Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04465 and BWI76_RS04470 are separated by 18 nucleotides BWI76_RS04470 and BWI76_RS04475 are separated by 138 nucleotides
BWI76_RS04465: BWI76_RS04465 - carbamoyl-phosphate synthase small subunit, at 922,546 to 923,694
_RS04465
BWI76_RS04470: BWI76_RS04470 - carbamoyl phosphate synthase large subunit, at 923,713 to 926,937
_RS04470
BWI76_RS04475: BWI76_RS04475 - ROK family protein, at 927,076 to 927,960
_RS04475
Position (kb) 923
924
925
926
927 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 922.828 kb on + strand, within BWI76_RS04465 at 922.836 kb on + strand, within BWI76_RS04465 at 922.836 kb on - strand, within BWI76_RS04465 at 922.897 kb on - strand, within BWI76_RS04465 at 923.052 kb on - strand, within BWI76_RS04465 at 923.206 kb on + strand, within BWI76_RS04465 at 923.623 kb on - strand at 923.628 kb on - strand at 923.794 kb on - strand at 923.808 kb on + strand at 923.816 kb on - strand at 923.855 kb on + strand at 923.862 kb on + strand at 923.879 kb on - strand at 923.995 kb on + strand at 923.995 kb on + strand at 924.003 kb on - strand at 924.256 kb on + strand, within BWI76_RS04470 at 924.264 kb on - strand at 924.575 kb on - strand, within BWI76_RS04470 at 924.717 kb on + strand, within BWI76_RS04470 at 924.748 kb on - strand, within BWI76_RS04470 at 924.751 kb on - strand, within BWI76_RS04470 at 924.760 kb on - strand, within BWI76_RS04470 at 924.914 kb on + strand, within BWI76_RS04470 at 925.097 kb on + strand, within BWI76_RS04470 at 925.338 kb on + strand, within BWI76_RS04470 at 925.346 kb on - strand, within BWI76_RS04470 at 925.547 kb on + strand, within BWI76_RS04470 at 925.706 kb on + strand, within BWI76_RS04470 at 925.931 kb on - strand, within BWI76_RS04470 at 926.164 kb on - strand, within BWI76_RS04470 at 926.332 kb on - strand, within BWI76_RS04470 at 926.473 kb on + strand, within BWI76_RS04470 at 926.481 kb on - strand, within BWI76_RS04470 at 926.610 kb on + strand, within BWI76_RS04470 at 926.817 kb on + strand at 926.817 kb on + strand at 926.903 kb on - strand at 927.074 kb on - strand at 927.433 kb on + strand, within BWI76_RS04475 at 927.433 kb on + strand, within BWI76_RS04475 at 927.441 kb on - strand, within BWI76_RS04475 at 927.613 kb on - strand, within BWI76_RS04475 at 927.747 kb on + strand, within BWI76_RS04475 at 927.752 kb on + strand, within BWI76_RS04475 at 927.815 kb on + strand, within BWI76_RS04475 at 927.823 kb on - strand, within BWI76_RS04475 at 927.907 kb on + strand at 927.910 kb on - strand at 927.915 kb on - strand at 927.922 kb on + strand at 927.930 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 922,828 + BWI76_RS04465 0.25 -0.2 922,836 + BWI76_RS04465 0.25 -1.4 922,836 - BWI76_RS04465 0.25 -0.8 922,897 - BWI76_RS04465 0.31 -0.6 923,052 - BWI76_RS04465 0.44 -1.9 923,206 + BWI76_RS04465 0.57 -1.1 923,623 - +0.2 923,628 - -3.2 923,794 - -0.3 923,808 + -0.6 923,816 - +1.2 923,855 + +0.2 923,862 + -1.9 923,879 - -1.0 923,995 + +1.2 923,995 + -1.3 924,003 - -2.3 924,256 + BWI76_RS04470 0.17 -2.3 924,264 - -1.1 924,575 - BWI76_RS04470 0.27 +0.2 924,717 + BWI76_RS04470 0.31 +0.2 924,748 - BWI76_RS04470 0.32 +2.2 924,751 - BWI76_RS04470 0.32 -2.3 924,760 - BWI76_RS04470 0.32 -2.5 924,914 + BWI76_RS04470 0.37 -2.5 925,097 + BWI76_RS04470 0.43 -1.0 925,338 + BWI76_RS04470 0.50 +1.2 925,346 - BWI76_RS04470 0.51 -2.3 925,547 + BWI76_RS04470 0.57 +0.2 925,706 + BWI76_RS04470 0.62 -3.3 925,931 - BWI76_RS04470 0.69 -2.1 926,164 - BWI76_RS04470 0.76 -1.1 926,332 - BWI76_RS04470 0.81 -1.9 926,473 + BWI76_RS04470 0.86 +1.2 926,481 - BWI76_RS04470 0.86 -1.8 926,610 + BWI76_RS04470 0.90 -0.7 926,817 + -1.6 926,817 + +1.7 926,903 - -1.5 927,074 - -0.2 927,433 + BWI76_RS04475 0.40 +2.8 927,433 + BWI76_RS04475 0.40 -0.8 927,441 - BWI76_RS04475 0.41 -0.1 927,613 - BWI76_RS04475 0.61 +0.9 927,747 + BWI76_RS04475 0.76 +1.1 927,752 + BWI76_RS04475 0.76 -1.4 927,815 + BWI76_RS04475 0.84 +0.2 927,823 - BWI76_RS04475 0.84 +1.0 927,907 + -0.2 927,910 - +2.3 927,915 - -1.3 927,922 + +0.2 927,930 - +1.2
Or see this region's nucleotide sequence