Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17330 and BWI76_RS17335 overlap by 4 nucleotides BWI76_RS17335 and BWI76_RS17340 are separated by 396 nucleotides
BWI76_RS17330: BWI76_RS17330 - nitrate reductase subunit beta, at 3,586,776 to 3,588,311
_RS17330
BWI76_RS17335: BWI76_RS17335 - nitrate reductase subunit alpha, at 3,588,308 to 3,592,051
_RS17335
BWI76_RS17340: BWI76_RS17340 - NarK family nitrate/nitrite MFS transporter, at 3,592,448 to 3,593,836
_RS17340
Position (kb)
3588
3589
3590
3591
3592
3593 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3587.444 kb on - strand, within BWI76_RS17330 at 3587.501 kb on + strand, within BWI76_RS17330 at 3588.428 kb on + strand at 3588.483 kb on - strand at 3588.523 kb on + strand at 3588.872 kb on - strand, within BWI76_RS17335 at 3589.007 kb on - strand, within BWI76_RS17335 at 3589.276 kb on - strand, within BWI76_RS17335 at 3589.288 kb on + strand, within BWI76_RS17335 at 3590.009 kb on - strand, within BWI76_RS17335 at 3590.186 kb on - strand, within BWI76_RS17335 at 3590.388 kb on - strand, within BWI76_RS17335 at 3590.414 kb on + strand, within BWI76_RS17335 at 3591.207 kb on - strand, within BWI76_RS17335 at 3591.265 kb on + strand, within BWI76_RS17335 at 3591.317 kb on + strand, within BWI76_RS17335 at 3591.409 kb on + strand, within BWI76_RS17335 at 3591.522 kb on + strand, within BWI76_RS17335 at 3591.916 kb on + strand at 3591.958 kb on + strand at 3592.001 kb on + strand at 3592.009 kb on - strand at 3592.054 kb on + strand at 3592.054 kb on + strand at 3592.061 kb on + strand at 3592.064 kb on + strand at 3592.069 kb on - strand at 3592.071 kb on + strand at 3592.079 kb on - strand at 3592.080 kb on - strand at 3592.087 kb on - strand at 3592.116 kb on + strand at 3592.286 kb on + strand at 3592.311 kb on + strand at 3592.319 kb on - strand at 3592.344 kb on + strand at 3592.461 kb on + strand at 3592.471 kb on - strand at 3592.471 kb on - strand at 3592.502 kb on - strand at 3592.505 kb on - strand at 3592.541 kb on + strand at 3592.549 kb on - strand at 3592.549 kb on - strand at 3592.549 kb on - strand at 3592.634 kb on + strand, within BWI76_RS17340 at 3592.652 kb on + strand, within BWI76_RS17340 at 3592.711 kb on + strand, within BWI76_RS17340 at 3592.747 kb on + strand, within BWI76_RS17340
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 3,587,444 - BWI76_RS17330 0.43 +0.4 3,587,501 + BWI76_RS17330 0.47 -2.3 3,588,428 + +0.7 3,588,483 - +0.2 3,588,523 + +0.4 3,588,872 - BWI76_RS17335 0.15 +1.0 3,589,007 - BWI76_RS17335 0.19 +0.2 3,589,276 - BWI76_RS17335 0.26 +0.8 3,589,288 + BWI76_RS17335 0.26 +1.2 3,590,009 - BWI76_RS17335 0.45 -0.2 3,590,186 - BWI76_RS17335 0.50 +0.2 3,590,388 - BWI76_RS17335 0.56 +0.8 3,590,414 + BWI76_RS17335 0.56 +1.4 3,591,207 - BWI76_RS17335 0.77 -1.0 3,591,265 + BWI76_RS17335 0.79 +1.4 3,591,317 + BWI76_RS17335 0.80 +0.4 3,591,409 + BWI76_RS17335 0.83 +0.2 3,591,522 + BWI76_RS17335 0.86 +0.9 3,591,916 + +0.4 3,591,958 + +1.0 3,592,001 + -2.0 3,592,009 - +1.1 3,592,054 + +0.5 3,592,054 + -0.0 3,592,061 + +1.9 3,592,064 + +0.3 3,592,069 - +0.3 3,592,071 + +0.4 3,592,079 - -1.7 3,592,080 - +1.3 3,592,087 - +1.6 3,592,116 + +1.0 3,592,286 + -3.1 3,592,311 + +0.8 3,592,319 - +0.0 3,592,344 + +2.4 3,592,461 + -0.1 3,592,471 - +0.5 3,592,471 - -0.1 3,592,502 - +0.1 3,592,505 - -0.5 3,592,541 + +0.9 3,592,549 - +0.7 3,592,549 - +1.6 3,592,549 - +1.2 3,592,634 + BWI76_RS17340 0.13 +1.0 3,592,652 + BWI76_RS17340 0.15 +0.1 3,592,711 + BWI76_RS17340 0.19 -0.2 3,592,747 + BWI76_RS17340 0.22 +0.5
Or see this region's nucleotide sequence