Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02730 and BWI76_RS02735 are separated by 191 nucleotides BWI76_RS02735 and BWI76_RS02740 overlap by 11 nucleotides BWI76_RS02740 and BWI76_RS02745 are separated by 322 nucleotides
BWI76_RS02730: BWI76_RS02730 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase, at 552,455 to 554,398
_RS02730
BWI76_RS02735: BWI76_RS02735 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 554,590 to 555,333
_RS02735
BWI76_RS02740: BWI76_RS02740 - putative transcriptional regulator, at 555,323 to 555,880
_RS02740
BWI76_RS02745: BWI76_RS02745 - hypothetical protein, at 556,203 to 556,409
_RS02745
Position (kb) 554
555
556 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 553.902 kb on + strand, within BWI76_RS02730 at 553.991 kb on + strand, within BWI76_RS02730 at 554.159 kb on + strand, within BWI76_RS02730 at 554.203 kb on - strand, within BWI76_RS02730 at 554.227 kb on + strand at 554.227 kb on + strand at 554.267 kb on + strand at 554.380 kb on - strand at 554.390 kb on - strand at 554.431 kb on + strand at 554.567 kb on + strand at 554.567 kb on + strand at 554.736 kb on - strand, within BWI76_RS02735 at 554.803 kb on - strand, within BWI76_RS02735 at 554.963 kb on - strand, within BWI76_RS02735 at 555.079 kb on - strand, within BWI76_RS02735 at 555.173 kb on - strand, within BWI76_RS02735 at 555.207 kb on - strand, within BWI76_RS02735 at 555.478 kb on - strand, within BWI76_RS02740 at 555.568 kb on + strand, within BWI76_RS02740 at 555.634 kb on - strand, within BWI76_RS02740 at 555.683 kb on + strand, within BWI76_RS02740 at 555.683 kb on + strand, within BWI76_RS02740 at 555.763 kb on + strand, within BWI76_RS02740 at 555.775 kb on + strand, within BWI76_RS02740 at 555.798 kb on - strand, within BWI76_RS02740 at 555.874 kb on + strand at 555.882 kb on - strand at 555.883 kb on - strand at 556.228 kb on - strand, within BWI76_RS02745
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 553,902 + BWI76_RS02730 0.74 +0.8 553,991 + BWI76_RS02730 0.79 -2.4 554,159 + BWI76_RS02730 0.88 -0.8 554,203 - BWI76_RS02730 0.90 -0.6 554,227 + +0.2 554,227 + -0.3 554,267 + -0.7 554,380 - -1.7 554,390 - -0.1 554,431 + +1.7 554,567 + +0.9 554,567 + +0.3 554,736 - BWI76_RS02735 0.20 +0.5 554,803 - BWI76_RS02735 0.29 -3.7 554,963 - BWI76_RS02735 0.50 +1.5 555,079 - BWI76_RS02735 0.66 -1.6 555,173 - BWI76_RS02735 0.78 -0.9 555,207 - BWI76_RS02735 0.83 +0.5 555,478 - BWI76_RS02740 0.28 +2.1 555,568 + BWI76_RS02740 0.44 +0.1 555,634 - BWI76_RS02740 0.56 +0.1 555,683 + BWI76_RS02740 0.65 +1.4 555,683 + BWI76_RS02740 0.65 +0.6 555,763 + BWI76_RS02740 0.79 -2.7 555,775 + BWI76_RS02740 0.81 -0.8 555,798 - BWI76_RS02740 0.85 -0.5 555,874 + +0.4 555,882 - +0.5 555,883 - +0.0 556,228 - BWI76_RS02745 0.12 -1.2
Or see this region's nucleotide sequence