Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24595 and BWI76_RS24600 are separated by 10 nucleotides BWI76_RS24600 and BWI76_RS24605 are separated by 10 nucleotides BWI76_RS24605 and BWI76_RS24610 are separated by 66 nucleotides
BWI76_RS24595: BWI76_RS24595 - dihydroxyacetone kinase subunit DhaK, at 5,126,406 to 5,127,476
_RS24595
BWI76_RS24600: BWI76_RS24600 - dihydroxyacetone kinase, at 5,127,487 to 5,128,119
_RS24600
BWI76_RS24605: BWI76_RS24605 - dihydroxyacetone kinase subunit DhaM, at 5,128,130 to 5,129,551
_RS24605
BWI76_RS24610: BWI76_RS24610 - dihydroxyacetone kinase, at 5,129,618 to 5,131,267
_RS24610
Position (kb) 5128
5129
5130 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5127.184 kb on - strand, within BWI76_RS24595 at 5127.384 kb on - strand at 5127.401 kb on + strand at 5127.444 kb on + strand at 5127.444 kb on + strand at 5127.452 kb on - strand at 5127.456 kb on + strand at 5127.456 kb on + strand at 5127.464 kb on - strand at 5127.464 kb on - strand at 5127.526 kb on - strand at 5127.624 kb on + strand, within BWI76_RS24600 at 5127.940 kb on - strand, within BWI76_RS24600 at 5128.013 kb on - strand, within BWI76_RS24600 at 5128.087 kb on + strand at 5128.266 kb on + strand at 5128.357 kb on - strand, within BWI76_RS24605 at 5128.375 kb on + strand, within BWI76_RS24605 at 5128.419 kb on - strand, within BWI76_RS24605 at 5128.437 kb on - strand, within BWI76_RS24605 at 5128.650 kb on + strand, within BWI76_RS24605 at 5128.676 kb on + strand, within BWI76_RS24605 at 5128.773 kb on + strand, within BWI76_RS24605 at 5128.773 kb on + strand, within BWI76_RS24605 at 5128.781 kb on - strand, within BWI76_RS24605 at 5128.918 kb on + strand, within BWI76_RS24605 at 5128.931 kb on - strand, within BWI76_RS24605 at 5129.094 kb on - strand, within BWI76_RS24605 at 5129.143 kb on - strand, within BWI76_RS24605 at 5129.226 kb on + strand, within BWI76_RS24605 at 5129.280 kb on + strand, within BWI76_RS24605 at 5129.295 kb on + strand, within BWI76_RS24605 at 5129.311 kb on + strand, within BWI76_RS24605 at 5129.321 kb on + strand, within BWI76_RS24605 at 5129.391 kb on - strand, within BWI76_RS24605 at 5129.391 kb on - strand, within BWI76_RS24605 at 5129.391 kb on - strand, within BWI76_RS24605 at 5129.405 kb on - strand, within BWI76_RS24605 at 5129.507 kb on + strand at 5129.577 kb on - strand at 5129.710 kb on + strand at 5130.313 kb on - strand, within BWI76_RS24610 at 5130.313 kb on - strand, within BWI76_RS24610 at 5130.313 kb on - strand, within BWI76_RS24610 at 5130.313 kb on - strand, within BWI76_RS24610 at 5130.346 kb on + strand, within BWI76_RS24610
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 5,127,184 - BWI76_RS24595 0.73 -0.3 5,127,384 - -0.5 5,127,401 + -1.1 5,127,444 + -1.3 5,127,444 + +0.1 5,127,452 - +0.0 5,127,456 + +0.2 5,127,456 + -0.5 5,127,464 - -1.6 5,127,464 - +1.0 5,127,526 - +0.0 5,127,624 + BWI76_RS24600 0.22 -2.5 5,127,940 - BWI76_RS24600 0.72 -0.5 5,128,013 - BWI76_RS24600 0.83 -0.6 5,128,087 + -2.7 5,128,266 + +0.0 5,128,357 - BWI76_RS24605 0.16 -1.0 5,128,375 + BWI76_RS24605 0.17 -1.5 5,128,419 - BWI76_RS24605 0.20 -1.0 5,128,437 - BWI76_RS24605 0.22 -1.2 5,128,650 + BWI76_RS24605 0.37 -0.5 5,128,676 + BWI76_RS24605 0.38 +1.0 5,128,773 + BWI76_RS24605 0.45 +1.0 5,128,773 + BWI76_RS24605 0.45 -2.5 5,128,781 - BWI76_RS24605 0.46 -0.5 5,128,918 + BWI76_RS24605 0.55 -1.3 5,128,931 - BWI76_RS24605 0.56 -2.8 5,129,094 - BWI76_RS24605 0.68 -1.0 5,129,143 - BWI76_RS24605 0.71 -1.3 5,129,226 + BWI76_RS24605 0.77 -1.0 5,129,280 + BWI76_RS24605 0.81 -2.0 5,129,295 + BWI76_RS24605 0.82 +1.0 5,129,311 + BWI76_RS24605 0.83 +0.9 5,129,321 + BWI76_RS24605 0.84 -1.4 5,129,391 - BWI76_RS24605 0.89 -3.7 5,129,391 - BWI76_RS24605 0.89 -1.5 5,129,391 - BWI76_RS24605 0.89 -0.5 5,129,405 - BWI76_RS24605 0.90 +1.0 5,129,507 + -2.4 5,129,577 - -1.5 5,129,710 + +0.0 5,130,313 - BWI76_RS24610 0.42 -2.8 5,130,313 - BWI76_RS24610 0.42 +1.0 5,130,313 - BWI76_RS24610 0.42 -0.0 5,130,313 - BWI76_RS24610 0.42 +1.4 5,130,346 + BWI76_RS24610 0.44 +1.3
Or see this region's nucleotide sequence