Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15870 and BWI76_RS15875 are separated by 95 nucleotides BWI76_RS15875 and BWI76_RS15880 are separated by 26 nucleotides BWI76_RS15880 and BWI76_RS15885 are separated by 36 nucleotides BWI76_RS15885 and BWI76_RS15890 are separated by 177 nucleotides
BWI76_RS15870: BWI76_RS15870 - hypothetical protein, at 3,294,329 to 3,295,048
_RS15870
BWI76_RS15875: BWI76_RS15875 - hypothetical protein, at 3,295,144 to 3,295,383
_RS15875
BWI76_RS15880: BWI76_RS15880 - DNA-binding protein, at 3,295,410 to 3,295,679
_RS15880
BWI76_RS15885: BWI76_RS15885 - putative phage integrase, at 3,295,716 to 3,296,996
_RS15885
BWI76_RS15890: BWI76_RS15890 - Zn-dependent oxidoreductase, at 3,297,174 to 3,298,190
_RS15890
Position (kb) 3295
3296
3297 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 3295.027 kb on + strand at 3295.798 kb on - strand at 3295.912 kb on + strand, within BWI76_RS15885 at 3295.928 kb on + strand, within BWI76_RS15885 at 3295.949 kb on - strand, within BWI76_RS15885 at 3296.151 kb on + strand, within BWI76_RS15885 at 3296.153 kb on + strand, within BWI76_RS15885 at 3296.176 kb on - strand, within BWI76_RS15885 at 3296.305 kb on - strand, within BWI76_RS15885 at 3296.433 kb on - strand, within BWI76_RS15885 at 3296.434 kb on - strand, within BWI76_RS15885 at 3296.445 kb on - strand, within BWI76_RS15885 at 3296.596 kb on - strand, within BWI76_RS15885 at 3296.677 kb on - strand, within BWI76_RS15885 at 3296.682 kb on - strand, within BWI76_RS15885 at 3296.701 kb on + strand, within BWI76_RS15885 at 3296.708 kb on - strand, within BWI76_RS15885 at 3296.722 kb on + strand at 3296.814 kb on + strand, within BWI76_RS15885 at 3296.818 kb on + strand at 3296.934 kb on - strand at 3297.047 kb on + strand at 3297.047 kb on + strand at 3297.060 kb on - strand at 3297.065 kb on - strand at 3297.079 kb on - strand at 3297.145 kb on + strand at 3297.153 kb on - strand at 3297.157 kb on + strand at 3297.185 kb on - strand at 3297.202 kb on - strand at 3297.235 kb on - strand at 3297.238 kb on + strand at 3297.248 kb on + strand at 3297.268 kb on + strand at 3297.319 kb on + strand, within BWI76_RS15890 at 3297.371 kb on + strand, within BWI76_RS15890 at 3297.400 kb on - strand, within BWI76_RS15890 at 3297.597 kb on - strand, within BWI76_RS15890 at 3297.619 kb on + strand, within BWI76_RS15890 at 3297.637 kb on - strand at 3297.688 kb on + strand, within BWI76_RS15890 at 3297.696 kb on - strand, within BWI76_RS15890 at 3297.875 kb on + strand, within BWI76_RS15890 at 3297.880 kb on + strand, within BWI76_RS15890 at 3297.943 kb on + strand, within BWI76_RS15890
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 3,295,027 + +0.5 3,295,798 - -1.9 3,295,912 + BWI76_RS15885 0.15 +0.9 3,295,928 + BWI76_RS15885 0.17 -0.0 3,295,949 - BWI76_RS15885 0.18 +0.1 3,296,151 + BWI76_RS15885 0.34 -0.6 3,296,153 + BWI76_RS15885 0.34 -0.2 3,296,176 - BWI76_RS15885 0.36 -2.0 3,296,305 - BWI76_RS15885 0.46 -1.0 3,296,433 - BWI76_RS15885 0.56 +1.2 3,296,434 - BWI76_RS15885 0.56 -0.6 3,296,445 - BWI76_RS15885 0.57 -1.0 3,296,596 - BWI76_RS15885 0.69 +1.6 3,296,677 - BWI76_RS15885 0.75 +0.1 3,296,682 - BWI76_RS15885 0.75 -1.3 3,296,701 + BWI76_RS15885 0.77 -1.6 3,296,708 - BWI76_RS15885 0.77 -1.5 3,296,722 + +1.5 3,296,814 + BWI76_RS15885 0.86 +0.1 3,296,818 + +0.2 3,296,934 - -1.6 3,297,047 + +2.8 3,297,047 + +1.2 3,297,060 - -1.6 3,297,065 - +1.2 3,297,079 - +2.5 3,297,145 + +0.9 3,297,153 - -0.8 3,297,157 + -1.1 3,297,185 - -3.1 3,297,202 - -0.3 3,297,235 - +0.9 3,297,238 + -0.4 3,297,248 + +0.2 3,297,268 + -1.6 3,297,319 + BWI76_RS15890 0.14 +0.3 3,297,371 + BWI76_RS15890 0.19 +3.6 3,297,400 - BWI76_RS15890 0.22 -2.7 3,297,597 - BWI76_RS15890 0.42 -0.8 3,297,619 + BWI76_RS15890 0.44 -1.0 3,297,637 - -0.4 3,297,688 + BWI76_RS15890 0.51 +1.0 3,297,696 - BWI76_RS15890 0.51 +0.5 3,297,875 + BWI76_RS15890 0.69 +0.3 3,297,880 + BWI76_RS15890 0.69 +4.2 3,297,943 + BWI76_RS15890 0.76 +0.2
Or see this region's nucleotide sequence