Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19880 and BWI76_RS19885 are separated by 168 nucleotides BWI76_RS19885 and BWI76_RS19890 are separated by 76 nucleotides BWI76_RS19890 and BWI76_RS19895 overlap by 1 nucleotides
BWI76_RS19880: BWI76_RS19880 - magnesium transporter, at 4,156,788 to 4,158,224
_RS19880
BWI76_RS19885: BWI76_RS19885 - multidrug ABC transporter permease/ATP-binding protein, at 4,158,393 to 4,160,036
_RS19885
BWI76_RS19890: BWI76_RS19890 - alpha-ketoglutarate-dependent dioxygenase AlkB, at 4,160,113 to 4,160,763
_RS19890
BWI76_RS19895: BWI76_RS19895 - bifunctional transcriptional regulator/O6-methylguanine-DNA methyltransferase, at 4,160,763 to 4,161,827
_RS19895
Position (kb) 4158
4159
4160
4161 Strain fitness (log2 ratio) -2
-1
0
1 at 4157.562 kb on - strand, within BWI76_RS19880 at 4157.627 kb on - strand, within BWI76_RS19880 at 4157.835 kb on + strand, within BWI76_RS19880 at 4158.989 kb on - strand, within BWI76_RS19885 at 4159.072 kb on - strand, within BWI76_RS19885 at 4159.167 kb on + strand, within BWI76_RS19885 at 4159.190 kb on - strand, within BWI76_RS19885 at 4159.909 kb on + strand at 4159.943 kb on + strand at 4159.951 kb on - strand at 4159.951 kb on - strand at 4159.951 kb on - strand at 4159.963 kb on - strand at 4160.078 kb on - strand at 4160.201 kb on - strand, within BWI76_RS19890 at 4160.349 kb on + strand, within BWI76_RS19890 at 4160.604 kb on - strand, within BWI76_RS19890 at 4160.724 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 4,157,562 - BWI76_RS19880 0.54 +0.5 4,157,627 - BWI76_RS19880 0.58 -0.8 4,157,835 + BWI76_RS19880 0.73 -0.1 4,158,989 - BWI76_RS19885 0.36 -0.1 4,159,072 - BWI76_RS19885 0.41 -1.4 4,159,167 + BWI76_RS19885 0.47 -1.9 4,159,190 - BWI76_RS19885 0.48 -0.8 4,159,909 + +0.7 4,159,943 + +0.2 4,159,951 - +0.6 4,159,951 - +0.4 4,159,951 - -0.4 4,159,963 - -0.2 4,160,078 - -0.7 4,160,201 - BWI76_RS19890 0.14 -1.1 4,160,349 + BWI76_RS19890 0.36 +0.8 4,160,604 - BWI76_RS19890 0.75 +0.8 4,160,724 + +0.7
Or see this region's nucleotide sequence