Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17225 and BWI76_RS17230 are separated by 11 nucleotides BWI76_RS17230 and BWI76_RS17235 are separated by 14 nucleotides BWI76_RS17235 and BWI76_RS17240 are separated by 88 nucleotides
BWI76_RS17225: BWI76_RS17225 - oligopeptide ABC transporter ATP-binding protein OppD, at 3,567,714 to 3,568,727
_RS17225
BWI76_RS17230: BWI76_RS17230 - peptide ABC transporter permease, at 3,568,739 to 3,569,647
_RS17230
BWI76_RS17235: BWI76_RS17235 - peptide ABC transporter permease, at 3,569,662 to 3,570,582
_RS17235
BWI76_RS17240: BWI76_RS17240 - oligopeptide ABC transporter substrate-binding protein OppA, at 3,570,671 to 3,572,302
_RS17240
Position (kb)
3569
3570
3571 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3568.745 kb on - strand at 3568.882 kb on + strand, within BWI76_RS17230 at 3569.244 kb on - strand, within BWI76_RS17230 at 3569.287 kb on - strand, within BWI76_RS17230 at 3569.301 kb on + strand, within BWI76_RS17230 at 3569.759 kb on - strand, within BWI76_RS17235 at 3569.917 kb on + strand, within BWI76_RS17235 at 3569.970 kb on + strand, within BWI76_RS17235 at 3569.985 kb on - strand, within BWI76_RS17235 at 3570.185 kb on - strand, within BWI76_RS17235 at 3570.250 kb on + strand, within BWI76_RS17235 at 3570.312 kb on - strand, within BWI76_RS17235 at 3570.367 kb on - strand, within BWI76_RS17235 at 3570.462 kb on + strand, within BWI76_RS17235 at 3570.492 kb on + strand at 3570.492 kb on + strand at 3570.549 kb on + strand at 3570.549 kb on + strand at 3570.549 kb on + strand at 3570.584 kb on + strand at 3570.600 kb on - strand at 3570.600 kb on - strand at 3570.600 kb on - strand at 3570.600 kb on - strand at 3570.604 kb on + strand at 3570.645 kb on + strand at 3570.650 kb on + strand at 3570.658 kb on - strand at 3570.705 kb on + strand at 3570.713 kb on - strand at 3571.125 kb on + strand, within BWI76_RS17240 at 3571.133 kb on - strand, within BWI76_RS17240 at 3571.138 kb on + strand, within BWI76_RS17240 at 3571.146 kb on - strand, within BWI76_RS17240 at 3571.260 kb on + strand, within BWI76_RS17240 at 3571.343 kb on + strand, within BWI76_RS17240 at 3571.487 kb on + strand, within BWI76_RS17240
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 3,568,745 - -1.7 3,568,882 + BWI76_RS17230 0.16 +0.4 3,569,244 - BWI76_RS17230 0.56 +0.9 3,569,287 - BWI76_RS17230 0.60 -0.4 3,569,301 + BWI76_RS17230 0.62 -1.3 3,569,759 - BWI76_RS17235 0.11 +0.9 3,569,917 + BWI76_RS17235 0.28 -0.2 3,569,970 + BWI76_RS17235 0.33 +1.0 3,569,985 - BWI76_RS17235 0.35 +0.3 3,570,185 - BWI76_RS17235 0.57 +2.2 3,570,250 + BWI76_RS17235 0.64 +0.3 3,570,312 - BWI76_RS17235 0.71 +1.4 3,570,367 - BWI76_RS17235 0.77 +1.4 3,570,462 + BWI76_RS17235 0.87 +0.0 3,570,492 + +1.5 3,570,492 + +0.0 3,570,549 + +0.5 3,570,549 + +0.3 3,570,549 + +0.6 3,570,584 + -1.4 3,570,600 - -1.0 3,570,600 - -1.4 3,570,600 - -0.3 3,570,600 - +0.8 3,570,604 + -0.1 3,570,645 + +0.2 3,570,650 + +0.1 3,570,658 - -1.5 3,570,705 + +1.5 3,570,713 - -0.4 3,571,125 + BWI76_RS17240 0.28 +0.2 3,571,133 - BWI76_RS17240 0.28 -1.9 3,571,138 + BWI76_RS17240 0.29 -0.1 3,571,146 - BWI76_RS17240 0.29 -0.6 3,571,260 + BWI76_RS17240 0.36 +0.4 3,571,343 + BWI76_RS17240 0.41 +1.0 3,571,487 + BWI76_RS17240 0.50 -1.9
Or see this region's nucleotide sequence