Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22425 and BWI76_RS22430 overlap by 4 nucleotides BWI76_RS22430 and BWI76_RS22435 overlap by 4 nucleotides BWI76_RS22435 and BWI76_RS22440 overlap by 7 nucleotides
BWI76_RS22425: BWI76_RS22425 - metal ABC transporter substrate-binding protein, at 4,676,159 to 4,677,076
_RS22425
BWI76_RS22430: BWI76_RS22430 - manganese/iron transporter ATP-binding protein, at 4,677,073 to 4,677,894
_RS22430
BWI76_RS22435: BWI76_RS22435 - iron ABC transporter permease AfeC, at 4,677,891 to 4,678,739
_RS22435
BWI76_RS22440: BWI76_RS22440 - iron ABC transporter permease, at 4,678,733 to 4,679,587
_RS22440
Position (kb) 4677
4678 Strain fitness (log2 ratio) -2
-1
0
1 at 4676.162 kb on - strand at 4676.162 kb on - strand at 4676.183 kb on + strand at 4676.191 kb on - strand at 4676.220 kb on - strand at 4676.463 kb on - strand, within BWI76_RS22425 at 4676.521 kb on + strand, within BWI76_RS22425 at 4676.646 kb on - strand, within BWI76_RS22425 at 4676.724 kb on - strand, within BWI76_RS22425 at 4676.764 kb on - strand, within BWI76_RS22425 at 4676.816 kb on - strand, within BWI76_RS22425 at 4677.107 kb on + strand at 4677.363 kb on + strand, within BWI76_RS22430 at 4677.648 kb on + strand, within BWI76_RS22430 at 4677.811 kb on + strand, within BWI76_RS22430 at 4677.819 kb on - strand at 4678.036 kb on + strand, within BWI76_RS22435 at 4678.079 kb on - strand, within BWI76_RS22435 at 4678.174 kb on + strand, within BWI76_RS22435 at 4678.296 kb on - strand, within BWI76_RS22435 at 4678.351 kb on + strand, within BWI76_RS22435 at 4678.516 kb on - strand, within BWI76_RS22435 at 4678.561 kb on - strand, within BWI76_RS22435 at 4678.747 kb on + strand at 4678.747 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 4,676,162 - +1.0 4,676,162 - -0.1 4,676,183 + -0.9 4,676,191 - -0.0 4,676,220 - -0.3 4,676,463 - BWI76_RS22425 0.33 +0.9 4,676,521 + BWI76_RS22425 0.39 +0.7 4,676,646 - BWI76_RS22425 0.53 -1.3 4,676,724 - BWI76_RS22425 0.62 +1.0 4,676,764 - BWI76_RS22425 0.66 +0.2 4,676,816 - BWI76_RS22425 0.72 +1.0 4,677,107 + -1.1 4,677,363 + BWI76_RS22430 0.35 -2.5 4,677,648 + BWI76_RS22430 0.70 -1.0 4,677,811 + BWI76_RS22430 0.90 -1.1 4,677,819 - +0.4 4,678,036 + BWI76_RS22435 0.17 +1.0 4,678,079 - BWI76_RS22435 0.22 +0.9 4,678,174 + BWI76_RS22435 0.33 +1.4 4,678,296 - BWI76_RS22435 0.48 -0.2 4,678,351 + BWI76_RS22435 0.54 +0.3 4,678,516 - BWI76_RS22435 0.74 +0.5 4,678,561 - BWI76_RS22435 0.79 +0.0 4,678,747 + -0.2 4,678,747 + -0.1
Or see this region's nucleotide sequence