Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04905 and BWI76_RS04910 are separated by 334 nucleotides BWI76_RS04910 and BWI76_RS04915 are separated by 175 nucleotides BWI76_RS04915 and BWI76_RS04920 are separated by 31 nucleotides
BWI76_RS04905: BWI76_RS04905 - DUF3300 domain-containing protein, at 1,027,508 to 1,029,094
_RS04905
BWI76_RS04910: BWI76_RS04910 - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 1,029,429 to 1,032,047
_RS04910
BWI76_RS04915: BWI76_RS04915 - hypothetical protein, at 1,032,223 to 1,032,585
_RS04915
BWI76_RS04920: BWI76_RS04920 - S-adenosylmethionine decarboxylase, at 1,032,617 to 1,033,411
_RS04920
Position (kb) 1029
1030
1031
1032
1033 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1028.712 kb on - strand, within BWI76_RS04905 at 1028.831 kb on - strand, within BWI76_RS04905 at 1028.846 kb on + strand, within BWI76_RS04905 at 1028.926 kb on - strand, within BWI76_RS04905 at 1028.926 kb on - strand, within BWI76_RS04905 at 1028.926 kb on - strand, within BWI76_RS04905 at 1028.962 kb on - strand at 1029.131 kb on + strand at 1029.241 kb on + strand at 1029.249 kb on - strand at 1029.276 kb on - strand at 1032.184 kb on + strand at 1032.320 kb on + strand, within BWI76_RS04915 at 1032.326 kb on + strand, within BWI76_RS04915 at 1032.367 kb on + strand, within BWI76_RS04915 at 1032.367 kb on + strand, within BWI76_RS04915 at 1032.436 kb on + strand, within BWI76_RS04915 at 1032.587 kb on - strand at 1032.614 kb on + strand at 1032.614 kb on + strand at 1032.686 kb on - strand at 1032.736 kb on - strand, within BWI76_RS04920 at 1032.744 kb on - strand, within BWI76_RS04920 at 1032.809 kb on + strand, within BWI76_RS04920 at 1032.842 kb on + strand, within BWI76_RS04920 at 1032.927 kb on - strand, within BWI76_RS04920 at 1032.995 kb on + strand, within BWI76_RS04920
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 1,028,712 - BWI76_RS04905 0.76 -2.0 1,028,831 - BWI76_RS04905 0.83 +0.7 1,028,846 + BWI76_RS04905 0.84 -0.4 1,028,926 - BWI76_RS04905 0.89 +0.5 1,028,926 - BWI76_RS04905 0.89 -0.1 1,028,926 - BWI76_RS04905 0.89 +0.1 1,028,962 - -1.1 1,029,131 + -0.8 1,029,241 + -0.9 1,029,249 - +0.1 1,029,276 - +1.1 1,032,184 + -0.4 1,032,320 + BWI76_RS04915 0.27 -0.3 1,032,326 + BWI76_RS04915 0.28 +0.0 1,032,367 + BWI76_RS04915 0.40 +1.5 1,032,367 + BWI76_RS04915 0.40 +1.4 1,032,436 + BWI76_RS04915 0.59 -0.6 1,032,587 - -2.4 1,032,614 + -1.1 1,032,614 + +1.2 1,032,686 - +0.4 1,032,736 - BWI76_RS04920 0.15 +1.6 1,032,744 - BWI76_RS04920 0.16 -0.3 1,032,809 + BWI76_RS04920 0.24 +1.2 1,032,842 + BWI76_RS04920 0.28 +0.2 1,032,927 - BWI76_RS04920 0.39 +2.5 1,032,995 + BWI76_RS04920 0.48 -0.0
Or see this region's nucleotide sequence