Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21750 and BWI76_RS21755 are separated by 14 nucleotides BWI76_RS21755 and BWI76_RS21760 are separated by 100 nucleotides
BWI76_RS21750: BWI76_RS21750 - amidohydrolase family protein, at 4,545,979 to 4,547,376
_RS21750
BWI76_RS21755: BWI76_RS21755 - hypothetical protein, at 4,547,391 to 4,547,864
_RS21755
BWI76_RS21760: BWI76_RS21760 - SMP-30/gluconolactonase/LRE family protein, at 4,547,965 to 4,548,825
_RS21760
Position (kb) 4547
4548 Strain fitness (log2 ratio) -2
-1
0
1 at 4546.697 kb on + strand, within BWI76_RS21750 at 4546.736 kb on + strand, within BWI76_RS21750 at 4546.744 kb on - strand, within BWI76_RS21750 at 4546.781 kb on + strand, within BWI76_RS21750 at 4546.801 kb on - strand, within BWI76_RS21750 at 4546.913 kb on + strand, within BWI76_RS21750 at 4547.043 kb on + strand, within BWI76_RS21750 at 4547.050 kb on + strand, within BWI76_RS21750 at 4547.119 kb on - strand, within BWI76_RS21750 at 4547.248 kb on + strand at 4547.256 kb on - strand at 4547.434 kb on + strand at 4547.451 kb on + strand, within BWI76_RS21755 at 4547.520 kb on + strand, within BWI76_RS21755 at 4547.570 kb on - strand, within BWI76_RS21755 at 4547.645 kb on - strand, within BWI76_RS21755 at 4547.747 kb on + strand, within BWI76_RS21755 at 4547.760 kb on - strand, within BWI76_RS21755 at 4547.926 kb on - strand at 4548.139 kb on - strand, within BWI76_RS21760 at 4548.267 kb on - strand, within BWI76_RS21760 at 4548.386 kb on + strand, within BWI76_RS21760 at 4548.439 kb on + strand, within BWI76_RS21760 at 4548.489 kb on - strand, within BWI76_RS21760 at 4548.564 kb on + strand, within BWI76_RS21760 at 4548.637 kb on + strand, within BWI76_RS21760 at 4548.655 kb on + strand, within BWI76_RS21760 at 4548.710 kb on - strand, within BWI76_RS21760 at 4548.710 kb on - strand, within BWI76_RS21760 at 4548.820 kb on + strand at 4548.828 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 4,546,697 + BWI76_RS21750 0.51 +0.5 4,546,736 + BWI76_RS21750 0.54 -0.3 4,546,744 - BWI76_RS21750 0.55 +0.1 4,546,781 + BWI76_RS21750 0.57 -1.1 4,546,801 - BWI76_RS21750 0.59 -2.2 4,546,913 + BWI76_RS21750 0.67 +1.3 4,547,043 + BWI76_RS21750 0.76 -0.1 4,547,050 + BWI76_RS21750 0.77 +0.1 4,547,119 - BWI76_RS21750 0.82 +0.3 4,547,248 + -0.3 4,547,256 - -1.0 4,547,434 + -0.5 4,547,451 + BWI76_RS21755 0.13 +0.5 4,547,520 + BWI76_RS21755 0.27 +0.6 4,547,570 - BWI76_RS21755 0.38 -1.3 4,547,645 - BWI76_RS21755 0.54 +1.0 4,547,747 + BWI76_RS21755 0.75 +0.5 4,547,760 - BWI76_RS21755 0.78 +1.0 4,547,926 - +1.7 4,548,139 - BWI76_RS21760 0.20 -0.5 4,548,267 - BWI76_RS21760 0.35 +0.7 4,548,386 + BWI76_RS21760 0.49 +1.3 4,548,439 + BWI76_RS21760 0.55 +0.3 4,548,489 - BWI76_RS21760 0.61 -1.3 4,548,564 + BWI76_RS21760 0.70 -1.2 4,548,637 + BWI76_RS21760 0.78 +0.3 4,548,655 + BWI76_RS21760 0.80 +0.4 4,548,710 - BWI76_RS21760 0.87 +0.4 4,548,710 - BWI76_RS21760 0.87 +0.7 4,548,820 + +1.4 4,548,828 - -1.5
Or see this region's nucleotide sequence