Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20595 and BWI76_RS20600 are separated by 96 nucleotides BWI76_RS20600 and BWI76_RS20605 are separated by 60 nucleotides
BWI76_RS20595: BWI76_RS20595 - LysR family transcriptional regulator, at 4,309,486 to 4,310,370
_RS20595
BWI76_RS20600: BWI76_RS20600 - nucleoside permease, at 4,310,467 to 4,311,723
_RS20600
BWI76_RS20605: BWI76_RS20605 - purine-nucleoside phosphorylase, at 4,311,784 to 4,312,617
_RS20605
Position (kb) 4310
4311
4312 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4309.539 kb on + strand at 4309.766 kb on - strand, within BWI76_RS20595 at 4309.881 kb on - strand, within BWI76_RS20595 at 4309.887 kb on + strand at 4310.179 kb on + strand, within BWI76_RS20595 at 4310.179 kb on + strand, within BWI76_RS20595 at 4310.179 kb on + strand, within BWI76_RS20595 at 4310.179 kb on + strand, within BWI76_RS20595 at 4310.179 kb on + strand, within BWI76_RS20595 at 4310.187 kb on - strand, within BWI76_RS20595 at 4310.187 kb on - strand, within BWI76_RS20595 at 4310.272 kb on - strand, within BWI76_RS20595 at 4310.394 kb on + strand at 4310.414 kb on + strand at 4310.660 kb on + strand, within BWI76_RS20600 at 4310.775 kb on - strand, within BWI76_RS20600 at 4310.796 kb on + strand, within BWI76_RS20600 at 4310.804 kb on - strand, within BWI76_RS20600 at 4310.811 kb on - strand, within BWI76_RS20600 at 4310.842 kb on + strand, within BWI76_RS20600 at 4310.849 kb on + strand, within BWI76_RS20600 at 4311.241 kb on - strand, within BWI76_RS20600 at 4311.241 kb on - strand, within BWI76_RS20600 at 4311.255 kb on + strand, within BWI76_RS20600 at 4311.263 kb on - strand, within BWI76_RS20600 at 4311.371 kb on - strand, within BWI76_RS20600 at 4311.498 kb on - strand, within BWI76_RS20600 at 4311.500 kb on - strand, within BWI76_RS20600 at 4311.512 kb on - strand, within BWI76_RS20600 at 4311.514 kb on - strand, within BWI76_RS20600 at 4311.524 kb on - strand, within BWI76_RS20600 at 4311.640 kb on + strand at 4311.811 kb on + strand at 4311.927 kb on + strand, within BWI76_RS20605 at 4312.190 kb on - strand, within BWI76_RS20605 at 4312.320 kb on + strand, within BWI76_RS20605 at 4312.378 kb on + strand, within BWI76_RS20605 at 4312.577 kb on + strand at 4312.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 4,309,539 + +1.0 4,309,766 - BWI76_RS20595 0.32 +0.2 4,309,881 - BWI76_RS20595 0.45 -3.2 4,309,887 + +1.2 4,310,179 + BWI76_RS20595 0.78 -1.1 4,310,179 + BWI76_RS20595 0.78 +0.2 4,310,179 + BWI76_RS20595 0.78 -1.5 4,310,179 + BWI76_RS20595 0.78 +0.7 4,310,179 + BWI76_RS20595 0.78 +0.7 4,310,187 - BWI76_RS20595 0.79 +0.4 4,310,187 - BWI76_RS20595 0.79 +0.2 4,310,272 - BWI76_RS20595 0.89 +0.6 4,310,394 + -0.1 4,310,414 + +1.2 4,310,660 + BWI76_RS20600 0.15 -0.8 4,310,775 - BWI76_RS20600 0.25 +0.1 4,310,796 + BWI76_RS20600 0.26 +2.2 4,310,804 - BWI76_RS20600 0.27 +0.3 4,310,811 - BWI76_RS20600 0.27 +0.2 4,310,842 + BWI76_RS20600 0.30 +1.2 4,310,849 + BWI76_RS20600 0.30 +0.3 4,311,241 - BWI76_RS20600 0.62 +0.1 4,311,241 - BWI76_RS20600 0.62 -1.5 4,311,255 + BWI76_RS20600 0.63 +0.4 4,311,263 - BWI76_RS20600 0.63 -3.6 4,311,371 - BWI76_RS20600 0.72 +0.2 4,311,498 - BWI76_RS20600 0.82 +0.8 4,311,500 - BWI76_RS20600 0.82 +0.5 4,311,512 - BWI76_RS20600 0.83 +0.4 4,311,514 - BWI76_RS20600 0.83 +2.2 4,311,524 - BWI76_RS20600 0.84 +0.9 4,311,640 + -0.1 4,311,811 + -1.4 4,311,927 + BWI76_RS20605 0.17 +0.3 4,312,190 - BWI76_RS20605 0.49 +0.7 4,312,320 + BWI76_RS20605 0.64 -0.8 4,312,378 + BWI76_RS20605 0.71 +0.6 4,312,577 + +0.8 4,312,621 - +2.2
Or see this region's nucleotide sequence