Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19910 and BWI76_RS19915 are separated by 16 nucleotides BWI76_RS19915 and BWI76_RS19920 are separated by 43 nucleotides BWI76_RS19920 and BWI76_RS19925 are separated by 131 nucleotides
BWI76_RS19910: BWI76_RS19910 - phosphotransferase RcsD, at 4,164,945 to 4,167,605
_RS19910
BWI76_RS19915: BWI76_RS19915 - DNA-binding response regulator, at 4,167,622 to 4,168,272
_RS19915
BWI76_RS19920: BWI76_RS19920 - hybrid sensor histidine kinase/response regulator, at 4,168,316 to 4,171,162
_RS19920
BWI76_RS19925: BWI76_RS19925 - DNA gyrase subunit A, at 4,171,294 to 4,173,927
_RS19925
Position (kb) 4168
4169
4170
4171
4172 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4167.377 kb on - strand at 4167.412 kb on - strand at 4167.574 kb on + strand at 4167.595 kb on - strand at 4167.654 kb on + strand at 4168.101 kb on - strand, within BWI76_RS19915 at 4168.214 kb on + strand at 4168.313 kb on + strand at 4168.504 kb on - strand at 4168.804 kb on + strand, within BWI76_RS19920 at 4168.890 kb on + strand, within BWI76_RS19920 at 4168.993 kb on + strand, within BWI76_RS19920 at 4169.341 kb on + strand, within BWI76_RS19920 at 4169.349 kb on - strand, within BWI76_RS19920 at 4169.471 kb on - strand, within BWI76_RS19920 at 4169.550 kb on + strand, within BWI76_RS19920 at 4169.624 kb on + strand, within BWI76_RS19920 at 4169.645 kb on - strand, within BWI76_RS19920 at 4169.663 kb on - strand, within BWI76_RS19920 at 4169.778 kb on - strand, within BWI76_RS19920 at 4169.893 kb on + strand, within BWI76_RS19920 at 4170.277 kb on - strand, within BWI76_RS19920 at 4170.510 kb on - strand at 4170.851 kb on - strand, within BWI76_RS19920 at 4171.004 kb on - strand at 4171.198 kb on - strand at 4171.200 kb on + strand at 4171.260 kb on + strand at 4171.268 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 4,167,377 - -2.4 4,167,412 - -3.8 4,167,574 + -2.8 4,167,595 - -2.6 4,167,654 + -2.2 4,168,101 - BWI76_RS19915 0.74 -2.6 4,168,214 + -3.4 4,168,313 + -0.0 4,168,504 - -3.5 4,168,804 + BWI76_RS19920 0.17 +1.1 4,168,890 + BWI76_RS19920 0.20 -3.2 4,168,993 + BWI76_RS19920 0.24 -2.2 4,169,341 + BWI76_RS19920 0.36 -2.4 4,169,349 - BWI76_RS19920 0.36 -3.9 4,169,471 - BWI76_RS19920 0.41 -1.5 4,169,550 + BWI76_RS19920 0.43 +0.1 4,169,624 + BWI76_RS19920 0.46 -1.1 4,169,645 - BWI76_RS19920 0.47 -2.6 4,169,663 - BWI76_RS19920 0.47 -2.1 4,169,778 - BWI76_RS19920 0.51 -2.5 4,169,893 + BWI76_RS19920 0.55 -1.5 4,170,277 - BWI76_RS19920 0.69 -1.1 4,170,510 - -1.7 4,170,851 - BWI76_RS19920 0.89 -3.4 4,171,004 - -3.4 4,171,198 - +2.1 4,171,200 + -1.8 4,171,260 + +0.1 4,171,268 - +0.1
Or see this region's nucleotide sequence