Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04910 and BWI76_RS04915 are separated by 175 nucleotides BWI76_RS04915 and BWI76_RS04920 are separated by 31 nucleotides BWI76_RS04920 and BWI76_RS04925 are separated by 14 nucleotides
BWI76_RS04910: BWI76_RS04910 - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 1,029,429 to 1,032,047
_RS04910
BWI76_RS04915: BWI76_RS04915 - hypothetical protein, at 1,032,223 to 1,032,585
_RS04915
BWI76_RS04920: BWI76_RS04920 - S-adenosylmethionine decarboxylase, at 1,032,617 to 1,033,411
_RS04920
BWI76_RS04925: BWI76_RS04925 - spermidine synthase, at 1,033,426 to 1,034,289
_RS04925
Position (kb) 1032
1033
1034 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1032.184 kb on + strand at 1032.320 kb on + strand, within BWI76_RS04915 at 1032.326 kb on + strand, within BWI76_RS04915 at 1032.367 kb on + strand, within BWI76_RS04915 at 1032.367 kb on + strand, within BWI76_RS04915 at 1032.436 kb on + strand, within BWI76_RS04915 at 1032.587 kb on - strand at 1032.614 kb on + strand at 1032.614 kb on + strand at 1032.686 kb on - strand at 1032.736 kb on - strand, within BWI76_RS04920 at 1032.744 kb on - strand, within BWI76_RS04920 at 1032.809 kb on + strand, within BWI76_RS04920 at 1032.842 kb on + strand, within BWI76_RS04920 at 1032.927 kb on - strand, within BWI76_RS04920 at 1032.995 kb on + strand, within BWI76_RS04920 at 1033.156 kb on + strand, within BWI76_RS04920 at 1033.321 kb on + strand, within BWI76_RS04920 at 1033.428 kb on + strand at 1033.429 kb on - strand at 1033.450 kb on + strand at 1033.594 kb on + strand, within BWI76_RS04925 at 1033.602 kb on - strand, within BWI76_RS04925 at 1033.653 kb on + strand, within BWI76_RS04925 at 1033.717 kb on - strand, within BWI76_RS04925 at 1033.722 kb on - strand, within BWI76_RS04925 at 1033.874 kb on + strand, within BWI76_RS04925 at 1034.079 kb on + strand, within BWI76_RS04925 at 1034.087 kb on - strand, within BWI76_RS04925 at 1034.231 kb on + strand at 1034.278 kb on + strand at 1034.360 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 1,032,184 + -0.4 1,032,320 + BWI76_RS04915 0.27 -0.3 1,032,326 + BWI76_RS04915 0.28 +0.0 1,032,367 + BWI76_RS04915 0.40 +1.5 1,032,367 + BWI76_RS04915 0.40 +1.4 1,032,436 + BWI76_RS04915 0.59 -0.6 1,032,587 - -2.4 1,032,614 + -1.1 1,032,614 + +1.2 1,032,686 - +0.4 1,032,736 - BWI76_RS04920 0.15 +1.6 1,032,744 - BWI76_RS04920 0.16 -0.3 1,032,809 + BWI76_RS04920 0.24 +1.2 1,032,842 + BWI76_RS04920 0.28 +0.2 1,032,927 - BWI76_RS04920 0.39 +2.5 1,032,995 + BWI76_RS04920 0.48 -0.0 1,033,156 + BWI76_RS04920 0.68 -0.5 1,033,321 + BWI76_RS04920 0.89 -0.1 1,033,428 + -0.2 1,033,429 - -0.0 1,033,450 + -0.4 1,033,594 + BWI76_RS04925 0.19 -1.1 1,033,602 - BWI76_RS04925 0.20 -0.4 1,033,653 + BWI76_RS04925 0.26 -0.0 1,033,717 - BWI76_RS04925 0.34 +0.5 1,033,722 - BWI76_RS04925 0.34 -0.4 1,033,874 + BWI76_RS04925 0.52 -0.5 1,034,079 + BWI76_RS04925 0.76 -0.8 1,034,087 - BWI76_RS04925 0.77 +0.2 1,034,231 + -0.4 1,034,278 + +0.2 1,034,360 - +0.2
Or see this region's nucleotide sequence