Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27160 and BWI76_RS27165 are separated by 127 nucleotides BWI76_RS27165 and BWI76_RS27170 overlap by 4 nucleotides BWI76_RS27170 and BWI76_RS27175 overlap by 12 nucleotides
BWI76_RS27160: BWI76_RS27160 - PTS mannitol transporter subunit IICBA, at 5,638,012 to 5,639,922
_RS27160
BWI76_RS27165: BWI76_RS27165 - mannitol-1-phosphate 5-dehydrogenase, at 5,640,050 to 5,641,198
_RS27165
BWI76_RS27170: BWI76_RS27170 - MltR family transcriptional regulator, at 5,641,195 to 5,641,791
_RS27170
BWI76_RS27175: BWI76_RS27175 - hypothetical protein, at 5,641,780 to 5,642,004
_RS27175
Position (kb) 5640
5641
5642 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5639.292 kb on + strand, within BWI76_RS27160 at 5639.421 kb on + strand, within BWI76_RS27160 at 5639.483 kb on + strand, within BWI76_RS27160 at 5639.491 kb on - strand, within BWI76_RS27160 at 5639.585 kb on - strand, within BWI76_RS27160 at 5639.617 kb on + strand at 5639.625 kb on - strand, within BWI76_RS27160 at 5639.661 kb on - strand, within BWI76_RS27160 at 5639.666 kb on - strand, within BWI76_RS27160 at 5639.899 kb on + strand at 5639.899 kb on + strand at 5639.958 kb on + strand at 5639.978 kb on - strand at 5639.983 kb on + strand at 5639.984 kb on + strand at 5639.990 kb on - strand at 5639.994 kb on + strand at 5640.014 kb on - strand at 5640.225 kb on - strand, within BWI76_RS27165 at 5640.335 kb on + strand, within BWI76_RS27165 at 5640.444 kb on - strand, within BWI76_RS27165 at 5640.523 kb on - strand, within BWI76_RS27165 at 5640.613 kb on + strand, within BWI76_RS27165 at 5640.621 kb on - strand, within BWI76_RS27165 at 5640.621 kb on - strand, within BWI76_RS27165 at 5640.621 kb on - strand, within BWI76_RS27165 at 5640.686 kb on + strand, within BWI76_RS27165 at 5640.990 kb on - strand, within BWI76_RS27165 at 5640.996 kb on + strand, within BWI76_RS27165 at 5641.215 kb on + strand at 5641.434 kb on + strand, within BWI76_RS27170 at 5641.487 kb on + strand, within BWI76_RS27170 at 5641.495 kb on - strand, within BWI76_RS27170 at 5641.538 kb on - strand, within BWI76_RS27170 at 5641.549 kb on + strand, within BWI76_RS27170 at 5641.567 kb on + strand, within BWI76_RS27170 at 5641.570 kb on + strand, within BWI76_RS27170 at 5641.664 kb on + strand, within BWI76_RS27170 at 5641.705 kb on + strand, within BWI76_RS27170 at 5641.736 kb on - strand at 5641.736 kb on - strand at 5641.798 kb on - strand at 5641.839 kb on + strand, within BWI76_RS27175 at 5641.840 kb on - strand, within BWI76_RS27175 at 5641.889 kb on + strand, within BWI76_RS27175 at 5641.941 kb on - strand, within BWI76_RS27175 at 5642.008 kb on + strand at 5642.008 kb on + strand at 5642.011 kb on + strand at 5642.011 kb on + strand at 5642.013 kb on + strand at 5642.016 kb on - strand at 5642.016 kb on - strand at 5642.018 kb on + strand at 5642.019 kb on - strand at 5642.019 kb on - strand at 5642.019 kb on - strand at 5642.023 kb on + strand at 5642.031 kb on - strand at 5642.039 kb on + strand at 5642.085 kb on + strand at 5642.159 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 5,639,292 + BWI76_RS27160 0.67 -1.7 5,639,421 + BWI76_RS27160 0.74 -2.2 5,639,483 + BWI76_RS27160 0.77 -2.3 5,639,491 - BWI76_RS27160 0.77 +1.5 5,639,585 - BWI76_RS27160 0.82 -0.2 5,639,617 + -0.2 5,639,625 - BWI76_RS27160 0.84 -0.6 5,639,661 - BWI76_RS27160 0.86 -0.7 5,639,666 - BWI76_RS27160 0.87 -0.3 5,639,899 + -0.4 5,639,899 + -0.6 5,639,958 + +1.4 5,639,978 - -0.5 5,639,983 + +0.5 5,639,984 + +0.2 5,639,990 - -1.6 5,639,994 + +0.0 5,640,014 - +0.4 5,640,225 - BWI76_RS27165 0.15 +0.4 5,640,335 + BWI76_RS27165 0.25 -0.2 5,640,444 - BWI76_RS27165 0.34 -3.2 5,640,523 - BWI76_RS27165 0.41 -1.5 5,640,613 + BWI76_RS27165 0.49 +1.4 5,640,621 - BWI76_RS27165 0.50 -2.0 5,640,621 - BWI76_RS27165 0.50 -2.1 5,640,621 - BWI76_RS27165 0.50 -0.6 5,640,686 + BWI76_RS27165 0.55 -1.2 5,640,990 - BWI76_RS27165 0.82 +0.4 5,640,996 + BWI76_RS27165 0.82 -0.6 5,641,215 + -1.8 5,641,434 + BWI76_RS27170 0.40 -0.8 5,641,487 + BWI76_RS27170 0.49 +2.2 5,641,495 - BWI76_RS27170 0.50 -2.6 5,641,538 - BWI76_RS27170 0.57 -0.3 5,641,549 + BWI76_RS27170 0.59 +0.4 5,641,567 + BWI76_RS27170 0.62 +0.3 5,641,570 + BWI76_RS27170 0.63 -0.3 5,641,664 + BWI76_RS27170 0.79 +0.9 5,641,705 + BWI76_RS27170 0.85 -0.7 5,641,736 - +0.9 5,641,736 - -0.5 5,641,798 - +1.5 5,641,839 + BWI76_RS27175 0.26 -2.6 5,641,840 - BWI76_RS27175 0.27 +1.3 5,641,889 + BWI76_RS27175 0.48 -0.2 5,641,941 - BWI76_RS27175 0.72 -0.3 5,642,008 + +0.1 5,642,008 + -0.7 5,642,011 + +1.3 5,642,011 + +0.1 5,642,013 + +0.3 5,642,016 - +0.3 5,642,016 - -0.2 5,642,018 + -0.2 5,642,019 - +1.2 5,642,019 - +0.7 5,642,019 - -1.0 5,642,023 + -1.4 5,642,031 - -1.3 5,642,039 + +0.6 5,642,085 + +0.2 5,642,159 - +2.0
Or see this region's nucleotide sequence