Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4437

Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4435 and Pf6N2E2_4436 are separated by 59 nucleotidesPf6N2E2_4436 and Pf6N2E2_4437 are separated by 191 nucleotidesPf6N2E2_4437 and Pf6N2E2_4438 overlap by 4 nucleotidesPf6N2E2_4438 and Pf6N2E2_4439 are separated by 50 nucleotides Pf6N2E2_4435 - Regulator of sigma D, at 5,167,567 to 5,168,025 _4435 Pf6N2E2_4436 - Macrophage infectivity potentiator, at 5,168,085 to 5,168,750 _4436 Pf6N2E2_4437 - Twin-arginine translocation protein TatB, at 5,168,942 to 5,170,117 _4437 Pf6N2E2_4438 - hypothetical protein, at 5,170,114 to 5,170,578 _4438 Pf6N2E2_4439 - Glutathione-regulated potassium-efflux system ATP-binding protein, at 5,170,629 to 5,172,539 _4439 Position (kb) 5168 5169 5170 5171Strain fitness (log2 ratio) -1 0 1 2at 5168.167 kb on + strand, within Pf6N2E2_4436at 5168.167 kb on + strand, within Pf6N2E2_4436at 5168.319 kb on - strand, within Pf6N2E2_4436at 5168.320 kb on + strand, within Pf6N2E2_4436at 5168.320 kb on + strand, within Pf6N2E2_4436at 5168.320 kb on + strand, within Pf6N2E2_4436at 5168.320 kb on + strand, within Pf6N2E2_4436at 5168.320 kb on + strand, within Pf6N2E2_4436at 5168.320 kb on + strand, within Pf6N2E2_4436at 5168.320 kb on + strand, within Pf6N2E2_4436at 5168.320 kb on + strand, within Pf6N2E2_4436at 5168.320 kb on + strand, within Pf6N2E2_4436at 5168.320 kb on + strand, within Pf6N2E2_4436at 5168.321 kb on - strand, within Pf6N2E2_4436at 5168.321 kb on - strand, within Pf6N2E2_4436at 5168.321 kb on - strand, within Pf6N2E2_4436at 5168.321 kb on - strand, within Pf6N2E2_4436at 5168.321 kb on - strand, within Pf6N2E2_4436at 5168.566 kb on + strand, within Pf6N2E2_4436at 5168.566 kb on + strand, within Pf6N2E2_4436at 5168.567 kb on - strand, within Pf6N2E2_4436at 5168.567 kb on - strand, within Pf6N2E2_4436at 5168.567 kb on - strand, within Pf6N2E2_4436at 5168.567 kb on - strand, within Pf6N2E2_4436at 5168.656 kb on + strand, within Pf6N2E2_4436at 5168.657 kb on - strand, within Pf6N2E2_4436at 5168.657 kb on - strand, within Pf6N2E2_4436at 5168.657 kb on - strand, within Pf6N2E2_4436at 5168.731 kb on - strandat 5168.780 kb on + strandat 5168.780 kb on + strandat 5168.781 kb on - strandat 5168.781 kb on - strandat 5168.781 kb on - strandat 5168.781 kb on - strandat 5168.865 kb on + strandat 5168.865 kb on + strandat 5168.865 kb on + strandat 5168.866 kb on - strandat 5168.904 kb on + strandat 5168.904 kb on + strandat 5168.904 kb on + strandat 5168.904 kb on + strandat 5168.904 kb on + strandat 5168.904 kb on + strandat 5168.904 kb on + strandat 5168.904 kb on + strandat 5168.904 kb on + strandat 5168.904 kb on + strandat 5168.904 kb on + strandat 5168.904 kb on + strandat 5168.904 kb on + strandat 5168.905 kb on - strandat 5168.905 kb on - strandat 5168.905 kb on - strandat 5168.905 kb on - strandat 5168.905 kb on - strandat 5168.907 kb on + strandat 5168.907 kb on + strandat 5168.907 kb on + strandat 5168.907 kb on + strandat 5168.907 kb on + strandat 5168.908 kb on - strandat 5168.908 kb on - strandat 5168.908 kb on - strandat 5168.908 kb on - strandat 5168.936 kb on + strandat 5168.937 kb on - strandat 5168.937 kb on - strandat 5168.937 kb on - strandat 5168.964 kb on + strandat 5168.965 kb on - strandat 5168.965 kb on - strandat 5169.242 kb on + strand, within Pf6N2E2_4437at 5169.242 kb on + strand, within Pf6N2E2_4437at 5169.242 kb on + strand, within Pf6N2E2_4437at 5169.242 kb on + strand, within Pf6N2E2_4437at 5169.242 kb on + strand, within Pf6N2E2_4437at 5169.243 kb on - strand, within Pf6N2E2_4437at 5169.243 kb on - strand, within Pf6N2E2_4437at 5169.243 kb on - strand, within Pf6N2E2_4437at 5169.243 kb on - strand, within Pf6N2E2_4437at 5169.243 kb on - strand, within Pf6N2E2_4437at 5169.598 kb on + strand, within Pf6N2E2_4437at 5169.598 kb on + strand, within Pf6N2E2_4437at 5169.611 kb on - strand, within Pf6N2E2_4437at 5169.647 kb on - strand, within Pf6N2E2_4437at 5169.685 kb on - strand, within Pf6N2E2_4437at 5170.115 kb on + strandat 5170.115 kb on + strandat 5170.116 kb on - strandat 5170.405 kb on + strand, within Pf6N2E2_4438at 5170.405 kb on + strand, within Pf6N2E2_4438at 5170.405 kb on + strand, within Pf6N2E2_4438at 5170.406 kb on - strand, within Pf6N2E2_4438at 5170.406 kb on - strand, within Pf6N2E2_4438at 5170.525 kb on + strand, within Pf6N2E2_4438at 5170.526 kb on - strand, within Pf6N2E2_4438at 5170.541 kb on + strandat 5170.541 kb on + strandat 5170.541 kb on + strandat 5170.541 kb on + strandat 5170.584 kb on + strandat 5170.612 kb on + strandat 5170.612 kb on + strandat 5170.628 kb on + strandat 5170.629 kb on - strandat 5170.629 kb on - strandat 5170.654 kb on + strandat 5170.654 kb on + strandat 5170.655 kb on - strandat 5170.678 kb on + strandat 5170.678 kb on + strandat 5170.679 kb on - strandat 5170.679 kb on - strandat 5170.875 kb on + strand, within Pf6N2E2_4439at 5170.875 kb on + strand, within Pf6N2E2_4439at 5170.875 kb on + strand, within Pf6N2E2_4439at 5170.875 kb on + strand, within Pf6N2E2_4439at 5170.876 kb on - strand, within Pf6N2E2_4439at 5170.876 kb on - strand, within Pf6N2E2_4439at 5170.995 kb on + strand, within Pf6N2E2_4439at 5170.996 kb on - strand, within Pf6N2E2_4439at 5171.042 kb on + strand, within Pf6N2E2_4439

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Per-strain Table

Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
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5,168,167 + Pf6N2E2_4436 0.12 +0.6
5,168,167 + Pf6N2E2_4436 0.12 +0.8
5,168,319 - Pf6N2E2_4436 0.35 +0.2
5,168,320 + Pf6N2E2_4436 0.35 -0.2
5,168,320 + Pf6N2E2_4436 0.35 +0.8
5,168,320 + Pf6N2E2_4436 0.35 +0.1
5,168,320 + Pf6N2E2_4436 0.35 +0.3
5,168,320 + Pf6N2E2_4436 0.35 -1.0
5,168,320 + Pf6N2E2_4436 0.35 +0.3
5,168,320 + Pf6N2E2_4436 0.35 +1.3
5,168,320 + Pf6N2E2_4436 0.35 -0.3
5,168,320 + Pf6N2E2_4436 0.35 -0.5
5,168,320 + Pf6N2E2_4436 0.35 -0.4
5,168,321 - Pf6N2E2_4436 0.35 -0.3
5,168,321 - Pf6N2E2_4436 0.35 +0.9
5,168,321 - Pf6N2E2_4436 0.35 -0.4
5,168,321 - Pf6N2E2_4436 0.35 +0.3
5,168,321 - Pf6N2E2_4436 0.35 +0.3
5,168,566 + Pf6N2E2_4436 0.72 +0.0
5,168,566 + Pf6N2E2_4436 0.72 -0.1
5,168,567 - Pf6N2E2_4436 0.72 +0.9
5,168,567 - Pf6N2E2_4436 0.72 -0.2
5,168,567 - Pf6N2E2_4436 0.72 -0.1
5,168,567 - Pf6N2E2_4436 0.72 +1.4
5,168,656 + Pf6N2E2_4436 0.86 +0.3
5,168,657 - Pf6N2E2_4436 0.86 +0.7
5,168,657 - Pf6N2E2_4436 0.86 -0.7
5,168,657 - Pf6N2E2_4436 0.86 +0.3
5,168,731 - +1.3
5,168,780 + +0.3
5,168,780 + -0.7
5,168,781 - -0.7
5,168,781 - -0.0
5,168,781 - -0.3
5,168,781 - +0.3
5,168,865 + +0.3
5,168,865 + +0.3
5,168,865 + +0.3
5,168,866 - -0.0
5,168,904 + +1.2
5,168,904 + -0.4
5,168,904 + +0.9
5,168,904 + +0.3
5,168,904 + +1.5
5,168,904 + +0.5
5,168,904 + +0.7
5,168,904 + +0.1
5,168,904 + +0.3
5,168,904 + +0.3
5,168,904 + +0.6
5,168,904 + -0.3
5,168,904 + +0.9
5,168,905 - -0.2
5,168,905 - +0.3
5,168,905 - +0.5
5,168,905 - +1.7
5,168,905 - +0.8
5,168,907 + -0.4
5,168,907 + +0.3
5,168,907 + +0.3
5,168,907 + -0.3
5,168,907 + +0.8
5,168,908 - +0.2
5,168,908 - +0.1
5,168,908 - -0.7
5,168,908 - +0.6
5,168,936 + +1.3
5,168,937 - +1.9
5,168,937 - -0.2
5,168,937 - +0.3
5,168,964 + +0.7
5,168,965 - +0.3
5,168,965 - +1.3
5,169,242 + Pf6N2E2_4437 0.26 -0.7
5,169,242 + Pf6N2E2_4437 0.26 +0.3
5,169,242 + Pf6N2E2_4437 0.26 +0.3
5,169,242 + Pf6N2E2_4437 0.26 +0.3
5,169,242 + Pf6N2E2_4437 0.26 -0.3
5,169,243 - Pf6N2E2_4437 0.26 +0.3
5,169,243 - Pf6N2E2_4437 0.26 -0.1
5,169,243 - Pf6N2E2_4437 0.26 +0.1
5,169,243 - Pf6N2E2_4437 0.26 -0.1
5,169,243 - Pf6N2E2_4437 0.26 -0.7
5,169,598 + Pf6N2E2_4437 0.56 -0.9
5,169,598 + Pf6N2E2_4437 0.56 +0.5
5,169,611 - Pf6N2E2_4437 0.57 +2.7
5,169,647 - Pf6N2E2_4437 0.60 +0.8
5,169,685 - Pf6N2E2_4437 0.63 -0.0
5,170,115 + -0.0
5,170,115 + +0.3
5,170,116 - -1.2
5,170,405 + Pf6N2E2_4438 0.63 +0.3
5,170,405 + Pf6N2E2_4438 0.63 -0.7
5,170,405 + Pf6N2E2_4438 0.63 +0.9
5,170,406 - Pf6N2E2_4438 0.63 -0.1
5,170,406 - Pf6N2E2_4438 0.63 +0.8
5,170,525 + Pf6N2E2_4438 0.88 -0.5
5,170,526 - Pf6N2E2_4438 0.89 +0.3
5,170,541 + -1.0
5,170,541 + +0.3
5,170,541 + -1.1
5,170,541 + -1.5
5,170,584 + -0.7
5,170,612 + +1.9
5,170,612 + +2.3
5,170,628 + -1.4
5,170,629 - +1.2
5,170,629 - -0.5
5,170,654 + -0.1
5,170,654 + -1.1
5,170,655 - -0.4
5,170,678 + +0.2
5,170,678 + +0.9
5,170,679 - -0.2
5,170,679 - +1.7
5,170,875 + Pf6N2E2_4439 0.13 -0.4
5,170,875 + Pf6N2E2_4439 0.13 -1.0
5,170,875 + Pf6N2E2_4439 0.13 -0.8
5,170,875 + Pf6N2E2_4439 0.13 -1.7
5,170,876 - Pf6N2E2_4439 0.13 -0.1
5,170,876 - Pf6N2E2_4439 0.13 +0.3
5,170,995 + Pf6N2E2_4439 0.19 +0.3
5,170,996 - Pf6N2E2_4439 0.19 +0.9
5,171,042 + Pf6N2E2_4439 0.22 +0.5

Or see this region's nucleotide sequence