Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08805 and BWI76_RS08810 are separated by 28 nucleotides BWI76_RS08810 and BWI76_RS08815 are separated by 111 nucleotides BWI76_RS08815 and BWI76_RS08820 are separated by 11 nucleotides BWI76_RS08820 and BWI76_RS08825 are separated by 3 nucleotides
BWI76_RS08805: BWI76_RS08805 - gluconeogenesis factor, at 1,857,269 to 1,858,174
_RS08805
BWI76_RS08810: BWI76_RS08810 - hypothetical protein, at 1,858,203 to 1,858,397
_RS08810
BWI76_RS08815: BWI76_RS08815 - cyclic pyranopterin phosphate synthase, at 1,858,509 to 1,859,508
_RS08815
BWI76_RS08820: BWI76_RS08820 - molybdenum cofactor biosynthesis protein, at 1,859,520 to 1,860,032
_RS08820
BWI76_RS08825: BWI76_RS08825 - cyclic pyranopterin monophosphate synthase MoaC, at 1,860,036 to 1,860,524
_RS08825
Position (kb) 1858
1859
1860 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1857.550 kb on - strand, within BWI76_RS08805 at 1857.725 kb on + strand, within BWI76_RS08805 at 1857.978 kb on - strand, within BWI76_RS08805 at 1858.072 kb on + strand, within BWI76_RS08805 at 1858.214 kb on + strand at 1858.397 kb on + strand at 1858.397 kb on + strand at 1858.404 kb on - strand at 1858.505 kb on + strand at 1858.513 kb on + strand at 1858.833 kb on + strand, within BWI76_RS08815 at 1859.001 kb on - strand, within BWI76_RS08815 at 1859.011 kb on + strand, within BWI76_RS08815 at 1859.062 kb on + strand, within BWI76_RS08815 at 1859.252 kb on + strand, within BWI76_RS08815 at 1859.327 kb on + strand, within BWI76_RS08815 at 1859.536 kb on - strand at 1859.592 kb on + strand, within BWI76_RS08820 at 1859.821 kb on - strand, within BWI76_RS08820 at 1859.866 kb on - strand, within BWI76_RS08820 at 1859.965 kb on + strand, within BWI76_RS08820 at 1860.154 kb on - strand, within BWI76_RS08825 at 1860.268 kb on - strand, within BWI76_RS08825 at 1860.425 kb on - strand, within BWI76_RS08825
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 1,857,550 - BWI76_RS08805 0.31 -0.9 1,857,725 + BWI76_RS08805 0.50 -1.3 1,857,978 - BWI76_RS08805 0.78 +0.8 1,858,072 + BWI76_RS08805 0.89 +1.5 1,858,214 + -0.4 1,858,397 + +0.3 1,858,397 + +1.1 1,858,404 - +2.1 1,858,505 + +0.2 1,858,513 + +0.9 1,858,833 + BWI76_RS08815 0.32 +0.1 1,859,001 - BWI76_RS08815 0.49 +0.4 1,859,011 + BWI76_RS08815 0.50 +0.6 1,859,062 + BWI76_RS08815 0.55 +0.6 1,859,252 + BWI76_RS08815 0.74 +1.2 1,859,327 + BWI76_RS08815 0.82 +1.1 1,859,536 - +1.9 1,859,592 + BWI76_RS08820 0.14 -0.4 1,859,821 - BWI76_RS08820 0.59 +1.8 1,859,866 - BWI76_RS08820 0.67 +1.1 1,859,965 + BWI76_RS08820 0.87 -1.9 1,860,154 - BWI76_RS08825 0.24 +0.2 1,860,268 - BWI76_RS08825 0.47 +0.9 1,860,425 - BWI76_RS08825 0.80 +0.4
Or see this region's nucleotide sequence