Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20055 and BWI76_RS20060 overlap by 4 nucleotides BWI76_RS20060 and BWI76_RS20065 overlap by 1 nucleotides BWI76_RS20065 and BWI76_RS20070 are separated by 14 nucleotides
BWI76_RS20055: BWI76_RS20055 - 2-succinylbenzoate-CoA ligase, at 4,201,216 to 4,202,607
_RS20055
BWI76_RS20060: BWI76_RS20060 - o-succinylbenzoate synthase, at 4,202,604 to 4,203,569
_RS20060
BWI76_RS20065: BWI76_RS20065 - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 4,203,569 to 4,204,426
_RS20065
BWI76_RS20070: BWI76_RS20070 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 4,204,441 to 4,205,199
_RS20070
Position (kb) 4202
4203
4204 Strain fitness (log2 ratio) -1
0
1
2 at 4201.754 kb on - strand, within BWI76_RS20055 at 4202.020 kb on - strand, within BWI76_RS20055 at 4202.519 kb on - strand at 4202.549 kb on + strand at 4202.602 kb on + strand at 4202.610 kb on - strand at 4202.632 kb on - strand at 4202.687 kb on + strand at 4202.728 kb on - strand, within BWI76_RS20060 at 4202.798 kb on + strand, within BWI76_RS20060 at 4202.935 kb on - strand, within BWI76_RS20060 at 4203.063 kb on - strand, within BWI76_RS20060 at 4203.151 kb on + strand, within BWI76_RS20060 at 4203.174 kb on - strand, within BWI76_RS20060 at 4203.306 kb on - strand, within BWI76_RS20060 at 4203.763 kb on - strand, within BWI76_RS20065 at 4203.827 kb on - strand, within BWI76_RS20065 at 4203.913 kb on + strand, within BWI76_RS20065 at 4204.105 kb on + strand, within BWI76_RS20065 at 4204.489 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 4,201,754 - BWI76_RS20055 0.39 +0.4 4,202,020 - BWI76_RS20055 0.58 -0.5 4,202,519 - +2.5 4,202,549 + -0.4 4,202,602 + +0.0 4,202,610 - +0.4 4,202,632 - -0.4 4,202,687 + -0.7 4,202,728 - BWI76_RS20060 0.13 -1.4 4,202,798 + BWI76_RS20060 0.20 -0.1 4,202,935 - BWI76_RS20060 0.34 -0.7 4,203,063 - BWI76_RS20060 0.48 -0.9 4,203,151 + BWI76_RS20060 0.57 -1.1 4,203,174 - BWI76_RS20060 0.59 +0.6 4,203,306 - BWI76_RS20060 0.73 +0.0 4,203,763 - BWI76_RS20065 0.23 -0.4 4,203,827 - BWI76_RS20065 0.30 -0.5 4,203,913 + BWI76_RS20065 0.40 -0.0 4,204,105 + BWI76_RS20065 0.62 -0.6 4,204,489 + -0.3
Or see this region's nucleotide sequence