Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22765 and BWI76_RS22770 are separated by 346 nucleotides BWI76_RS22770 and BWI76_RS22775 overlap by 72 nucleotides BWI76_RS22775 and BWI76_RS22780 are separated by 56 nucleotides
BWI76_RS22765: BWI76_RS22765 - sulfite reductase subunit alpha, at 4,745,693 to 4,747,492
_RS22765
BWI76_RS22770: BWI76_RS22770 - 6-pyruvoyl tetrahydrobiopterin synthase, at 4,747,839 to 4,748,000
_RS22770
BWI76_RS22775: BWI76_RS22775 - 6-carboxytetrahydropterin synthase QueD, at 4,747,929 to 4,748,291
_RS22775
BWI76_RS22780: BWI76_RS22780 - 7-carboxy-7-deazaguanine synthase QueE, at 4,748,348 to 4,749,019
_RS22780
Position (kb) 4747
4748
4749 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4747.131 kb on - strand, within BWI76_RS22765 at 4747.134 kb on - strand, within BWI76_RS22765 at 4747.136 kb on - strand, within BWI76_RS22765 at 4747.136 kb on - strand, within BWI76_RS22765 at 4747.146 kb on - strand, within BWI76_RS22765 at 4747.218 kb on - strand, within BWI76_RS22765 at 4747.324 kb on + strand at 4747.332 kb on - strand at 4747.376 kb on - strand at 4747.376 kb on - strand at 4747.498 kb on + strand at 4747.519 kb on - strand at 4747.612 kb on + strand at 4747.612 kb on + strand at 4747.612 kb on + strand at 4747.613 kb on - strand at 4747.620 kb on - strand at 4747.620 kb on - strand at 4747.620 kb on - strand at 4747.620 kb on - strand at 4747.677 kb on + strand at 4747.692 kb on - strand at 4747.943 kb on + strand at 4747.943 kb on + strand at 4747.943 kb on + strand at 4747.957 kb on + strand at 4747.968 kb on + strand at 4748.026 kb on + strand, within BWI76_RS22775 at 4748.096 kb on + strand, within BWI76_RS22775 at 4748.096 kb on + strand, within BWI76_RS22775 at 4748.165 kb on + strand, within BWI76_RS22775 at 4748.187 kb on - strand, within BWI76_RS22775 at 4748.330 kb on - strand at 4748.401 kb on - strand at 4748.468 kb on - strand, within BWI76_RS22780 at 4748.605 kb on + strand, within BWI76_RS22780 at 4748.613 kb on - strand, within BWI76_RS22780 at 4748.627 kb on + strand, within BWI76_RS22780 at 4748.635 kb on - strand, within BWI76_RS22780 at 4748.635 kb on - strand, within BWI76_RS22780 at 4748.774 kb on - strand, within BWI76_RS22780 at 4748.779 kb on - strand, within BWI76_RS22780 at 4748.833 kb on + strand, within BWI76_RS22780 at 4748.884 kb on + strand, within BWI76_RS22780 at 4748.972 kb on + strand at 4748.974 kb on - strand at 4748.987 kb on + strand at 4748.988 kb on + strand at 4748.996 kb on - strand at 4749.014 kb on - strand at 4749.030 kb on + strand at 4749.068 kb on + strand at 4749.073 kb on + strand at 4749.073 kb on + strand at 4749.079 kb on + strand at 4749.081 kb on - strand at 4749.082 kb on - strand at 4749.084 kb on + strand at 4749.118 kb on + strand at 4749.126 kb on - strand at 4749.128 kb on + strand at 4749.134 kb on - strand at 4749.134 kb on - strand at 4749.156 kb on - strand at 4749.196 kb on + strand at 4749.204 kb on - strand at 4749.204 kb on - strand at 4749.238 kb on + strand at 4749.238 kb on + strand at 4749.238 kb on + strand at 4749.246 kb on - strand at 4749.246 kb on - strand at 4749.246 kb on - strand at 4749.246 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 4,747,131 - BWI76_RS22765 0.80 +1.2 4,747,134 - BWI76_RS22765 0.80 -2.0 4,747,136 - BWI76_RS22765 0.80 -3.3 4,747,136 - BWI76_RS22765 0.80 -1.4 4,747,146 - BWI76_RS22765 0.81 -0.4 4,747,218 - BWI76_RS22765 0.85 -1.4 4,747,324 + -1.9 4,747,332 - +0.2 4,747,376 - -1.7 4,747,376 - -0.2 4,747,498 + +1.2 4,747,519 - +0.3 4,747,612 + +0.2 4,747,612 + +1.2 4,747,612 + -2.4 4,747,613 - -0.1 4,747,620 - -2.7 4,747,620 - -1.6 4,747,620 - -2.4 4,747,620 - -0.6 4,747,677 + -0.8 4,747,692 - +0.1 4,747,943 + +1.6 4,747,943 + -0.9 4,747,943 + +0.4 4,747,957 + +0.7 4,747,968 + -0.3 4,748,026 + BWI76_RS22775 0.27 +0.4 4,748,096 + BWI76_RS22775 0.46 -0.4 4,748,096 + BWI76_RS22775 0.46 +0.2 4,748,165 + BWI76_RS22775 0.65 +0.5 4,748,187 - BWI76_RS22775 0.71 +2.7 4,748,330 - -2.3 4,748,401 - +0.9 4,748,468 - BWI76_RS22780 0.18 -0.1 4,748,605 + BWI76_RS22780 0.38 -0.9 4,748,613 - BWI76_RS22780 0.39 +1.1 4,748,627 + BWI76_RS22780 0.42 -1.6 4,748,635 - BWI76_RS22780 0.43 +0.1 4,748,635 - BWI76_RS22780 0.43 +0.5 4,748,774 - BWI76_RS22780 0.63 +0.5 4,748,779 - BWI76_RS22780 0.64 +0.3 4,748,833 + BWI76_RS22780 0.72 +1.2 4,748,884 + BWI76_RS22780 0.80 +0.4 4,748,972 + -2.0 4,748,974 - -1.4 4,748,987 + -0.4 4,748,988 + +0.6 4,748,996 - -1.2 4,749,014 - -0.4 4,749,030 + -0.4 4,749,068 + -0.1 4,749,073 + -1.4 4,749,073 + -0.9 4,749,079 + +0.2 4,749,081 - -0.6 4,749,082 - +0.7 4,749,084 + +0.2 4,749,118 + -1.7 4,749,126 - +0.2 4,749,128 + +0.3 4,749,134 - -2.1 4,749,134 - -1.1 4,749,156 - -2.4 4,749,196 + +1.2 4,749,204 - -0.8 4,749,204 - -1.0 4,749,238 + -1.7 4,749,238 + -1.3 4,749,238 + +1.5 4,749,246 - -2.3 4,749,246 - +0.5 4,749,246 - -0.4 4,749,246 - +2.8
Or see this region's nucleotide sequence