Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22635 and BWI76_RS22640 overlap by 4 nucleotides BWI76_RS22640 and BWI76_RS22645 are separated by 65 nucleotides BWI76_RS22645 and BWI76_RS22650 are separated by 158 nucleotides
BWI76_RS22635: BWI76_RS22635 - MFS transporter, at 4,718,449 to 4,719,663
_RS22635
BWI76_RS22640: BWI76_RS22640 - hypothetical protein, at 4,719,660 to 4,720,037
_RS22640
BWI76_RS22645: BWI76_RS22645 - RNA polymerase sigma factor RpoS, at 4,720,103 to 4,721,095
_RS22645
BWI76_RS22650: BWI76_RS22650 - lipoprotein NlpD, at 4,721,254 to 4,722,372
_RS22650
Position (kb) 4720
4721
4722 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4719.391 kb on + strand, within BWI76_RS22635 at 4719.399 kb on - strand, within BWI76_RS22635 at 4719.458 kb on - strand, within BWI76_RS22635 at 4719.536 kb on + strand, within BWI76_RS22635 at 4719.652 kb on - strand at 4719.904 kb on + strand, within BWI76_RS22640 at 4719.917 kb on - strand, within BWI76_RS22640 at 4720.041 kb on - strand at 4720.112 kb on + strand at 4720.220 kb on + strand, within BWI76_RS22645 at 4720.220 kb on + strand, within BWI76_RS22645 at 4720.258 kb on + strand, within BWI76_RS22645 at 4720.279 kb on - strand, within BWI76_RS22645 at 4720.785 kb on - strand, within BWI76_RS22645 at 4720.919 kb on - strand, within BWI76_RS22645 at 4721.005 kb on - strand at 4721.125 kb on - strand at 4721.329 kb on + strand at 4721.337 kb on - strand at 4721.337 kb on - strand at 4721.344 kb on + strand at 4721.347 kb on - strand at 4721.475 kb on + strand, within BWI76_RS22650 at 4721.526 kb on + strand, within BWI76_RS22650 at 4721.746 kb on + strand, within BWI76_RS22650 at 4721.746 kb on + strand, within BWI76_RS22650 at 4722.010 kb on - strand, within BWI76_RS22650 at 4722.040 kb on - strand, within BWI76_RS22650 at 4722.093 kb on - strand, within BWI76_RS22650
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 4,719,391 + BWI76_RS22635 0.78 +0.5 4,719,399 - BWI76_RS22635 0.78 -1.0 4,719,458 - BWI76_RS22635 0.83 +1.8 4,719,536 + BWI76_RS22635 0.89 -0.1 4,719,652 - -0.7 4,719,904 + BWI76_RS22640 0.65 +0.3 4,719,917 - BWI76_RS22640 0.68 -0.1 4,720,041 - -1.4 4,720,112 + -1.9 4,720,220 + BWI76_RS22645 0.12 -1.5 4,720,220 + BWI76_RS22645 0.12 -1.2 4,720,258 + BWI76_RS22645 0.16 -1.1 4,720,279 - BWI76_RS22645 0.18 -2.1 4,720,785 - BWI76_RS22645 0.69 -0.8 4,720,919 - BWI76_RS22645 0.82 -1.3 4,721,005 - -2.3 4,721,125 - -1.2 4,721,329 + -0.1 4,721,337 - -2.4 4,721,337 - -0.8 4,721,344 + -1.2 4,721,347 - -1.3 4,721,475 + BWI76_RS22650 0.20 -2.6 4,721,526 + BWI76_RS22650 0.24 -0.7 4,721,746 + BWI76_RS22650 0.44 +0.9 4,721,746 + BWI76_RS22650 0.44 -1.3 4,722,010 - BWI76_RS22650 0.68 +0.6 4,722,040 - BWI76_RS22650 0.70 -0.7 4,722,093 - BWI76_RS22650 0.75 -1.2
Or see this region's nucleotide sequence