Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16355 and BWI76_RS16360 overlap by 1 nucleotides BWI76_RS16360 and BWI76_RS16365 overlap by 8 nucleotides BWI76_RS16365 and BWI76_RS16370 are separated by 0 nucleotides
BWI76_RS16355: BWI76_RS16355 - electron transport complex subunit A, at 3,393,602 to 3,394,183
_RS16355
BWI76_RS16360: BWI76_RS16360 - electron transport complex subunit RsxB, at 3,394,183 to 3,394,761
_RS16360
BWI76_RS16365: BWI76_RS16365 - electron transport complex subunit RsxC, at 3,394,754 to 3,396,814
_RS16365
BWI76_RS16370: BWI76_RS16370 - electron transport complex subunit D, at 3,396,815 to 3,397,867
_RS16370
Position (kb)
3394
3395
3396
3397 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3393.970 kb on + strand, within BWI76_RS16355 at 3394.192 kb on + strand at 3394.369 kb on - strand, within BWI76_RS16360 at 3394.890 kb on + strand at 3395.046 kb on - strand, within BWI76_RS16365 at 3395.263 kb on - strand, within BWI76_RS16365 at 3395.451 kb on + strand, within BWI76_RS16365 at 3395.459 kb on - strand, within BWI76_RS16365 at 3395.459 kb on - strand at 3395.750 kb on - strand, within BWI76_RS16365 at 3396.157 kb on - strand, within BWI76_RS16365 at 3396.239 kb on + strand, within BWI76_RS16365 at 3396.242 kb on - strand, within BWI76_RS16365 at 3396.464 kb on + strand, within BWI76_RS16365 at 3396.730 kb on - strand at 3396.996 kb on + strand, within BWI76_RS16370 at 3396.996 kb on + strand, within BWI76_RS16370 at 3397.308 kb on + strand, within BWI76_RS16370 at 3397.364 kb on + strand, within BWI76_RS16370 at 3397.622 kb on + strand, within BWI76_RS16370
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 3,393,970 + BWI76_RS16355 0.63 +1.4 3,394,192 + +0.1 3,394,369 - BWI76_RS16360 0.32 -1.5 3,394,890 + -0.1 3,395,046 - BWI76_RS16365 0.14 -2.3 3,395,263 - BWI76_RS16365 0.25 -1.7 3,395,451 + BWI76_RS16365 0.34 -0.1 3,395,459 - BWI76_RS16365 0.34 +0.1 3,395,459 - -0.9 3,395,750 - BWI76_RS16365 0.48 -1.4 3,396,157 - BWI76_RS16365 0.68 -3.0 3,396,239 + BWI76_RS16365 0.72 +0.1 3,396,242 - BWI76_RS16365 0.72 -1.6 3,396,464 + BWI76_RS16365 0.83 -4.2 3,396,730 - +0.4 3,396,996 + BWI76_RS16370 0.17 +0.4 3,396,996 + BWI76_RS16370 0.17 -1.9 3,397,308 + BWI76_RS16370 0.47 -0.5 3,397,364 + BWI76_RS16370 0.52 -0.5 3,397,622 + BWI76_RS16370 0.77 -1.1
Or see this region's nucleotide sequence