Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26365 and BWI76_RS26370 are separated by 241 nucleotides BWI76_RS26370 and BWI76_RS26375 are separated by 150 nucleotides
BWI76_RS26365: BWI76_RS26365 - branched chain amino acid ABC transporter substrate-binding protein, at 5,456,522 to 5,457,640
_RS26365
BWI76_RS26370: BWI76_RS26370 - aromatic amino acid transporter, at 5,457,882 to 5,459,126
_RS26370
BWI76_RS26375: BWI76_RS26375 - aspartate aminotransferase family protein, at 5,459,277 to 5,460,542
_RS26375
Position (kb) 5457
5458
5459
5460 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5457.085 kb on - strand, within BWI76_RS26365 at 5457.092 kb on + strand, within BWI76_RS26365 at 5457.105 kb on + strand, within BWI76_RS26365 at 5457.262 kb on + strand, within BWI76_RS26365 at 5457.301 kb on - strand, within BWI76_RS26365 at 5457.599 kb on + strand at 5457.609 kb on - strand at 5457.808 kb on + strand at 5457.818 kb on - strand at 5457.818 kb on - strand at 5457.866 kb on + strand at 5457.911 kb on - strand at 5457.929 kb on - strand at 5458.108 kb on + strand, within BWI76_RS26370 at 5458.110 kb on + strand, within BWI76_RS26370 at 5458.110 kb on + strand, within BWI76_RS26370 at 5458.165 kb on + strand, within BWI76_RS26370 at 5458.174 kb on - strand, within BWI76_RS26370 at 5458.192 kb on + strand, within BWI76_RS26370 at 5458.210 kb on - strand, within BWI76_RS26370 at 5458.252 kb on - strand at 5458.369 kb on - strand, within BWI76_RS26370 at 5458.415 kb on - strand, within BWI76_RS26370 at 5458.416 kb on - strand, within BWI76_RS26370 at 5458.602 kb on - strand, within BWI76_RS26370 at 5458.614 kb on + strand, within BWI76_RS26370 at 5458.614 kb on + strand, within BWI76_RS26370 at 5458.622 kb on - strand, within BWI76_RS26370 at 5458.680 kb on - strand, within BWI76_RS26370 at 5458.903 kb on + strand, within BWI76_RS26370 at 5458.911 kb on - strand, within BWI76_RS26370 at 5459.069 kb on + strand at 5459.077 kb on - strand at 5459.314 kb on + strand at 5459.385 kb on - strand at 5459.523 kb on + strand, within BWI76_RS26375 at 5459.523 kb on + strand, within BWI76_RS26375 at 5459.531 kb on - strand, within BWI76_RS26375 at 5459.531 kb on - strand, within BWI76_RS26375 at 5459.541 kb on - strand, within BWI76_RS26375 at 5459.650 kb on + strand, within BWI76_RS26375 at 5459.669 kb on - strand, within BWI76_RS26375 at 5459.786 kb on - strand, within BWI76_RS26375 at 5459.788 kb on + strand, within BWI76_RS26375 at 5459.835 kb on + strand, within BWI76_RS26375 at 5460.070 kb on + strand at 5460.104 kb on + strand, within BWI76_RS26375 at 5460.111 kb on + strand, within BWI76_RS26375
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 5,457,085 - BWI76_RS26365 0.50 +0.6 5,457,092 + BWI76_RS26365 0.51 +0.1 5,457,105 + BWI76_RS26365 0.52 +0.2 5,457,262 + BWI76_RS26365 0.66 -1.4 5,457,301 - BWI76_RS26365 0.70 +0.6 5,457,599 + +0.9 5,457,609 - +0.1 5,457,808 + -0.8 5,457,818 - -3.0 5,457,818 - +0.4 5,457,866 + -1.4 5,457,911 - +1.1 5,457,929 - +1.4 5,458,108 + BWI76_RS26370 0.18 +1.8 5,458,110 + BWI76_RS26370 0.18 +2.1 5,458,110 + BWI76_RS26370 0.18 +0.6 5,458,165 + BWI76_RS26370 0.23 +1.1 5,458,174 - BWI76_RS26370 0.23 -0.5 5,458,192 + BWI76_RS26370 0.25 +0.7 5,458,210 - BWI76_RS26370 0.26 -1.1 5,458,252 - +1.4 5,458,369 - BWI76_RS26370 0.39 +1.2 5,458,415 - BWI76_RS26370 0.43 +1.1 5,458,416 - BWI76_RS26370 0.43 +1.0 5,458,602 - BWI76_RS26370 0.58 +0.7 5,458,614 + BWI76_RS26370 0.59 +0.1 5,458,614 + BWI76_RS26370 0.59 -2.5 5,458,622 - BWI76_RS26370 0.59 +0.4 5,458,680 - BWI76_RS26370 0.64 +0.8 5,458,903 + BWI76_RS26370 0.82 +0.4 5,458,911 - BWI76_RS26370 0.83 +1.4 5,459,069 + -0.5 5,459,077 - +0.3 5,459,314 + -0.6 5,459,385 - +1.1 5,459,523 + BWI76_RS26375 0.19 +0.3 5,459,523 + BWI76_RS26375 0.19 -1.7 5,459,531 - BWI76_RS26375 0.20 +0.8 5,459,531 - BWI76_RS26375 0.20 -0.5 5,459,541 - BWI76_RS26375 0.21 -0.3 5,459,650 + BWI76_RS26375 0.29 +1.6 5,459,669 - BWI76_RS26375 0.31 +0.4 5,459,786 - BWI76_RS26375 0.40 +0.3 5,459,788 + BWI76_RS26375 0.40 +0.5 5,459,835 + BWI76_RS26375 0.44 -1.6 5,460,070 + -1.0 5,460,104 + BWI76_RS26375 0.65 +0.3 5,460,111 + BWI76_RS26375 0.66 +0.6
Or see this region's nucleotide sequence