Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22925 and BWI76_RS22930 are separated by 93 nucleotides BWI76_RS22930 and BWI76_RS22935 are separated by 1 nucleotides BWI76_RS22935 and BWI76_RS22940 are separated by 57 nucleotides
BWI76_RS22925: BWI76_RS22925 - L-fucose isomerase, at 4,786,672 to 4,788,447
_RS22925
BWI76_RS22930: BWI76_RS22930 - L-fuculokinase (EC 2.7.1.51) <small>(from data)</small>, at 4,788,541 to 4,789,959
_RS22930
BWI76_RS22935: BWI76_RS22935 - L-fucose mutarotase, at 4,789,961 to 4,790,383
_RS22935
BWI76_RS22940: BWI76_RS22940 - DNA-binding transcriptional activator FucR, at 4,790,441 to 4,791,151
_RS22940
Position (kb) 4788
4789
4790 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4787.633 kb on - strand, within BWI76_RS22925 at 4787.699 kb on - strand, within BWI76_RS22925 at 4787.737 kb on + strand, within BWI76_RS22925 at 4788.026 kb on - strand, within BWI76_RS22925 at 4788.179 kb on + strand, within BWI76_RS22925 at 4788.284 kb on - strand at 4788.300 kb on + strand at 4788.343 kb on - strand at 4788.570 kb on - strand at 4788.745 kb on + strand, within BWI76_RS22930 at 4788.775 kb on + strand, within BWI76_RS22930 at 4789.289 kb on - strand, within BWI76_RS22930 at 4789.302 kb on + strand, within BWI76_RS22930 at 4789.490 kb on - strand, within BWI76_RS22930 at 4789.680 kb on + strand, within BWI76_RS22930 at 4789.944 kb on + strand at 4790.064 kb on - strand, within BWI76_RS22935 at 4790.311 kb on - strand, within BWI76_RS22935 at 4790.564 kb on + strand, within BWI76_RS22940 at 4790.920 kb on + strand, within BWI76_RS22940
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 4,787,633 - BWI76_RS22925 0.54 +0.3 4,787,699 - BWI76_RS22925 0.58 +0.9 4,787,737 + BWI76_RS22925 0.60 -0.1 4,788,026 - BWI76_RS22925 0.76 -2.1 4,788,179 + BWI76_RS22925 0.85 +0.8 4,788,284 - +0.5 4,788,300 + -0.6 4,788,343 - -2.0 4,788,570 - -2.3 4,788,745 + BWI76_RS22930 0.14 +0.4 4,788,775 + BWI76_RS22930 0.16 +0.5 4,789,289 - BWI76_RS22930 0.53 +0.3 4,789,302 + BWI76_RS22930 0.54 -1.4 4,789,490 - BWI76_RS22930 0.67 +1.7 4,789,680 + BWI76_RS22930 0.80 +2.2 4,789,944 + +0.2 4,790,064 - BWI76_RS22935 0.24 -2.6 4,790,311 - BWI76_RS22935 0.83 -3.5 4,790,564 + BWI76_RS22940 0.17 +0.4 4,790,920 + BWI76_RS22940 0.67 -1.3
Or see this region's nucleotide sequence