Experiment: 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1226 and Pf6N2E2_1227 are separated by 220 nucleotides Pf6N2E2_1227 and Pf6N2E2_1228 are separated by 128 nucleotides Pf6N2E2_1228 and Pf6N2E2_1229 are separated by 169 nucleotides
Pf6N2E2_1226 - DNA damage response nuclease (from data) , at 1,434,468 to 1,436,138
_1226
Pf6N2E2_1227 - FIG00955097: hypothetical protein, at 1,436,359 to 1,436,583
_1227
Pf6N2E2_1228 - Lipase (EC 3.1.1.3), at 1,436,712 to 1,438,565
_1228
Pf6N2E2_1229 - Serine protease homologue, at 1,438,735 to 1,441,827
_1229
Position (kb)
1436
1437
1438
1439 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1436.104 kb on + strand at 1436.104 kb on + strand at 1436.107 kb on + strand at 1436.107 kb on + strand at 1436.108 kb on - strand at 1436.108 kb on - strand at 1436.191 kb on + strand at 1436.229 kb on + strand at 1436.231 kb on + strand at 1436.231 kb on + strand at 1436.231 kb on + strand at 1436.232 kb on - strand at 1436.232 kb on - strand at 1436.232 kb on - strand at 1436.235 kb on + strand at 1436.235 kb on + strand at 1436.236 kb on - strand at 1436.236 kb on - strand at 1436.236 kb on - strand at 1436.352 kb on + strand at 1436.461 kb on + strand, within Pf6N2E2_1227 at 1436.652 kb on + strand at 1436.652 kb on + strand at 1436.653 kb on - strand at 1436.658 kb on + strand at 1436.659 kb on - strand at 1436.665 kb on + strand at 1436.665 kb on + strand at 1436.666 kb on - strand at 1436.821 kb on + strand at 1436.821 kb on + strand at 1436.822 kb on - strand at 1436.830 kb on + strand at 1437.106 kb on + strand, within Pf6N2E2_1228 at 1437.106 kb on + strand, within Pf6N2E2_1228 at 1437.106 kb on + strand, within Pf6N2E2_1228 at 1437.107 kb on - strand, within Pf6N2E2_1228 at 1437.107 kb on - strand, within Pf6N2E2_1228 at 1437.107 kb on - strand, within Pf6N2E2_1228 at 1437.259 kb on + strand, within Pf6N2E2_1228 at 1437.259 kb on + strand, within Pf6N2E2_1228 at 1437.260 kb on - strand, within Pf6N2E2_1228 at 1437.260 kb on - strand, within Pf6N2E2_1228 at 1437.323 kb on + strand, within Pf6N2E2_1228 at 1437.323 kb on + strand, within Pf6N2E2_1228 at 1437.323 kb on + strand, within Pf6N2E2_1228 at 1437.324 kb on - strand, within Pf6N2E2_1228 at 1437.324 kb on - strand, within Pf6N2E2_1228 at 1437.324 kb on - strand, within Pf6N2E2_1228 at 1437.324 kb on - strand, within Pf6N2E2_1228 at 1437.324 kb on - strand, within Pf6N2E2_1228 at 1437.326 kb on + strand, within Pf6N2E2_1228 at 1437.326 kb on + strand, within Pf6N2E2_1228 at 1437.327 kb on - strand, within Pf6N2E2_1228 at 1437.353 kb on + strand, within Pf6N2E2_1228 at 1437.354 kb on - strand, within Pf6N2E2_1228 at 1437.354 kb on - strand, within Pf6N2E2_1228 at 1437.355 kb on + strand, within Pf6N2E2_1228 at 1437.355 kb on + strand, within Pf6N2E2_1228 at 1437.509 kb on + strand, within Pf6N2E2_1228 at 1437.509 kb on + strand, within Pf6N2E2_1228 at 1437.510 kb on - strand, within Pf6N2E2_1228 at 1437.575 kb on - strand, within Pf6N2E2_1228 at 1437.691 kb on + strand, within Pf6N2E2_1228 at 1437.691 kb on + strand, within Pf6N2E2_1228 at 1437.868 kb on + strand, within Pf6N2E2_1228 at 1437.883 kb on + strand, within Pf6N2E2_1228 at 1438.055 kb on - strand, within Pf6N2E2_1228 at 1438.055 kb on - strand, within Pf6N2E2_1228 at 1438.186 kb on + strand, within Pf6N2E2_1228 at 1438.204 kb on - strand, within Pf6N2E2_1228 at 1438.220 kb on - strand, within Pf6N2E2_1228 at 1438.220 kb on - strand, within Pf6N2E2_1228 at 1438.480 kb on + strand at 1438.481 kb on - strand at 1438.481 kb on - strand at 1438.481 kb on - strand at 1438.486 kb on + strand at 1438.486 kb on + strand at 1438.486 kb on + strand at 1438.487 kb on - strand at 1438.549 kb on + strand at 1438.549 kb on + strand at 1438.549 kb on + strand at 1438.550 kb on - strand at 1438.550 kb on - strand at 1438.555 kb on + strand at 1438.555 kb on + strand at 1438.555 kb on + strand at 1438.555 kb on + strand at 1438.559 kb on + strand at 1438.559 kb on + strand at 1438.559 kb on + strand at 1438.560 kb on - strand at 1438.596 kb on + strand at 1438.597 kb on - strand at 1438.597 kb on - strand at 1438.609 kb on + strand at 1438.610 kb on - strand at 1438.652 kb on + strand at 1438.652 kb on + strand at 1438.652 kb on + strand at 1438.802 kb on + strand at 1438.803 kb on - strand at 1438.803 kb on - strand at 1438.987 kb on + strand at 1438.987 kb on + strand at 1438.987 kb on + strand at 1438.988 kb on - strand at 1438.988 kb on - strand at 1438.988 kb on - strand at 1438.988 kb on - strand at 1438.988 kb on - strand at 1438.988 kb on - strand at 1439.016 kb on + strand at 1439.017 kb on - strand at 1439.129 kb on + strand, within Pf6N2E2_1229 at 1439.129 kb on + strand, within Pf6N2E2_1229 at 1439.129 kb on + strand, within Pf6N2E2_1229 at 1439.130 kb on - strand, within Pf6N2E2_1229 at 1439.130 kb on - strand, within Pf6N2E2_1229 at 1439.130 kb on - strand, within Pf6N2E2_1229 at 1439.130 kb on - strand, within Pf6N2E2_1229 at 1439.130 kb on - strand, within Pf6N2E2_1229 at 1439.354 kb on + strand, within Pf6N2E2_1229 at 1439.355 kb on - strand, within Pf6N2E2_1229 at 1439.414 kb on + strand, within Pf6N2E2_1229 at 1439.497 kb on - strand, within Pf6N2E2_1229
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 1,436,104 + -1.2 1,436,104 + -1.7 1,436,107 + -0.2 1,436,107 + -2.7 1,436,108 - +1.3 1,436,108 - -0.4 1,436,191 + +0.3 1,436,229 + -0.4 1,436,231 + -0.8 1,436,231 + -0.2 1,436,231 + +0.2 1,436,232 - -1.4 1,436,232 - +1.7 1,436,232 - +0.3 1,436,235 + +0.3 1,436,235 + +0.3 1,436,236 - -0.7 1,436,236 - -0.7 1,436,236 - +1.9 1,436,352 + -0.7 1,436,461 + Pf6N2E2_1227 0.45 +0.9 1,436,652 + -0.7 1,436,652 + +0.8 1,436,653 - -0.7 1,436,658 + +0.3 1,436,659 - -0.7 1,436,665 + +1.1 1,436,665 + +0.4 1,436,666 - +1.5 1,436,821 + +2.1 1,436,821 + +0.3 1,436,822 - -0.7 1,436,830 + -0.7 1,437,106 + Pf6N2E2_1228 0.21 -2.2 1,437,106 + Pf6N2E2_1228 0.21 +1.4 1,437,106 + Pf6N2E2_1228 0.21 +0.1 1,437,107 - Pf6N2E2_1228 0.21 -0.5 1,437,107 - Pf6N2E2_1228 0.21 -0.2 1,437,107 - Pf6N2E2_1228 0.21 +0.3 1,437,259 + Pf6N2E2_1228 0.30 -0.4 1,437,259 + Pf6N2E2_1228 0.30 -0.1 1,437,260 - Pf6N2E2_1228 0.30 -1.4 1,437,260 - Pf6N2E2_1228 0.30 -0.1 1,437,323 + Pf6N2E2_1228 0.33 +0.3 1,437,323 + Pf6N2E2_1228 0.33 -1.7 1,437,323 + Pf6N2E2_1228 0.33 -0.5 1,437,324 - Pf6N2E2_1228 0.33 +0.3 1,437,324 - Pf6N2E2_1228 0.33 +1.5 1,437,324 - Pf6N2E2_1228 0.33 +0.5 1,437,324 - Pf6N2E2_1228 0.33 -0.7 1,437,324 - Pf6N2E2_1228 0.33 -1.1 1,437,326 + Pf6N2E2_1228 0.33 -1.1 1,437,326 + Pf6N2E2_1228 0.33 +1.7 1,437,327 - Pf6N2E2_1228 0.33 -0.4 1,437,353 + Pf6N2E2_1228 0.35 +1.8 1,437,354 - Pf6N2E2_1228 0.35 +1.1 1,437,354 - Pf6N2E2_1228 0.35 -0.7 1,437,355 + Pf6N2E2_1228 0.35 -0.7 1,437,355 + Pf6N2E2_1228 0.35 -1.2 1,437,509 + Pf6N2E2_1228 0.43 -0.7 1,437,509 + Pf6N2E2_1228 0.43 -0.4 1,437,510 - Pf6N2E2_1228 0.43 +1.0 1,437,575 - Pf6N2E2_1228 0.47 -0.7 1,437,691 + Pf6N2E2_1228 0.53 +0.3 1,437,691 + Pf6N2E2_1228 0.53 -0.3 1,437,868 + Pf6N2E2_1228 0.62 -2.8 1,437,883 + Pf6N2E2_1228 0.63 +0.7 1,438,055 - Pf6N2E2_1228 0.72 -1.2 1,438,055 - Pf6N2E2_1228 0.72 -0.7 1,438,186 + Pf6N2E2_1228 0.80 -0.1 1,438,204 - Pf6N2E2_1228 0.80 +0.4 1,438,220 - Pf6N2E2_1228 0.81 -0.7 1,438,220 - Pf6N2E2_1228 0.81 -1.2 1,438,480 + +0.3 1,438,481 - +0.3 1,438,481 - +0.2 1,438,481 - -0.7 1,438,486 + -0.9 1,438,486 + -0.4 1,438,486 + -0.1 1,438,487 - -0.7 1,438,549 + -0.9 1,438,549 + +0.1 1,438,549 + +1.3 1,438,550 - -0.3 1,438,550 - +2.7 1,438,555 + +0.2 1,438,555 + -0.2 1,438,555 + -0.0 1,438,555 + -0.7 1,438,559 + +0.3 1,438,559 + +0.1 1,438,559 + -1.7 1,438,560 - -1.0 1,438,596 + -2.2 1,438,597 - -0.7 1,438,597 - -1.2 1,438,609 + -1.0 1,438,610 - -1.0 1,438,652 + -1.7 1,438,652 + +0.6 1,438,652 + -0.0 1,438,802 + -0.7 1,438,803 - -0.4 1,438,803 - +0.2 1,438,987 + +0.3 1,438,987 + +0.3 1,438,987 + -0.0 1,438,988 - -1.1 1,438,988 - -0.7 1,438,988 - +1.7 1,438,988 - +0.3 1,438,988 - +0.7 1,438,988 - -0.9 1,439,016 + -0.7 1,439,017 - +0.1 1,439,129 + Pf6N2E2_1229 0.13 +0.3 1,439,129 + Pf6N2E2_1229 0.13 -0.9 1,439,129 + Pf6N2E2_1229 0.13 +0.7 1,439,130 - Pf6N2E2_1229 0.13 -0.8 1,439,130 - Pf6N2E2_1229 0.13 -0.7 1,439,130 - Pf6N2E2_1229 0.13 +0.7 1,439,130 - Pf6N2E2_1229 0.13 -0.7 1,439,130 - Pf6N2E2_1229 0.13 -0.2 1,439,354 + Pf6N2E2_1229 0.20 +0.1 1,439,355 - Pf6N2E2_1229 0.20 -0.7 1,439,414 + Pf6N2E2_1229 0.22 +0.2 1,439,497 - Pf6N2E2_1229 0.25 -0.2
Or see this region's nucleotide sequence