Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25240 and BWI76_RS25245 are separated by 14 nucleotides BWI76_RS25245 and BWI76_RS25250 overlap by 4 nucleotides BWI76_RS25250 and BWI76_RS25255 overlap by 32 nucleotides
BWI76_RS25240: BWI76_RS25240 - D-arabinose 5-phosphate isomerase, at 5,248,414 to 5,249,400
_RS25240
BWI76_RS25245: BWI76_RS25245 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 5,249,415 to 5,249,981
_RS25245
BWI76_RS25250: BWI76_RS25250 - LPS export ABC transporter periplasmic protein LptC, at 5,249,978 to 5,250,553
_RS25250
BWI76_RS25255: BWI76_RS25255 - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 5,250,522 to 5,251,067
_RS25255
Position (kb) 5249
5250 Strain fitness (log2 ratio) -2
-1
0
1 at 5248.489 kb on + strand at 5248.489 kb on + strand at 5248.694 kb on - strand, within BWI76_RS25240 at 5248.730 kb on - strand, within BWI76_RS25240 at 5248.858 kb on - strand, within BWI76_RS25240 at 5248.882 kb on + strand, within BWI76_RS25240 at 5248.907 kb on - strand, within BWI76_RS25240 at 5248.917 kb on + strand, within BWI76_RS25240 at 5249.215 kb on - strand, within BWI76_RS25240 at 5249.395 kb on + strand at 5249.446 kb on + strand at 5249.595 kb on + strand, within BWI76_RS25245 at 5249.720 kb on + strand, within BWI76_RS25245 at 5249.823 kb on + strand, within BWI76_RS25245 at 5249.945 kb on + strand at 5250.508 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 5,248,489 + +1.1 5,248,489 + +0.2 5,248,694 - BWI76_RS25240 0.28 +0.3 5,248,730 - BWI76_RS25240 0.32 -0.4 5,248,858 - BWI76_RS25240 0.45 +0.3 5,248,882 + BWI76_RS25240 0.47 -0.2 5,248,907 - BWI76_RS25240 0.50 -2.0 5,248,917 + BWI76_RS25240 0.51 -0.2 5,249,215 - BWI76_RS25240 0.81 -2.6 5,249,395 + -1.1 5,249,446 + +1.3 5,249,595 + BWI76_RS25245 0.32 -1.8 5,249,720 + BWI76_RS25245 0.54 -0.1 5,249,823 + BWI76_RS25245 0.72 +0.6 5,249,945 + -0.9 5,250,508 + +0.2
Or see this region's nucleotide sequence