Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23040 and BWI76_RS23045 are separated by 10 nucleotides BWI76_RS23045 and BWI76_RS23050 overlap by 4 nucleotides BWI76_RS23050 and BWI76_RS23055 overlap by 1 nucleotides
BWI76_RS23040: BWI76_RS23040 - sirohydrochlorin cobaltochelatase, at 4,805,250 to 4,806,044
_RS23040
BWI76_RS23045: BWI76_RS23045 - cobalt-precorrin-6A/precorrin-6x reductase, at 4,806,055 to 4,806,834
_RS23045
BWI76_RS23050: BWI76_RS23050 - cobalt-precorrin-3B C(17)-methyltransferase, at 4,806,831 to 4,807,556
_RS23050
BWI76_RS23055: BWI76_RS23055 - cobalamin biosynthesis protein CbiG, at 4,807,556 to 4,808,611
_RS23055
Position (kb) 4806
4807
4808 Strain fitness (log2 ratio) -1
0
1
2 at 4805.863 kb on + strand, within BWI76_RS23040 at 4805.888 kb on + strand, within BWI76_RS23040 at 4806.162 kb on + strand, within BWI76_RS23045 at 4806.304 kb on + strand, within BWI76_RS23045 at 4806.449 kb on + strand, within BWI76_RS23045 at 4806.477 kb on + strand, within BWI76_RS23045 at 4806.494 kb on + strand, within BWI76_RS23045 at 4806.494 kb on + strand, within BWI76_RS23045 at 4806.494 kb on + strand, within BWI76_RS23045 at 4806.494 kb on + strand, within BWI76_RS23045 at 4806.494 kb on + strand, within BWI76_RS23045 at 4806.494 kb on + strand, within BWI76_RS23045 at 4806.523 kb on + strand, within BWI76_RS23045 at 4806.613 kb on - strand, within BWI76_RS23045 at 4806.836 kb on + strand at 4806.846 kb on - strand at 4806.992 kb on - strand, within BWI76_RS23050 at 4807.031 kb on + strand, within BWI76_RS23050 at 4807.031 kb on + strand, within BWI76_RS23050 at 4807.609 kb on - strand at 4807.785 kb on - strand, within BWI76_RS23055 at 4807.839 kb on - strand, within BWI76_RS23055 at 4808.209 kb on + strand, within BWI76_RS23055
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 4,805,863 + BWI76_RS23040 0.77 -0.1 4,805,888 + BWI76_RS23040 0.80 +0.2 4,806,162 + BWI76_RS23045 0.14 +0.6 4,806,304 + BWI76_RS23045 0.32 +0.2 4,806,449 + BWI76_RS23045 0.51 +0.7 4,806,477 + BWI76_RS23045 0.54 +1.1 4,806,494 + BWI76_RS23045 0.56 +0.4 4,806,494 + BWI76_RS23045 0.56 +0.1 4,806,494 + BWI76_RS23045 0.56 +2.0 4,806,494 + BWI76_RS23045 0.56 +0.8 4,806,494 + BWI76_RS23045 0.56 -0.2 4,806,494 + BWI76_RS23045 0.56 +0.5 4,806,523 + BWI76_RS23045 0.60 +1.2 4,806,613 - BWI76_RS23045 0.72 -0.3 4,806,836 + -1.0 4,806,846 - +2.0 4,806,992 - BWI76_RS23050 0.22 +1.9 4,807,031 + BWI76_RS23050 0.28 +0.2 4,807,031 + BWI76_RS23050 0.28 +1.6 4,807,609 - +1.2 4,807,785 - BWI76_RS23055 0.22 -0.2 4,807,839 - BWI76_RS23055 0.27 +0.2 4,808,209 + BWI76_RS23055 0.62 +1.0
Or see this region's nucleotide sequence