Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26040 and BWI76_RS26045 overlap by 4 nucleotides BWI76_RS26045 and BWI76_RS26050 are separated by 67 nucleotides BWI76_RS26050 and BWI76_RS26055 are separated by 11 nucleotides
BWI76_RS26040: BWI76_RS26040 - nitrite reductase large subunit, at 5,380,211 to 5,382,754
_RS26040
BWI76_RS26045: BWI76_RS26045 - nitrite reductase small subunit, at 5,382,751 to 5,383,077
_RS26045
BWI76_RS26050: BWI76_RS26050 - nitrite transporter NirC, at 5,383,145 to 5,383,954
_RS26050
BWI76_RS26055: BWI76_RS26055 - siroheme synthase, at 5,383,966 to 5,385,339
_RS26055
Position (kb) 5382
5383
5384 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5381.777 kb on - strand, within BWI76_RS26040 at 5382.201 kb on - strand, within BWI76_RS26040 at 5382.236 kb on - strand, within BWI76_RS26040 at 5382.285 kb on - strand, within BWI76_RS26040 at 5382.342 kb on + strand, within BWI76_RS26040 at 5382.533 kb on - strand at 5382.859 kb on + strand, within BWI76_RS26045 at 5383.007 kb on - strand, within BWI76_RS26045 at 5383.017 kb on - strand, within BWI76_RS26045 at 5383.124 kb on + strand at 5383.182 kb on - strand at 5383.182 kb on - strand at 5383.254 kb on + strand, within BWI76_RS26050 at 5383.262 kb on - strand, within BWI76_RS26050 at 5383.346 kb on + strand, within BWI76_RS26050 at 5383.354 kb on - strand, within BWI76_RS26050 at 5383.436 kb on - strand, within BWI76_RS26050 at 5383.447 kb on - strand, within BWI76_RS26050 at 5383.629 kb on - strand, within BWI76_RS26050 at 5383.703 kb on + strand, within BWI76_RS26050 at 5383.765 kb on + strand, within BWI76_RS26050 at 5383.773 kb on - strand, within BWI76_RS26050 at 5383.773 kb on - strand, within BWI76_RS26050 at 5383.791 kb on + strand, within BWI76_RS26050 at 5383.791 kb on + strand, within BWI76_RS26050 at 5383.798 kb on - strand, within BWI76_RS26050 at 5383.827 kb on + strand, within BWI76_RS26050 at 5383.837 kb on + strand, within BWI76_RS26050 at 5384.040 kb on + strand at 5384.048 kb on - strand at 5384.048 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 5,381,777 - BWI76_RS26040 0.62 -0.1 5,382,201 - BWI76_RS26040 0.78 +1.2 5,382,236 - BWI76_RS26040 0.80 -0.1 5,382,285 - BWI76_RS26040 0.82 +0.5 5,382,342 + BWI76_RS26040 0.84 +0.1 5,382,533 - +0.3 5,382,859 + BWI76_RS26045 0.33 -1.1 5,383,007 - BWI76_RS26045 0.78 -1.3 5,383,017 - BWI76_RS26045 0.81 +0.1 5,383,124 + -1.9 5,383,182 - -0.2 5,383,182 - -1.7 5,383,254 + BWI76_RS26050 0.13 +0.6 5,383,262 - BWI76_RS26050 0.14 +0.2 5,383,346 + BWI76_RS26050 0.25 +0.4 5,383,354 - BWI76_RS26050 0.26 -0.8 5,383,436 - BWI76_RS26050 0.36 -0.6 5,383,447 - BWI76_RS26050 0.37 +1.1 5,383,629 - BWI76_RS26050 0.60 +3.1 5,383,703 + BWI76_RS26050 0.69 -0.3 5,383,765 + BWI76_RS26050 0.77 +0.4 5,383,773 - BWI76_RS26050 0.78 -0.4 5,383,773 - BWI76_RS26050 0.78 -0.5 5,383,791 + BWI76_RS26050 0.80 +0.0 5,383,791 + BWI76_RS26050 0.80 -0.7 5,383,798 - BWI76_RS26050 0.81 +2.1 5,383,827 + BWI76_RS26050 0.84 +0.7 5,383,837 + BWI76_RS26050 0.85 -1.2 5,384,040 + -0.3 5,384,048 - -3.1 5,384,048 - +0.5
Or see this region's nucleotide sequence