Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00455 and BWI76_RS00460 overlap by 4 nucleotides BWI76_RS00460 and BWI76_RS00465 are separated by 12 nucleotides BWI76_RS00465 and BWI76_RS00470 are separated by 48 nucleotides BWI76_RS00470 and BWI76_RS00475 are separated by 156 nucleotides
BWI76_RS00455: BWI76_RS00455 - formate dehydrogenase cytochrome b556 subunit, at 93,214 to 93,849
_RS00455
BWI76_RS00460: BWI76_RS00460 - formate dehydrogenase subunit beta, at 93,846 to 94,748
_RS00460
BWI76_RS00465: BWI76_RS00465 - formate dehydrogenase-N subunit alpha, at 94,761 to 97,175
_RS00465
BWI76_RS00470: BWI76_RS00470 - sulfate ABC transporter substrate-binding protein, at 97,224 to 97,811
_RS00470
BWI76_RS00475: BWI76_RS00475 - sulfurtransferase FdhD, at 97,968 to 98,804
_RS00475
Position (kb) 94
95
96
97
98 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 94.744 kb on + strand at 94.789 kb on + strand at 94.789 kb on + strand at 95.082 kb on - strand, within BWI76_RS00465 at 95.176 kb on - strand, within BWI76_RS00465 at 95.176 kb on - strand, within BWI76_RS00465 at 95.501 kb on + strand, within BWI76_RS00465 at 95.501 kb on + strand, within BWI76_RS00465 at 95.503 kb on - strand, within BWI76_RS00465 at 95.509 kb on - strand, within BWI76_RS00465 at 95.518 kb on + strand, within BWI76_RS00465 at 96.205 kb on + strand, within BWI76_RS00465 at 96.456 kb on - strand, within BWI76_RS00465 at 96.574 kb on + strand, within BWI76_RS00465 at 96.574 kb on + strand, within BWI76_RS00465 at 96.582 kb on - strand, within BWI76_RS00465 at 96.582 kb on - strand, within BWI76_RS00465 at 96.582 kb on - strand, within BWI76_RS00465 at 96.604 kb on + strand, within BWI76_RS00465 at 96.813 kb on - strand, within BWI76_RS00465 at 96.861 kb on + strand, within BWI76_RS00465 at 97.211 kb on + strand at 97.222 kb on + strand at 97.349 kb on + strand, within BWI76_RS00470 at 97.406 kb on + strand, within BWI76_RS00470 at 97.492 kb on - strand, within BWI76_RS00470 at 97.529 kb on + strand, within BWI76_RS00470 at 97.529 kb on + strand, within BWI76_RS00470 at 97.529 kb on + strand, within BWI76_RS00470 at 97.529 kb on + strand, within BWI76_RS00470 at 97.534 kb on + strand, within BWI76_RS00470 at 97.537 kb on - strand, within BWI76_RS00470 at 97.637 kb on + strand, within BWI76_RS00470 at 97.660 kb on + strand, within BWI76_RS00470 at 97.668 kb on - strand, within BWI76_RS00470 at 97.668 kb on - strand, within BWI76_RS00470 at 97.701 kb on - strand, within BWI76_RS00470 at 97.737 kb on + strand, within BWI76_RS00470 at 97.774 kb on + strand at 97.793 kb on + strand at 97.806 kb on - strand at 97.882 kb on - strand at 97.944 kb on - strand at 98.110 kb on - strand, within BWI76_RS00475
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 94,744 + +0.5 94,789 + +1.4 94,789 + +0.2 95,082 - BWI76_RS00465 0.13 +1.2 95,176 - BWI76_RS00465 0.17 +0.2 95,176 - BWI76_RS00465 0.17 +0.7 95,501 + BWI76_RS00465 0.31 -0.2 95,501 + BWI76_RS00465 0.31 -0.6 95,503 - BWI76_RS00465 0.31 +1.4 95,509 - BWI76_RS00465 0.31 +0.0 95,518 + BWI76_RS00465 0.31 -0.2 96,205 + BWI76_RS00465 0.60 +0.8 96,456 - BWI76_RS00465 0.70 +0.7 96,574 + BWI76_RS00465 0.75 +0.6 96,574 + BWI76_RS00465 0.75 +0.5 96,582 - BWI76_RS00465 0.75 +0.3 96,582 - BWI76_RS00465 0.75 +0.5 96,582 - BWI76_RS00465 0.75 +1.6 96,604 + BWI76_RS00465 0.76 +0.6 96,813 - BWI76_RS00465 0.85 +2.2 96,861 + BWI76_RS00465 0.87 -0.8 97,211 + -0.1 97,222 + -0.5 97,349 + BWI76_RS00470 0.21 -1.6 97,406 + BWI76_RS00470 0.31 +0.9 97,492 - BWI76_RS00470 0.46 -1.6 97,529 + BWI76_RS00470 0.52 +1.7 97,529 + BWI76_RS00470 0.52 +0.7 97,529 + BWI76_RS00470 0.52 +0.6 97,529 + BWI76_RS00470 0.52 +1.6 97,534 + BWI76_RS00470 0.53 +1.4 97,537 - BWI76_RS00470 0.53 -0.0 97,637 + BWI76_RS00470 0.70 +1.0 97,660 + BWI76_RS00470 0.74 -2.3 97,668 - BWI76_RS00470 0.76 +1.1 97,668 - BWI76_RS00470 0.76 +1.1 97,701 - BWI76_RS00470 0.81 +1.0 97,737 + BWI76_RS00470 0.87 +1.0 97,774 + +0.4 97,793 + -0.2 97,806 - +0.4 97,882 - +0.6 97,944 - +3.9 98,110 - BWI76_RS00475 0.17 +1.6
Or see this region's nucleotide sequence