Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_496

Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_495 and Pf6N2E2_496 are separated by 183 nucleotidesPf6N2E2_496 and Pf6N2E2_497 are separated by 0 nucleotides Pf6N2E2_495 - YD repeat protein, at 561,448 to 562,563 _495 Pf6N2E2_496 - Dihydropyrimidine dehydrogenase (NADP(+)) (EC 1.3.1.2) (from data), at 562,747 to 564,021 _496 Pf6N2E2_497 - Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism, at 564,022 to 565,389 _497 Position (kb) 562 563 564 565Strain fitness (log2 ratio) -1 0 1 2at 561.834 kb on + strand, within Pf6N2E2_495at 562.001 kb on + strand, within Pf6N2E2_495at 562.085 kb on + strand, within Pf6N2E2_495at 562.184 kb on + strand, within Pf6N2E2_495at 562.184 kb on + strand, within Pf6N2E2_495at 562.607 kb on + strandat 562.625 kb on + strandat 562.628 kb on + strandat 562.640 kb on + strandat 562.640 kb on + strandat 562.640 kb on + strandat 562.640 kb on + strandat 562.641 kb on - strandat 562.764 kb on - strandat 562.867 kb on - strandat 562.892 kb on - strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.898 kb on + strand, within Pf6N2E2_496at 562.899 kb on - strand, within Pf6N2E2_496at 562.902 kb on + strand, within Pf6N2E2_496at 562.902 kb on + strand, within Pf6N2E2_496at 562.902 kb on + strand, within Pf6N2E2_496at 562.903 kb on - strandat 562.903 kb on - strand, within Pf6N2E2_496at 562.903 kb on - strand, within Pf6N2E2_496at 562.903 kb on - strand, within Pf6N2E2_496at 562.903 kb on - strand, within Pf6N2E2_496at 562.903 kb on - strand, within Pf6N2E2_496at 563.021 kb on + strand, within Pf6N2E2_496at 563.021 kb on + strand, within Pf6N2E2_496at 563.022 kb on - strand, within Pf6N2E2_496at 563.022 kb on - strand, within Pf6N2E2_496at 563.022 kb on - strand, within Pf6N2E2_496at 563.036 kb on + strand, within Pf6N2E2_496at 563.036 kb on + strand, within Pf6N2E2_496at 563.036 kb on + strand, within Pf6N2E2_496at 563.522 kb on + strand, within Pf6N2E2_496at 563.522 kb on + strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.555 kb on + strand, within Pf6N2E2_496at 563.555 kb on + strand, within Pf6N2E2_496at 563.556 kb on - strand, within Pf6N2E2_496at 563.556 kb on - strand, within Pf6N2E2_496at 563.843 kb on + strand, within Pf6N2E2_496at 563.936 kb on + strandat 563.937 kb on - strandat 563.937 kb on - strandat 563.937 kb on - strandat 563.937 kb on - strandat 563.937 kb on - strandat 564.024 kb on - strandat 564.141 kb on - strandat 564.141 kb on - strandat 564.617 kb on + strand, within Pf6N2E2_497at 564.617 kb on + strand, within Pf6N2E2_497at 564.815 kb on + strand, within Pf6N2E2_497at 564.815 kb on + strand, within Pf6N2E2_497at 564.833 kb on + strand, within Pf6N2E2_497at 564.834 kb on - strand, within Pf6N2E2_497at 564.834 kb on - strand, within Pf6N2E2_497at 564.834 kb on - strand, within Pf6N2E2_497

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Per-strain Table

Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
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561,834 + Pf6N2E2_495 0.35 -0.2
562,001 + Pf6N2E2_495 0.50 +0.9
562,085 + Pf6N2E2_495 0.57 +0.5
562,184 + Pf6N2E2_495 0.66 -1.3
562,184 + Pf6N2E2_495 0.66 -0.1
562,607 + +0.5
562,625 + -0.6
562,628 + +0.7
562,640 + -0.9
562,640 + +0.5
562,640 + -1.6
562,640 + -0.3
562,641 - -0.3
562,764 - -0.6
562,867 - -0.3
562,892 - Pf6N2E2_496 0.11 -0.1
562,898 + Pf6N2E2_496 0.12 +0.0
562,898 + Pf6N2E2_496 0.12 -0.6
562,898 + Pf6N2E2_496 0.12 -0.4
562,898 + Pf6N2E2_496 0.12 -0.3
562,898 + Pf6N2E2_496 0.12 +0.7
562,898 + Pf6N2E2_496 0.12 +0.2
562,899 - Pf6N2E2_496 0.12 +0.7
562,902 + Pf6N2E2_496 0.12 +0.4
562,902 + Pf6N2E2_496 0.12 -0.7
562,902 + Pf6N2E2_496 0.12 -0.3
562,903 - -0.3
562,903 - Pf6N2E2_496 0.12 +0.7
562,903 - Pf6N2E2_496 0.12 -0.3
562,903 - Pf6N2E2_496 0.12 -0.9
562,903 - Pf6N2E2_496 0.12 -0.4
562,903 - Pf6N2E2_496 0.12 -0.6
563,021 + Pf6N2E2_496 0.21 -0.5
563,021 + Pf6N2E2_496 0.21 -0.3
563,022 - Pf6N2E2_496 0.22 +1.3
563,022 - Pf6N2E2_496 0.22 +0.7
563,022 - Pf6N2E2_496 0.22 -0.3
563,036 + Pf6N2E2_496 0.23 +0.3
563,036 + Pf6N2E2_496 0.23 +0.7
563,036 + Pf6N2E2_496 0.23 +0.3
563,522 + Pf6N2E2_496 0.61 -0.3
563,522 + Pf6N2E2_496 0.61 -0.3
563,523 - Pf6N2E2_496 0.61 -0.3
563,523 - Pf6N2E2_496 0.61 -0.3
563,523 - Pf6N2E2_496 0.61 -0.5
563,523 - Pf6N2E2_496 0.61 -0.8
563,523 - Pf6N2E2_496 0.61 +0.3
563,523 - Pf6N2E2_496 0.61 -1.3
563,555 + Pf6N2E2_496 0.63 -0.6
563,555 + Pf6N2E2_496 0.63 +0.7
563,556 - Pf6N2E2_496 0.63 -1.1
563,556 - Pf6N2E2_496 0.63 -1.3
563,843 + Pf6N2E2_496 0.86 -1.3
563,936 + -0.1
563,937 - -1.3
563,937 - +2.0
563,937 - -0.5
563,937 - +0.0
563,937 - -0.9
564,024 - +0.0
564,141 - +1.8
564,141 - +0.7
564,617 + Pf6N2E2_497 0.43 -0.6
564,617 + Pf6N2E2_497 0.43 -0.3
564,815 + Pf6N2E2_497 0.58 +0.1
564,815 + Pf6N2E2_497 0.58 -0.8
564,833 + Pf6N2E2_497 0.59 -0.3
564,834 - Pf6N2E2_497 0.59 +0.7
564,834 - Pf6N2E2_497 0.59 -0.3
564,834 - Pf6N2E2_497 0.59 -1.0

Or see this region's nucleotide sequence