Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17520 and BWI76_RS17525 are separated by 48 nucleotides BWI76_RS17525 and BWI76_RS17530 overlap by 96 nucleotides BWI76_RS17530 and BWI76_RS17535 are separated by 251 nucleotides
BWI76_RS17520: BWI76_RS17520 - carbamate kinase, at 3,633,842 to 3,634,774
_RS17520
BWI76_RS17525: BWI76_RS17525 - XshC-Cox1-family protein, at 3,634,823 to 3,635,656
_RS17525
BWI76_RS17530: BWI76_RS17530 - hypothetical protein, at 3,635,561 to 3,635,938
_RS17530
BWI76_RS17535: BWI76_RS17535 - rRNA metabolism protein, at 3,636,190 to 3,639,294
_RS17535
Position (kb)
3635
3636 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3634.782 kb on - strand at 3635.433 kb on - strand, within BWI76_RS17525 at 3635.549 kb on + strand, within BWI76_RS17525 at 3635.563 kb on + strand at 3635.571 kb on - strand at 3635.582 kb on + strand at 3635.590 kb on - strand at 3635.603 kb on + strand at 3635.613 kb on - strand at 3635.615 kb on + strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.623 kb on - strand at 3635.631 kb on - strand at 3635.670 kb on - strand, within BWI76_RS17530 at 3635.671 kb on - strand, within BWI76_RS17530 at 3635.685 kb on + strand, within BWI76_RS17530 at 3635.765 kb on + strand, within BWI76_RS17530 at 3635.853 kb on + strand, within BWI76_RS17530 at 3635.884 kb on + strand, within BWI76_RS17530 at 3635.974 kb on + strand at 3635.982 kb on - strand at 3635.982 kb on - strand at 3636.000 kb on + strand at 3636.051 kb on + strand at 3636.059 kb on - strand at 3636.133 kb on + strand at 3636.147 kb on - strand at 3636.173 kb on + strand at 3636.175 kb on + strand at 3636.186 kb on + strand at 3636.249 kb on + strand at 3636.249 kb on + strand at 3636.249 kb on + strand at 3636.257 kb on - strand at 3636.331 kb on + strand at 3636.344 kb on - strand at 3636.349 kb on - strand at 3636.393 kb on - strand at 3636.432 kb on + strand at 3636.440 kb on - strand at 3636.474 kb on + strand at 3636.501 kb on + strand, within BWI76_RS17535 at 3636.555 kb on + strand, within BWI76_RS17535 at 3636.563 kb on - strand, within BWI76_RS17535 at 3636.587 kb on + strand, within BWI76_RS17535 at 3636.599 kb on - strand, within BWI76_RS17535 at 3636.599 kb on - strand, within BWI76_RS17535 at 3636.665 kb on + strand, within BWI76_RS17535 at 3636.665 kb on + strand, within BWI76_RS17535 at 3636.713 kb on + strand, within BWI76_RS17535 at 3636.747 kb on - strand, within BWI76_RS17535 at 3636.832 kb on - strand, within BWI76_RS17535 at 3636.852 kb on + strand, within BWI76_RS17535 at 3636.860 kb on - strand, within BWI76_RS17535
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 3,634,782 - +0.9 3,635,433 - BWI76_RS17525 0.73 +1.3 3,635,549 + BWI76_RS17525 0.87 +0.7 3,635,563 + +0.5 3,635,571 - -3.2 3,635,582 + -0.3 3,635,590 - +1.6 3,635,603 + +0.0 3,635,613 - +1.4 3,635,615 + -2.0 3,635,623 - -1.0 3,635,623 - +0.1 3,635,623 - +0.0 3,635,623 - -1.1 3,635,631 - -1.3 3,635,670 - BWI76_RS17530 0.29 -0.8 3,635,671 - BWI76_RS17530 0.29 +0.0 3,635,685 + BWI76_RS17530 0.33 -0.6 3,635,765 + BWI76_RS17530 0.54 -1.0 3,635,853 + BWI76_RS17530 0.77 -1.1 3,635,884 + BWI76_RS17530 0.85 +0.0 3,635,974 + +0.0 3,635,982 - -0.1 3,635,982 - +0.6 3,636,000 + -2.5 3,636,051 + -1.2 3,636,059 - +0.9 3,636,133 + -0.3 3,636,147 - -1.1 3,636,173 + -1.5 3,636,175 + +0.3 3,636,186 + +1.3 3,636,249 + -0.6 3,636,249 + +0.0 3,636,249 + -1.8 3,636,257 - -1.0 3,636,331 + -2.6 3,636,344 - -0.3 3,636,349 - -1.3 3,636,393 - -2.3 3,636,432 + +1.5 3,636,440 - +1.2 3,636,474 + +0.0 3,636,501 + BWI76_RS17535 0.10 -2.0 3,636,555 + BWI76_RS17535 0.12 -1.4 3,636,563 - BWI76_RS17535 0.12 +0.1 3,636,587 + BWI76_RS17535 0.13 -2.3 3,636,599 - BWI76_RS17535 0.13 +0.6 3,636,599 - BWI76_RS17535 0.13 +0.4 3,636,665 + BWI76_RS17535 0.15 -0.7 3,636,665 + BWI76_RS17535 0.15 -1.6 3,636,713 + BWI76_RS17535 0.17 -0.6 3,636,747 - BWI76_RS17535 0.18 +1.2 3,636,832 - BWI76_RS17535 0.21 +0.1 3,636,852 + BWI76_RS17535 0.21 +0.4 3,636,860 - BWI76_RS17535 0.22 +0.7
Or see this region's nucleotide sequence