Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20675

Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20670 and BWI76_RS20675 are separated by 14 nucleotidesBWI76_RS20675 and BWI76_RS20680 are separated by 45 nucleotidesBWI76_RS20680 and BWI76_RS20685 overlap by 4 nucleotides BWI76_RS20670: BWI76_RS20670 - transcriptional regulator PtsJ, at 4,324,370 to 4,325,668 _RS20670 BWI76_RS20675: BWI76_RS20675 - GMP synthase, at 4,325,683 to 4,326,390 _RS20675 BWI76_RS20680: BWI76_RS20680 - hypothetical protein, at 4,326,436 to 4,326,840 _RS20680 BWI76_RS20685: BWI76_RS20685 - cysteine synthase B, at 4,326,837 to 4,327,748 _RS20685 Position (kb) 4325 4326 4327Strain fitness (log2 ratio) -1 0 1 2at 4325.054 kb on - strand, within BWI76_RS20670at 4325.140 kb on + strand, within BWI76_RS20670at 4325.549 kb on + strandat 4325.701 kb on - strandat 4325.726 kb on + strandat 4325.731 kb on + strandat 4325.779 kb on - strand, within BWI76_RS20675at 4325.864 kb on + strand, within BWI76_RS20675at 4325.904 kb on + strand, within BWI76_RS20675at 4326.249 kb on + strand, within BWI76_RS20675at 4326.249 kb on + strandat 4326.258 kb on - strand, within BWI76_RS20675at 4326.315 kb on + strand, within BWI76_RS20675at 4327.165 kb on - strand, within BWI76_RS20685

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
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4,325,054 - BWI76_RS20670 0.53 -0.2
4,325,140 + BWI76_RS20670 0.59 +0.6
4,325,549 + +0.2
4,325,701 - +0.6
4,325,726 + -1.2
4,325,731 + +0.6
4,325,779 - BWI76_RS20675 0.14 +1.0
4,325,864 + BWI76_RS20675 0.26 +1.2
4,325,904 + BWI76_RS20675 0.31 +0.8
4,326,249 + BWI76_RS20675 0.80 -0.0
4,326,249 + -0.8
4,326,258 - BWI76_RS20675 0.81 +0.2
4,326,315 + BWI76_RS20675 0.89 +2.4
4,327,165 - BWI76_RS20685 0.36 +1.0

Or see this region's nucleotide sequence