Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26740 and BWI76_RS26745 overlap by 32 nucleotides BWI76_RS26745 and BWI76_RS26750 overlap by 8 nucleotides BWI76_RS26750 and BWI76_RS26755 are separated by 183 nucleotides
BWI76_RS26740: BWI76_RS26740 - AsmA family protein, at 5,537,398 to 5,539,449
_RS26740
BWI76_RS26745: BWI76_RS26745 - antibiotic biosynthesis monooxygenase, at 5,539,418 to 5,539,652
_RS26745
BWI76_RS26750: BWI76_RS26750 - PfkB domain-containing protein, at 5,539,645 to 5,540,580
_RS26750
BWI76_RS26755: BWI76_RS26755 - peptidase M16, at 5,540,764 to 5,542,269
_RS26755
Position (kb) 5539
5540
5541 Strain fitness (log2 ratio) -2
-1
0
1 at 5538.895 kb on + strand, within BWI76_RS26740 at 5538.903 kb on - strand, within BWI76_RS26740 at 5539.057 kb on + strand, within BWI76_RS26740 at 5539.369 kb on + strand at 5539.450 kb on - strand, within BWI76_RS26745 at 5539.452 kb on - strand, within BWI76_RS26745 at 5539.452 kb on - strand, within BWI76_RS26745 at 5539.479 kb on + strand, within BWI76_RS26745 at 5539.602 kb on + strand, within BWI76_RS26745 at 5539.610 kb on - strand, within BWI76_RS26745 at 5539.610 kb on - strand, within BWI76_RS26745 at 5539.614 kb on - strand, within BWI76_RS26745 at 5539.615 kb on - strand, within BWI76_RS26745 at 5539.732 kb on + strand at 5539.900 kb on + strand, within BWI76_RS26750 at 5539.900 kb on + strand, within BWI76_RS26750 at 5540.003 kb on - strand, within BWI76_RS26750 at 5540.009 kb on + strand, within BWI76_RS26750 at 5540.024 kb on - strand, within BWI76_RS26750 at 5540.295 kb on - strand, within BWI76_RS26750 at 5540.295 kb on - strand, within BWI76_RS26750 at 5540.635 kb on + strand at 5540.697 kb on + strand at 5540.941 kb on - strand, within BWI76_RS26755 at 5541.095 kb on - strand, within BWI76_RS26755 at 5541.284 kb on - strand, within BWI76_RS26755 at 5541.292 kb on + strand, within BWI76_RS26755 at 5541.426 kb on + strand, within BWI76_RS26755 at 5541.434 kb on - strand, within BWI76_RS26755 at 5541.484 kb on - strand, within BWI76_RS26755
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 5,538,895 + BWI76_RS26740 0.73 -0.3 5,538,903 - BWI76_RS26740 0.73 -1.1 5,539,057 + BWI76_RS26740 0.81 -2.2 5,539,369 + +1.4 5,539,450 - BWI76_RS26745 0.14 +0.9 5,539,452 - BWI76_RS26745 0.14 +0.1 5,539,452 - BWI76_RS26745 0.14 -2.4 5,539,479 + BWI76_RS26745 0.26 +0.5 5,539,602 + BWI76_RS26745 0.78 +1.2 5,539,610 - BWI76_RS26745 0.82 +0.4 5,539,610 - BWI76_RS26745 0.82 +0.4 5,539,614 - BWI76_RS26745 0.83 +0.4 5,539,615 - BWI76_RS26745 0.84 -0.4 5,539,732 + -0.5 5,539,900 + BWI76_RS26750 0.27 -1.0 5,539,900 + BWI76_RS26750 0.27 -0.3 5,540,003 - BWI76_RS26750 0.38 -0.8 5,540,009 + BWI76_RS26750 0.39 -1.0 5,540,024 - BWI76_RS26750 0.40 -0.2 5,540,295 - BWI76_RS26750 0.69 -1.6 5,540,295 - BWI76_RS26750 0.69 +0.5 5,540,635 + +0.1 5,540,697 + +1.4 5,540,941 - BWI76_RS26755 0.12 -0.4 5,541,095 - BWI76_RS26755 0.22 -1.2 5,541,284 - BWI76_RS26755 0.35 -0.4 5,541,292 + BWI76_RS26755 0.35 -0.5 5,541,426 + BWI76_RS26755 0.44 -0.1 5,541,434 - BWI76_RS26755 0.44 -0.9 5,541,484 - BWI76_RS26755 0.48 -1.0
Or see this region's nucleotide sequence