Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20015 and BWI76_RS20020 are separated by 137 nucleotides BWI76_RS20020 and BWI76_RS20025 overlap by 9 nucleotides BWI76_RS20025 and BWI76_RS20030 overlap by 14 nucleotides BWI76_RS20030 and BWI76_RS20035 are separated by 134 nucleotides
BWI76_RS20015: BWI76_RS20015 - hypothetical protein, at 4,193,769 to 4,194,785
_RS20015
BWI76_RS20020: BWI76_RS20020 - nucleoside triphosphatase NudI, at 4,194,923 to 4,195,348
_RS20020
BWI76_RS20025: BWI76_RS20025 - HAMP domain-containing histidine kinase, at 4,195,340 to 4,196,755
_RS20025
BWI76_RS20030: BWI76_RS20030 - DNA-binding response regulator, at 4,196,742 to 4,197,416
_RS20030
BWI76_RS20035: BWI76_RS20035 - hypothetical protein, at 4,197,551 to 4,198,531
_RS20035
Position (kb) 4195
4196
4197 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4194.512 kb on + strand, within BWI76_RS20015 at 4194.824 kb on - strand at 4195.091 kb on + strand, within BWI76_RS20020 at 4195.283 kb on + strand, within BWI76_RS20020 at 4195.314 kb on - strand at 4195.956 kb on + strand, within BWI76_RS20025 at 4196.001 kb on + strand, within BWI76_RS20025 at 4196.305 kb on - strand, within BWI76_RS20025 at 4196.528 kb on + strand, within BWI76_RS20025 at 4196.762 kb on + strand at 4196.811 kb on + strand, within BWI76_RS20030 at 4196.846 kb on + strand, within BWI76_RS20030 at 4197.056 kb on - strand, within BWI76_RS20030 at 4197.156 kb on - strand, within BWI76_RS20030 at 4197.233 kb on + strand, within BWI76_RS20030 at 4197.527 kb on + strand at 4197.603 kb on - strand at 4197.610 kb on + strand at 4197.613 kb on - strand at 4197.705 kb on + strand, within BWI76_RS20035
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 4,194,512 + BWI76_RS20015 0.73 -0.9 4,194,824 - -0.5 4,195,091 + BWI76_RS20020 0.39 +0.5 4,195,283 + BWI76_RS20020 0.85 -0.0 4,195,314 - -1.5 4,195,956 + BWI76_RS20025 0.44 +0.8 4,196,001 + BWI76_RS20025 0.47 +1.1 4,196,305 - BWI76_RS20025 0.68 +0.2 4,196,528 + BWI76_RS20025 0.84 +1.0 4,196,762 + -0.4 4,196,811 + BWI76_RS20030 0.10 -0.6 4,196,846 + BWI76_RS20030 0.15 -3.2 4,197,056 - BWI76_RS20030 0.47 -0.9 4,197,156 - BWI76_RS20030 0.61 +0.4 4,197,233 + BWI76_RS20030 0.73 +0.5 4,197,527 + +0.7 4,197,603 - -0.1 4,197,610 + +0.6 4,197,613 - -1.7 4,197,705 + BWI76_RS20035 0.16 +1.4
Or see this region's nucleotide sequence