Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11745 and BWI76_RS11750 are separated by 12 nucleotides BWI76_RS11750 and BWI76_RS11755 are separated by 40 nucleotides BWI76_RS11755 and BWI76_RS11760 are separated by 97 nucleotides BWI76_RS11760 and BWI76_RS11765 are separated by 74 nucleotides
BWI76_RS11745: BWI76_RS11745 - 6-phospho-beta-glucosidase, at 2,470,400 to 2,471,746
_RS11745
BWI76_RS11750: BWI76_RS11750 - chitooligosaccharide deacetylase, at 2,471,759 to 2,472,517
_RS11750
BWI76_RS11755: BWI76_RS11755 - catalase HPII, at 2,472,558 to 2,474,816
_RS11755
BWI76_RS11760: BWI76_RS11760 - cell division protein, at 2,474,914 to 2,475,252
_RS11760
BWI76_RS11765: BWI76_RS11765 - bifunctional transporter/kinase, at 2,475,327 to 2,476,718
_RS11765
Position (kb)
2472
2473
2474
2475 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2471.580 kb on + strand, within BWI76_RS11745 at 2471.691 kb on + strand at 2471.713 kb on - strand at 2471.784 kb on - strand at 2471.861 kb on - strand, within BWI76_RS11750 at 2472.165 kb on - strand, within BWI76_RS11750 at 2472.333 kb on + strand, within BWI76_RS11750 at 2472.443 kb on - strand at 2472.520 kb on - strand at 2472.994 kb on + strand, within BWI76_RS11755 at 2473.066 kb on + strand, within BWI76_RS11755 at 2473.110 kb on - strand, within BWI76_RS11755 at 2473.303 kb on + strand, within BWI76_RS11755 at 2473.370 kb on + strand, within BWI76_RS11755 at 2473.437 kb on - strand, within BWI76_RS11755 at 2474.794 kb on + strand at 2475.122 kb on - strand, within BWI76_RS11760 at 2475.325 kb on + strand at 2475.325 kb on + strand at 2475.455 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 2,471,580 + BWI76_RS11745 0.88 +0.3 2,471,691 + +1.0 2,471,713 - -1.1 2,471,784 - +0.7 2,471,861 - BWI76_RS11750 0.13 +1.5 2,472,165 - BWI76_RS11750 0.53 +1.6 2,472,333 + BWI76_RS11750 0.76 -0.6 2,472,443 - +0.2 2,472,520 - +0.0 2,472,994 + BWI76_RS11755 0.19 +0.4 2,473,066 + BWI76_RS11755 0.22 -0.0 2,473,110 - BWI76_RS11755 0.24 +0.6 2,473,303 + BWI76_RS11755 0.33 +0.6 2,473,370 + BWI76_RS11755 0.36 +2.1 2,473,437 - BWI76_RS11755 0.39 +1.3 2,474,794 + +0.1 2,475,122 - BWI76_RS11760 0.61 +2.9 2,475,325 + +1.1 2,475,325 + +0.3 2,475,455 - +1.5
Or see this region's nucleotide sequence