Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14540 and BWI76_RS14545 overlap by 4 nucleotides BWI76_RS14545 and BWI76_RS14550 are separated by 3 nucleotides BWI76_RS14550 and BWI76_RS14555 are separated by 129 nucleotides
BWI76_RS14540: BWI76_RS14540 - yersiniabactin biosynthetic protein YbtU, at 3,051,676 to 3,052,773
_RS14540
BWI76_RS14545: BWI76_RS14545 - thioesterase, at 3,052,770 to 3,053,573
_RS14545
BWI76_RS14550: BWI76_RS14550 - 2,3-dihydroxybenzoate-AMP ligase, at 3,053,577 to 3,055,154
_RS14550
BWI76_RS14555: BWI76_RS14555 - Yersiniabactin/pesticin outer membrane receptor (IRPC), at 3,055,284 to 3,057,305
_RS14555
Position (kb) 3053
3054
3055
3056 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3052.830 kb on + strand at 3052.914 kb on + strand, within BWI76_RS14545 at 3052.948 kb on + strand, within BWI76_RS14545 at 3053.344 kb on + strand, within BWI76_RS14545 at 3053.384 kb on - strand, within BWI76_RS14545 at 3053.562 kb on + strand at 3054.132 kb on - strand, within BWI76_RS14550 at 3054.161 kb on - strand, within BWI76_RS14550 at 3054.198 kb on + strand, within BWI76_RS14550 at 3054.216 kb on - strand, within BWI76_RS14550 at 3054.294 kb on - strand, within BWI76_RS14550 at 3054.327 kb on - strand, within BWI76_RS14550 at 3054.409 kb on - strand, within BWI76_RS14550 at 3054.475 kb on + strand, within BWI76_RS14550 at 3054.693 kb on + strand, within BWI76_RS14550 at 3054.723 kb on + strand, within BWI76_RS14550 at 3054.999 kb on - strand at 3055.463 kb on - strand at 3055.500 kb on + strand, within BWI76_RS14555 at 3055.605 kb on - strand, within BWI76_RS14555 at 3055.667 kb on - strand, within BWI76_RS14555 at 3056.098 kb on - strand, within BWI76_RS14555
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 3,052,830 + -1.6 3,052,914 + BWI76_RS14545 0.18 +0.1 3,052,948 + BWI76_RS14545 0.22 +0.2 3,053,344 + BWI76_RS14545 0.71 +0.1 3,053,384 - BWI76_RS14545 0.76 -0.3 3,053,562 + +0.3 3,054,132 - BWI76_RS14550 0.35 -1.1 3,054,161 - BWI76_RS14550 0.37 +1.1 3,054,198 + BWI76_RS14550 0.39 +0.1 3,054,216 - BWI76_RS14550 0.40 +0.9 3,054,294 - BWI76_RS14550 0.45 +0.6 3,054,327 - BWI76_RS14550 0.48 +0.0 3,054,409 - BWI76_RS14550 0.53 +0.6 3,054,475 + BWI76_RS14550 0.57 +0.8 3,054,693 + BWI76_RS14550 0.71 +1.8 3,054,723 + BWI76_RS14550 0.73 +0.2 3,054,999 - +0.2 3,055,463 - -0.2 3,055,500 + BWI76_RS14555 0.11 +1.2 3,055,605 - BWI76_RS14555 0.16 -1.9 3,055,667 - BWI76_RS14555 0.19 +1.4 3,056,098 - BWI76_RS14555 0.40 +1.4
Or see this region's nucleotide sequence