Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26475 and BWI76_RS26480 overlap by 4 nucleotides BWI76_RS26480 and BWI76_RS26485 are separated by 1 nucleotides
BWI76_RS26475: BWI76_RS26475 - bifunctional UDP-glucuronic acid oxidase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase, at 5,478,628 to 5,480,613
_RS26475
BWI76_RS26480: BWI76_RS26480 - undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase, at 5,480,610 to 5,481,593
_RS26480
BWI76_RS26485: BWI76_RS26485 - UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase, at 5,481,595 to 5,482,734
_RS26485
Position (kb) 5480
5481
5482 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5479.699 kb on + strand, within BWI76_RS26475 at 5479.707 kb on - strand, within BWI76_RS26475 at 5480.260 kb on + strand, within BWI76_RS26475 at 5480.307 kb on + strand at 5480.455 kb on + strand at 5480.515 kb on - strand at 5480.571 kb on - strand at 5480.726 kb on + strand, within BWI76_RS26480 at 5481.049 kb on + strand, within BWI76_RS26480 at 5481.054 kb on - strand, within BWI76_RS26480 at 5481.203 kb on - strand, within BWI76_RS26480 at 5481.598 kb on - strand at 5481.626 kb on - strand at 5481.650 kb on + strand at 5481.691 kb on + strand at 5481.766 kb on + strand, within BWI76_RS26485 at 5481.774 kb on - strand, within BWI76_RS26485 at 5481.791 kb on + strand, within BWI76_RS26485 at 5481.818 kb on - strand, within BWI76_RS26485 at 5481.871 kb on + strand, within BWI76_RS26485 at 5482.329 kb on + strand, within BWI76_RS26485 at 5482.348 kb on + strand, within BWI76_RS26485 at 5482.489 kb on + strand, within BWI76_RS26485 at 5482.529 kb on - strand, within BWI76_RS26485 at 5482.581 kb on - strand, within BWI76_RS26485
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 5,479,699 + BWI76_RS26475 0.54 +0.2 5,479,707 - BWI76_RS26475 0.54 -0.8 5,480,260 + BWI76_RS26475 0.82 -3.2 5,480,307 + +0.4 5,480,455 + +0.3 5,480,515 - +0.3 5,480,571 - +1.8 5,480,726 + BWI76_RS26480 0.12 +1.8 5,481,049 + BWI76_RS26480 0.45 +0.2 5,481,054 - BWI76_RS26480 0.45 +0.8 5,481,203 - BWI76_RS26480 0.60 +0.4 5,481,598 - +0.5 5,481,626 - +0.4 5,481,650 + -2.6 5,481,691 + -0.0 5,481,766 + BWI76_RS26485 0.15 +0.5 5,481,774 - BWI76_RS26485 0.16 -0.2 5,481,791 + BWI76_RS26485 0.17 +1.6 5,481,818 - BWI76_RS26485 0.20 +0.4 5,481,871 + BWI76_RS26485 0.24 +0.2 5,482,329 + BWI76_RS26485 0.64 +0.4 5,482,348 + BWI76_RS26485 0.66 -1.6 5,482,489 + BWI76_RS26485 0.78 +0.4 5,482,529 - BWI76_RS26485 0.82 +0.8 5,482,581 - BWI76_RS26485 0.86 -0.8
Or see this region's nucleotide sequence