Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14920 and BWI76_RS14925 overlap by 4 nucleotides BWI76_RS14925 and BWI76_RS14930 are separated by 24 nucleotides BWI76_RS14930 and BWI76_RS14935 are separated by 288 nucleotides BWI76_RS14935 and BWI76_RS14940 are separated by 139 nucleotides
BWI76_RS14920: BWI76_RS14920 - ABC transporter permease, at 3,127,403 to 3,128,410
_RS14920
BWI76_RS14925: BWI76_RS14925 - ABC transporter substrate-binding protein, at 3,128,407 to 3,129,363
_RS14925
BWI76_RS14930: BWI76_RS14930 - hypothetical protein, at 3,129,388 to 3,130,197
_RS14930
BWI76_RS14935: BWI76_RS14935 - NIPSNAP family containing protein, at 3,130,486 to 3,130,815
_RS14935
BWI76_RS14940: BWI76_RS14940 - hypothetical protein, at 3,130,955 to 3,131,803
_RS14940
Position (kb) 3129
3130
3131 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3128.672 kb on - strand, within BWI76_RS14925 at 3128.743 kb on + strand, within BWI76_RS14925 at 3128.910 kb on - strand, within BWI76_RS14925 at 3128.915 kb on - strand, within BWI76_RS14925 at 3129.255 kb on + strand, within BWI76_RS14925 at 3129.288 kb on - strand at 3129.397 kb on + strand at 3129.607 kb on + strand, within BWI76_RS14930 at 3130.446 kb on - strand at 3130.734 kb on - strand, within BWI76_RS14935 at 3130.933 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 3,128,672 - BWI76_RS14925 0.28 -0.8 3,128,743 + BWI76_RS14925 0.35 -2.4 3,128,910 - BWI76_RS14925 0.53 +0.2 3,128,915 - BWI76_RS14925 0.53 +0.5 3,129,255 + BWI76_RS14925 0.89 +1.8 3,129,288 - -2.0 3,129,397 + -0.0 3,129,607 + BWI76_RS14930 0.27 +1.4 3,130,446 - +0.5 3,130,734 - BWI76_RS14935 0.75 +0.1 3,130,933 + +0.9
Or see this region's nucleotide sequence