Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23130 and BWI76_RS23135 overlap by 11 nucleotides BWI76_RS23135 and BWI76_RS23140 are separated by 19 nucleotides BWI76_RS23140 and BWI76_RS23145 are separated by 24 nucleotides BWI76_RS23145 and BWI76_RS23150 overlap by 1 nucleotides
BWI76_RS23130: BWI76_RS23130 - diol dehydratase reactivase subunit alpha, at 4,821,597 to 4,823,429
_RS23130
BWI76_RS23135: BWI76_RS23135 - propanediol dehydratase, at 4,823,419 to 4,823,769
_RS23135
BWI76_RS23140: BWI76_RS23140 - carboxysome shell protein, at 4,823,789 to 4,824,064
_RS23140
BWI76_RS23145: BWI76_RS23145 - propanediol utilization protein, at 4,824,089 to 4,824,550
_RS23145
BWI76_RS23150: BWI76_RS23150 - propanediol utilization phosphotransacylase, at 4,824,550 to 4,825,182
_RS23150
Position (kb) 4823
4824 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4822.466 kb on - strand, within BWI76_RS23130 at 4822.533 kb on + strand, within BWI76_RS23130 at 4822.538 kb on + strand at 4822.609 kb on + strand, within BWI76_RS23130 at 4822.646 kb on - strand, within BWI76_RS23130 at 4822.955 kb on - strand, within BWI76_RS23130 at 4823.457 kb on - strand, within BWI76_RS23135 at 4823.491 kb on + strand, within BWI76_RS23135 at 4823.559 kb on + strand, within BWI76_RS23135 at 4823.567 kb on - strand, within BWI76_RS23135 at 4823.914 kb on - strand, within BWI76_RS23140 at 4823.914 kb on - strand, within BWI76_RS23140 at 4824.082 kb on + strand at 4824.675 kb on - strand, within BWI76_RS23150
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 4,822,466 - BWI76_RS23130 0.47 -2.2 4,822,533 + BWI76_RS23130 0.51 +0.6 4,822,538 + -0.4 4,822,609 + BWI76_RS23130 0.55 +0.3 4,822,646 - BWI76_RS23130 0.57 +2.6 4,822,955 - BWI76_RS23130 0.74 +0.1 4,823,457 - BWI76_RS23135 0.11 +0.8 4,823,491 + BWI76_RS23135 0.21 +1.0 4,823,559 + BWI76_RS23135 0.40 +0.6 4,823,567 - BWI76_RS23135 0.42 +0.6 4,823,914 - BWI76_RS23140 0.45 +0.2 4,823,914 - BWI76_RS23140 0.45 +0.2 4,824,082 + +2.8 4,824,675 - BWI76_RS23150 0.20 +1.1
Or see this region's nucleotide sequence