Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00510 and BWI76_RS00515 overlap by 4 nucleotides BWI76_RS00515 and BWI76_RS00520 are separated by 175 nucleotides
BWI76_RS00510: BWI76_RS00510 - glucuronyl hydrolase, at 103,932 to 105,110
_RS00510
BWI76_RS00515: BWI76_RS00515 - PTS fructose transporter subunit IIA, at 105,107 to 105,589
_RS00515
BWI76_RS00520: BWI76_RS00520 - transcription regulator of PTS system, at 105,765 to 108,368
_RS00520
Position (kb) 105
106 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 104.111 kb on + strand, within BWI76_RS00510 at 104.119 kb on - strand, within BWI76_RS00510 at 104.119 kb on - strand, within BWI76_RS00510 at 104.119 kb on - strand, within BWI76_RS00510 at 104.223 kb on - strand, within BWI76_RS00510 at 104.265 kb on + strand, within BWI76_RS00510 at 104.341 kb on + strand, within BWI76_RS00510 at 104.347 kb on - strand, within BWI76_RS00510 at 104.349 kb on - strand, within BWI76_RS00510 at 104.349 kb on - strand, within BWI76_RS00510 at 104.460 kb on - strand, within BWI76_RS00510 at 104.654 kb on - strand, within BWI76_RS00510 at 104.727 kb on - strand, within BWI76_RS00510 at 104.765 kb on - strand, within BWI76_RS00510 at 104.772 kb on - strand, within BWI76_RS00510 at 104.796 kb on - strand, within BWI76_RS00510 at 104.829 kb on + strand at 105.106 kb on + strand at 105.113 kb on - strand at 105.124 kb on - strand at 105.161 kb on - strand, within BWI76_RS00515 at 105.201 kb on + strand, within BWI76_RS00515 at 105.312 kb on + strand, within BWI76_RS00515 at 105.444 kb on - strand, within BWI76_RS00515 at 105.457 kb on - strand, within BWI76_RS00515 at 105.512 kb on - strand, within BWI76_RS00515 at 105.526 kb on + strand, within BWI76_RS00515 at 105.614 kb on - strand at 105.726 kb on - strand at 105.734 kb on - strand at 105.762 kb on + strand at 105.762 kb on + strand at 105.762 kb on + strand at 105.763 kb on + strand at 105.770 kb on - strand at 105.770 kb on - strand at 105.771 kb on - strand at 105.771 kb on - strand at 105.784 kb on - strand at 105.855 kb on + strand at 105.940 kb on - strand at 106.051 kb on + strand, within BWI76_RS00520 at 106.122 kb on - strand, within BWI76_RS00520 at 106.149 kb on - strand, within BWI76_RS00520 at 106.223 kb on + strand, within BWI76_RS00520 at 106.223 kb on + strand, within BWI76_RS00520 at 106.228 kb on + strand, within BWI76_RS00520 at 106.231 kb on - strand, within BWI76_RS00520 at 106.231 kb on - strand, within BWI76_RS00520 at 106.231 kb on - strand, within BWI76_RS00520 at 106.236 kb on - strand, within BWI76_RS00520 at 106.241 kb on + strand, within BWI76_RS00520 at 106.249 kb on - strand, within BWI76_RS00520 at 106.288 kb on - strand, within BWI76_RS00520 at 106.290 kb on + strand, within BWI76_RS00520 at 106.298 kb on - strand, within BWI76_RS00520 at 106.300 kb on + strand, within BWI76_RS00520 at 106.332 kb on + strand, within BWI76_RS00520 at 106.332 kb on + strand, within BWI76_RS00520 at 106.481 kb on + strand, within BWI76_RS00520 at 106.492 kb on + strand, within BWI76_RS00520 at 106.500 kb on - strand, within BWI76_RS00520 at 106.527 kb on - strand, within BWI76_RS00520 at 106.551 kb on + strand, within BWI76_RS00520 at 106.572 kb on - strand, within BWI76_RS00520
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 104,111 + BWI76_RS00510 0.15 +0.6 104,119 - BWI76_RS00510 0.16 -1.6 104,119 - BWI76_RS00510 0.16 +0.6 104,119 - BWI76_RS00510 0.16 +1.4 104,223 - BWI76_RS00510 0.25 +0.1 104,265 + BWI76_RS00510 0.28 +0.3 104,341 + BWI76_RS00510 0.35 +1.4 104,347 - BWI76_RS00510 0.35 -0.1 104,349 - BWI76_RS00510 0.35 -1.2 104,349 - BWI76_RS00510 0.35 -0.2 104,460 - BWI76_RS00510 0.45 +0.9 104,654 - BWI76_RS00510 0.61 -1.4 104,727 - BWI76_RS00510 0.67 -1.8 104,765 - BWI76_RS00510 0.71 +0.0 104,772 - BWI76_RS00510 0.71 -1.4 104,796 - BWI76_RS00510 0.73 -0.8 104,829 + +1.4 105,106 + -0.8 105,113 - +0.2 105,124 - +0.2 105,161 - BWI76_RS00515 0.11 +0.9 105,201 + BWI76_RS00515 0.19 +0.8 105,312 + BWI76_RS00515 0.42 -0.1 105,444 - BWI76_RS00515 0.70 +1.9 105,457 - BWI76_RS00515 0.72 +1.1 105,512 - BWI76_RS00515 0.84 -0.2 105,526 + BWI76_RS00515 0.87 -1.2 105,614 - +0.3 105,726 - -1.3 105,734 - -1.0 105,762 + -1.3 105,762 + +0.4 105,762 + -3.1 105,763 + -1.2 105,770 - -0.6 105,770 - -1.2 105,771 - -0.6 105,771 - +0.2 105,784 - -0.6 105,855 + +0.1 105,940 - +1.4 106,051 + BWI76_RS00520 0.11 -2.0 106,122 - BWI76_RS00520 0.14 +0.1 106,149 - BWI76_RS00520 0.15 +0.7 106,223 + BWI76_RS00520 0.18 +0.4 106,223 + BWI76_RS00520 0.18 +1.0 106,228 + BWI76_RS00520 0.18 +1.4 106,231 - BWI76_RS00520 0.18 +0.4 106,231 - BWI76_RS00520 0.18 -1.8 106,231 - BWI76_RS00520 0.18 +0.4 106,236 - BWI76_RS00520 0.18 -0.3 106,241 + BWI76_RS00520 0.18 -1.0 106,249 - BWI76_RS00520 0.19 +0.4 106,288 - BWI76_RS00520 0.20 -0.3 106,290 + BWI76_RS00520 0.20 +0.4 106,298 - BWI76_RS00520 0.20 -1.0 106,300 + BWI76_RS00520 0.21 +3.0 106,332 + BWI76_RS00520 0.22 +0.0 106,332 + BWI76_RS00520 0.22 -0.2 106,481 + BWI76_RS00520 0.27 -0.1 106,492 + BWI76_RS00520 0.28 +0.3 106,500 - BWI76_RS00520 0.28 -2.0 106,527 - BWI76_RS00520 0.29 -0.2 106,551 + BWI76_RS00520 0.30 +1.4 106,572 - BWI76_RS00520 0.31 -0.4
Or see this region's nucleotide sequence