Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17530

Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17520 and BWI76_RS17525 are separated by 48 nucleotidesBWI76_RS17525 and BWI76_RS17530 overlap by 96 nucleotidesBWI76_RS17530 and BWI76_RS17535 are separated by 251 nucleotides BWI76_RS17520: BWI76_RS17520 - carbamate kinase, at 3,633,842 to 3,634,774 _RS17520 BWI76_RS17525: BWI76_RS17525 - XshC-Cox1-family protein, at 3,634,823 to 3,635,656 _RS17525 BWI76_RS17530: BWI76_RS17530 - hypothetical protein, at 3,635,561 to 3,635,938 _RS17530 BWI76_RS17535: BWI76_RS17535 - rRNA metabolism protein, at 3,636,190 to 3,639,294 _RS17535 Position (kb) 3635 3636Strain fitness (log2 ratio) -3 -2 -1 0 1at 3634.782 kb on - strandat 3635.433 kb on - strand, within BWI76_RS17525at 3635.549 kb on + strand, within BWI76_RS17525at 3635.563 kb on + strandat 3635.571 kb on - strandat 3635.582 kb on + strandat 3635.590 kb on - strandat 3635.603 kb on + strandat 3635.613 kb on - strandat 3635.615 kb on + strandat 3635.623 kb on - strandat 3635.623 kb on - strandat 3635.623 kb on - strandat 3635.623 kb on - strandat 3635.631 kb on - strandat 3635.670 kb on - strand, within BWI76_RS17530at 3635.671 kb on - strand, within BWI76_RS17530at 3635.685 kb on + strand, within BWI76_RS17530at 3635.765 kb on + strand, within BWI76_RS17530at 3635.853 kb on + strand, within BWI76_RS17530at 3635.884 kb on + strand, within BWI76_RS17530at 3635.974 kb on + strandat 3635.982 kb on - strandat 3635.982 kb on - strandat 3636.000 kb on + strandat 3636.051 kb on + strandat 3636.059 kb on - strandat 3636.133 kb on + strandat 3636.147 kb on - strandat 3636.173 kb on + strandat 3636.175 kb on + strandat 3636.186 kb on + strandat 3636.249 kb on + strandat 3636.249 kb on + strandat 3636.249 kb on + strandat 3636.257 kb on - strandat 3636.331 kb on + strandat 3636.344 kb on - strandat 3636.349 kb on - strandat 3636.393 kb on - strandat 3636.432 kb on + strandat 3636.440 kb on - strandat 3636.474 kb on + strandat 3636.501 kb on + strand, within BWI76_RS17535at 3636.555 kb on + strand, within BWI76_RS17535at 3636.563 kb on - strand, within BWI76_RS17535at 3636.587 kb on + strand, within BWI76_RS17535at 3636.599 kb on - strand, within BWI76_RS17535at 3636.599 kb on - strand, within BWI76_RS17535at 3636.665 kb on + strand, within BWI76_RS17535at 3636.665 kb on + strand, within BWI76_RS17535at 3636.713 kb on + strand, within BWI76_RS17535at 3636.747 kb on - strand, within BWI76_RS17535at 3636.832 kb on - strand, within BWI76_RS17535at 3636.852 kb on + strand, within BWI76_RS17535at 3636.860 kb on - strand, within BWI76_RS17535

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
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3,634,782 - +0.9
3,635,433 - BWI76_RS17525 0.73 +1.3
3,635,549 + BWI76_RS17525 0.87 +0.7
3,635,563 + +0.5
3,635,571 - -3.2
3,635,582 + -0.3
3,635,590 - +1.6
3,635,603 + +0.0
3,635,613 - +1.4
3,635,615 + -2.0
3,635,623 - -1.0
3,635,623 - +0.1
3,635,623 - +0.0
3,635,623 - -1.1
3,635,631 - -1.3
3,635,670 - BWI76_RS17530 0.29 -0.8
3,635,671 - BWI76_RS17530 0.29 +0.0
3,635,685 + BWI76_RS17530 0.33 -0.6
3,635,765 + BWI76_RS17530 0.54 -1.0
3,635,853 + BWI76_RS17530 0.77 -1.1
3,635,884 + BWI76_RS17530 0.85 +0.0
3,635,974 + +0.0
3,635,982 - -0.1
3,635,982 - +0.6
3,636,000 + -2.5
3,636,051 + -1.2
3,636,059 - +0.9
3,636,133 + -0.3
3,636,147 - -1.1
3,636,173 + -1.5
3,636,175 + +0.3
3,636,186 + +1.3
3,636,249 + -0.6
3,636,249 + +0.0
3,636,249 + -1.8
3,636,257 - -1.0
3,636,331 + -2.6
3,636,344 - -0.3
3,636,349 - -1.3
3,636,393 - -2.3
3,636,432 + +1.5
3,636,440 - +1.2
3,636,474 + +0.0
3,636,501 + BWI76_RS17535 0.10 -2.0
3,636,555 + BWI76_RS17535 0.12 -1.4
3,636,563 - BWI76_RS17535 0.12 +0.1
3,636,587 + BWI76_RS17535 0.13 -2.3
3,636,599 - BWI76_RS17535 0.13 +0.6
3,636,599 - BWI76_RS17535 0.13 +0.4
3,636,665 + BWI76_RS17535 0.15 -0.7
3,636,665 + BWI76_RS17535 0.15 -1.6
3,636,713 + BWI76_RS17535 0.17 -0.6
3,636,747 - BWI76_RS17535 0.18 +1.2
3,636,832 - BWI76_RS17535 0.21 +0.1
3,636,852 + BWI76_RS17535 0.21 +0.4
3,636,860 - BWI76_RS17535 0.22 +0.7

Or see this region's nucleotide sequence