pf6N2E2_5396 at 6202.110 kb on + strand, within Pf6N2E2_5396 at 6202.111 kb on - strand, within Pf6N2E2_5396 at 6202.162 kb on + strand, within Pf6N2E2_5396 at 6202.163 kb on - strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.224 kb on + strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.225 kb on - strand, within Pf6N2E2_5396 at 6202.297 kb on - strand, within Pf6N2E2_5396 at 6202.307 kb on + strand, within Pf6N2E2_5396 at 6202.307 kb on + strand, within Pf6N2E2_5396 at 6202.308 kb on - strand, within Pf6N2E2_5396 at 6202.308 kb on - strand, within Pf6N2E2_5396 at 6202.308 kb on - strand, within Pf6N2E2_5396 at 6202.308 kb on - strand, within Pf6N2E2_5396 at 6202.358 kb on + strand, within Pf6N2E2_5396 at 6202.358 kb on + strand, within Pf6N2E2_5396 at 6202.722 kb on + strand, within Pf6N2E2_5397 at 6202.723 kb on - strand, within Pf6N2E2_5397 at 6202.795 kb on + strand, within Pf6N2E2_5397 at 6202.796 kb on - strand, within Pf6N2E2_5397 at 6202.796 kb on - strand, within Pf6N2E2_5397 at 6202.796 kb on - strand, within Pf6N2E2_5397 at 6202.796 kb on - strand, within Pf6N2E2_5397 at 6202.830 kb on + strand, within Pf6N2E2_5397 at 6202.830 kb on + strand, within Pf6N2E2_5397 at 6202.830 kb on + strand, within Pf6N2E2_5397 at 6202.831 kb on - strand, within Pf6N2E2_5397 at 6202.831 kb on - strand, within Pf6N2E2_5397 at 6202.831 kb on - strand, within Pf6N2E2_5397 at 6202.831 kb on - strand, within Pf6N2E2_5397 at 6202.898 kb on + strand, within Pf6N2E2_5397 at 6202.898 kb on + strand, within Pf6N2E2_5397 at 6202.898 kb on + strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.899 kb on - strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.900 kb on + strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6202.901 kb on - strand, within Pf6N2E2_5397 at 6203.178 kb on - strand, within Pf6N2E2_5397 at 6203.178 kb on - strand, within Pf6N2E2_5397 at 6203.188 kb on + strand, within Pf6N2E2_5397 at 6203.188 kb on + strand, within Pf6N2E2_5397 at 6203.189 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.237 kb on - strand, within Pf6N2E2_5397 at 6203.342 kb on - strand, within Pf6N2E2_5397
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 6,200,832 + -0.1 6,201,053 + Pf6N2E2_5395 0.15 -0.8 6,201,053 + Pf6N2E2_5395 0.15 -0.4 6,201,054 - Pf6N2E2_5395 0.15 +0.2 6,201,054 - Pf6N2E2_5395 0.15 +1.2 6,201,221 + Pf6N2E2_5395 0.38 -0.8 6,201,221 + Pf6N2E2_5395 0.38 -2.6 6,201,221 + Pf6N2E2_5395 0.38 +1.5 6,201,222 - Pf6N2E2_5395 0.38 +0.8 6,201,222 - Pf6N2E2_5395 0.38 +0.2 6,201,222 - Pf6N2E2_5395 0.38 -0.6 6,201,224 + Pf6N2E2_5395 0.38 -0.8 6,201,224 + Pf6N2E2_5395 0.38 +0.0 6,201,225 - Pf6N2E2_5395 0.39 +0.6 6,201,225 - Pf6N2E2_5395 0.39 +0.8 6,201,225 - Pf6N2E2_5395 0.39 +0.2 6,201,225 - Pf6N2E2_5395 0.39 +0.2 6,201,278 + Pf6N2E2_5395 0.46 +0.6 6,201,278 + Pf6N2E2_5395 0.46 -0.5 6,201,279 - Pf6N2E2_5395 0.46 -0.8 6,201,279 - Pf6N2E2_5395 0.46 +0.3 6,201,279 - Pf6N2E2_5395 0.46 +0.2 6,201,380 + Pf6N2E2_5395 0.60 +1.2 6,201,380 + Pf6N2E2_5395 0.60 +0.3 6,201,381 - Pf6N2E2_5395 0.60 -0.5 6,201,382 + Pf6N2E2_5395 0.60 +0.2 6,201,382 + Pf6N2E2_5395 0.60 +0.1 6,201,382 + Pf6N2E2_5395 0.60 +1.0 6,201,382 + Pf6N2E2_5395 0.60 +0.4 6,201,382 + Pf6N2E2_5395 0.60 -0.4 6,201,382 + Pf6N2E2_5395 0.60 -0.1 6,201,383 - Pf6N2E2_5395 0.60 +1.2 6,201,383 - Pf6N2E2_5395 0.60 -0.1 6,201,383 - Pf6N2E2_5395 0.60 +0.4 6,201,383 - Pf6N2E2_5395 0.60 -0.8 6,201,383 - Pf6N2E2_5395 0.60 +0.2 6,201,383 - Pf6N2E2_5395 0.60 +0.2 6,201,383 - Pf6N2E2_5395 0.60 -0.3 6,201,383 - Pf6N2E2_5395 0.60 -0.8 6,201,383 - Pf6N2E2_5395 0.60 +0.2 6,201,401 + Pf6N2E2_5395 0.62 +0.4 6,201,401 + Pf6N2E2_5395 0.62 -0.8 6,201,401 + Pf6N2E2_5395 0.62 -0.3 6,201,401 + Pf6N2E2_5395 0.62 -0.4 6,201,401 + Pf6N2E2_5395 0.62 +0.6 6,201,401 + Pf6N2E2_5395 0.62 +0.2 6,201,491 + Pf6N2E2_5395 0.75 +0.4 6,201,527 + Pf6N2E2_5395 0.80 -0.0 6,201,528 - Pf6N2E2_5395 0.80 +1.7 6,201,626 + +0.9 6,201,626 + -1.1 6,201,626 + -0.1 6,201,626 + -1.6 6,201,647 + -0.3 6,201,647 + -0.1 6,201,647 + +0.5 6,201,647 + -0.8 6,201,665 + +1.5 6,201,665 + +0.5 6,201,666 - +1.4 6,201,677 + +0.5 6,201,677 + +0.0 6,201,678 - +0.5 6,201,678 - -0.4 6,201,728 + -0.4 6,201,728 + +1.2 6,201,728 + +1.0 6,201,728 + -0.1 6,201,728 + +0.2 6,201,729 - -0.5 6,201,729 - +0.7 6,201,729 - +0.3 6,201,729 - -0.6 6,201,729 - +1.4 6,201,729 - +0.1 6,201,729 - +0.8 6,201,729 - +0.5 6,201,729 - +0.2 6,201,729 - +0.7 6,201,746 + +0.6 6,201,747 - +1.3 6,201,833 + +1.5 6,201,833 + +0.2 6,201,833 + +1.2 6,201,834 - +1.6 6,201,834 - +0.2 6,201,852 + +1.2 6,201,872 + Pf6N2E2_5396 0.11 +0.2 6,201,872 + Pf6N2E2_5396 0.11 +1.3 6,201,872 + Pf6N2E2_5396 0.11 +0.0 6,201,872 + Pf6N2E2_5396 0.11 -0.4 6,201,872 + Pf6N2E2_5396 0.11 +1.5 6,201,872 + Pf6N2E2_5396 0.11 +0.6 6,201,873 - Pf6N2E2_5396 0.11 -0.3 6,201,873 - Pf6N2E2_5396 0.11 +0.8 6,201,873 - Pf6N2E2_5396 0.11 +0.6 6,201,873 - Pf6N2E2_5396 0.11 +0.2 6,201,873 - Pf6N2E2_5396 0.11 +1.0 6,201,873 - Pf6N2E2_5396 0.11 +1.7 6,201,873 - Pf6N2E2_5396 0.11 +0.9 6,201,873 - Pf6N2E2_5396 0.11 +0.7 6,201,873 - Pf6N2E2_5396 0.11 +2.2 6,201,873 - Pf6N2E2_5396 0.11 +1.1 6,201,873 - Pf6N2E2_5396 0.11 +1.2 6,201,873 - Pf6N2E2_5396 0.11 +1.6 6,202,110 + Pf6N2E2_5396 0.49 +1.5 6,202,111 - Pf6N2E2_5396 0.49 +0.3 6,202,162 + Pf6N2E2_5396 0.57 +0.2 6,202,163 - Pf6N2E2_5396 0.57 +0.2 6,202,224 + Pf6N2E2_5396 0.67 +0.2 6,202,224 + Pf6N2E2_5396 0.67 -0.4 6,202,224 + Pf6N2E2_5396 0.67 +0.6 6,202,224 + Pf6N2E2_5396 0.67 +1.2 6,202,224 + Pf6N2E2_5396 0.67 +1.2 6,202,224 + Pf6N2E2_5396 0.67 +0.7 6,202,224 + Pf6N2E2_5396 0.67 +0.7 6,202,224 + Pf6N2E2_5396 0.67 +1.4 6,202,224 + Pf6N2E2_5396 0.67 +2.5 6,202,225 - Pf6N2E2_5396 0.67 +1.0 6,202,225 - Pf6N2E2_5396 0.67 +1.5 6,202,225 - Pf6N2E2_5396 0.67 +0.5 6,202,225 - Pf6N2E2_5396 0.67 +2.8 6,202,225 - Pf6N2E2_5396 0.67 +1.2 6,202,225 - Pf6N2E2_5396 0.67 +1.4 6,202,225 - Pf6N2E2_5396 0.67 +1.2 6,202,297 - Pf6N2E2_5396 0.78 +0.2 6,202,307 + Pf6N2E2_5396 0.80 +1.1 6,202,307 + Pf6N2E2_5396 0.80 +0.2 6,202,308 - Pf6N2E2_5396 0.80 +1.6 6,202,308 - Pf6N2E2_5396 0.80 +1.4 6,202,308 - Pf6N2E2_5396 0.80 +0.6 6,202,308 - Pf6N2E2_5396 0.80 +0.2 6,202,358 + Pf6N2E2_5396 0.88 +1.3 6,202,358 + Pf6N2E2_5396 0.88 +0.2 6,202,722 + Pf6N2E2_5397 0.21 -0.8 6,202,723 - Pf6N2E2_5397 0.21 -1.2 6,202,795 + Pf6N2E2_5397 0.29 -1.8 6,202,796 - Pf6N2E2_5397 0.29 +0.2 6,202,796 - Pf6N2E2_5397 0.29 -1.1 6,202,796 - Pf6N2E2_5397 0.29 +0.2 6,202,796 - Pf6N2E2_5397 0.29 +0.7 6,202,830 + Pf6N2E2_5397 0.32 -0.2 6,202,830 + Pf6N2E2_5397 0.32 -0.8 6,202,830 + Pf6N2E2_5397 0.32 +1.2 6,202,831 - Pf6N2E2_5397 0.32 +0.9 6,202,831 - Pf6N2E2_5397 0.32 -0.6 6,202,831 - Pf6N2E2_5397 0.32 +0.7 6,202,831 - Pf6N2E2_5397 0.32 -0.3 6,202,898 + Pf6N2E2_5397 0.39 -0.6 6,202,898 + Pf6N2E2_5397 0.39 +0.7 6,202,898 + Pf6N2E2_5397 0.39 +0.0 6,202,899 - Pf6N2E2_5397 0.39 -0.7 6,202,899 - Pf6N2E2_5397 0.39 -0.3 6,202,899 - Pf6N2E2_5397 0.39 +1.4 6,202,899 - Pf6N2E2_5397 0.39 +0.9 6,202,899 - Pf6N2E2_5397 0.39 -1.4 6,202,899 - Pf6N2E2_5397 0.39 +0.7 6,202,900 + Pf6N2E2_5397 0.40 +0.2 6,202,900 + Pf6N2E2_5397 0.40 -0.8 6,202,900 + Pf6N2E2_5397 0.40 -1.4 6,202,900 + Pf6N2E2_5397 0.40 +0.2 6,202,900 + Pf6N2E2_5397 0.40 +1.0 6,202,900 + Pf6N2E2_5397 0.40 -0.3 6,202,900 + Pf6N2E2_5397 0.40 -0.8 6,202,900 + Pf6N2E2_5397 0.40 +0.4 6,202,900 + Pf6N2E2_5397 0.40 +0.2 6,202,900 + Pf6N2E2_5397 0.40 -0.4 6,202,901 - Pf6N2E2_5397 0.40 -0.3 6,202,901 - Pf6N2E2_5397 0.40 +0.5 6,202,901 - Pf6N2E2_5397 0.40 +1.2 6,202,901 - Pf6N2E2_5397 0.40 -0.3 6,202,901 - Pf6N2E2_5397 0.40 -0.2 6,202,901 - Pf6N2E2_5397 0.40 +0.7 6,202,901 - Pf6N2E2_5397 0.40 +0.2 6,202,901 - Pf6N2E2_5397 0.40 +0.9 6,202,901 - Pf6N2E2_5397 0.40 -0.4 6,203,178 - Pf6N2E2_5397 0.69 +1.3 6,203,178 - Pf6N2E2_5397 0.69 +0.5 6,203,188 + Pf6N2E2_5397 0.70 +0.2 6,203,188 + Pf6N2E2_5397 0.70 +0.4 6,203,189 - Pf6N2E2_5397 0.70 -0.8 6,203,237 - Pf6N2E2_5397 0.75 +1.4 6,203,237 - Pf6N2E2_5397 0.75 +1.0 6,203,237 - Pf6N2E2_5397 0.75 +0.6 6,203,237 - Pf6N2E2_5397 0.75 +0.2 6,203,237 - Pf6N2E2_5397 0.75 +0.1 6,203,237 - Pf6N2E2_5397 0.75 +0.2 6,203,237 - Pf6N2E2_5397 0.75 -2.0 6,203,342 - Pf6N2E2_5397 0.86 +1.2
Or see this region's nucleotide sequence