Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11245

Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11240 and BWI76_RS11245 overlap by 4 nucleotidesBWI76_RS11245 and BWI76_RS11250 overlap by 4 nucleotidesBWI76_RS11250 and BWI76_RS11255 overlap by 17 nucleotides BWI76_RS11240: BWI76_RS11240 - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 2,371,207 to 2,372,253 _RS11240 BWI76_RS11245: BWI76_RS11245 - spermidine/putrescine ABC transporter permease PotC, at 2,372,250 to 2,373,035 _RS11245 BWI76_RS11250: BWI76_RS11250 - spermidine/putrescine ABC transporter permease PotB, at 2,373,032 to 2,373,889 _RS11250 BWI76_RS11255: BWI76_RS11255 - putrescine/spermidine ABC transporter ATP-binding protein, at 2,373,873 to 2,375,009 _RS11255 Position (kb) 2372 2373 2374Strain fitness (log2 ratio) -3 -2 -1 0 1at 2371.571 kb on + strand, within BWI76_RS11240at 2371.715 kb on + strand, within BWI76_RS11240at 2371.725 kb on + strand, within BWI76_RS11240at 2371.762 kb on - strand, within BWI76_RS11240at 2371.867 kb on - strand, within BWI76_RS11240at 2372.461 kb on - strand, within BWI76_RS11245at 2372.599 kb on - strand, within BWI76_RS11245at 2372.922 kb on + strand, within BWI76_RS11245at 2373.411 kb on + strand, within BWI76_RS11250at 2373.732 kb on - strand, within BWI76_RS11250at 2373.837 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
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2,371,571 + BWI76_RS11240 0.35 -0.7
2,371,715 + BWI76_RS11240 0.49 +0.4
2,371,725 + BWI76_RS11240 0.49 +0.2
2,371,762 - BWI76_RS11240 0.53 -3.7
2,371,867 - BWI76_RS11240 0.63 +0.3
2,372,461 - BWI76_RS11245 0.27 +0.9
2,372,599 - BWI76_RS11245 0.44 +0.8
2,372,922 + BWI76_RS11245 0.85 +0.3
2,373,411 + BWI76_RS11250 0.44 +0.1
2,373,732 - BWI76_RS11250 0.82 -0.6
2,373,837 - +0.1

Or see this region's nucleotide sequence