Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19905 and BWI76_RS19910 are separated by 771 nucleotides BWI76_RS19910 and BWI76_RS19915 are separated by 16 nucleotides BWI76_RS19915 and BWI76_RS19920 are separated by 43 nucleotides
BWI76_RS19905: BWI76_RS19905 - OmpK36 porin, at 4,163,055 to 4,164,173
_RS19905
BWI76_RS19910: BWI76_RS19910 - phosphotransferase RcsD, at 4,164,945 to 4,167,605
_RS19910
BWI76_RS19915: BWI76_RS19915 - DNA-binding response regulator, at 4,167,622 to 4,168,272
_RS19915
BWI76_RS19920: BWI76_RS19920 - hybrid sensor histidine kinase/response regulator, at 4,168,316 to 4,171,162
_RS19920
Position (kb)
4164
4165
4166
4167
4168 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4163.968 kb on + strand, within BWI76_RS19905 at 4164.012 kb on - strand, within BWI76_RS19905 at 4164.029 kb on - strand, within BWI76_RS19905 at 4164.029 kb on - strand, within BWI76_RS19905 at 4164.029 kb on - strand, within BWI76_RS19905 at 4164.074 kb on + strand at 4164.074 kb on + strand at 4164.074 kb on + strand at 4164.074 kb on + strand at 4164.082 kb on - strand at 4164.082 kb on - strand at 4164.082 kb on - strand at 4164.112 kb on - strand at 4164.135 kb on - strand at 4164.161 kb on - strand at 4164.218 kb on + strand at 4164.218 kb on + strand at 4164.240 kb on - strand at 4164.433 kb on + strand at 4164.457 kb on + strand at 4164.536 kb on + strand at 4164.541 kb on + strand at 4164.549 kb on - strand at 4164.549 kb on - strand at 4164.603 kb on + strand at 4164.678 kb on + strand at 4164.681 kb on - strand at 4164.725 kb on - strand at 4164.762 kb on + strand at 4164.762 kb on - strand at 4164.769 kb on + strand at 4164.770 kb on - strand at 4164.787 kb on - strand at 4164.787 kb on - strand at 4164.825 kb on - strand at 4164.827 kb on + strand at 4164.833 kb on - strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.881 kb on - strand at 4164.881 kb on - strand at 4164.881 kb on - strand at 4164.883 kb on + strand at 4164.928 kb on - strand at 4164.930 kb on - strand at 4164.930 kb on - strand at 4165.189 kb on + strand at 4165.435 kb on + strand, within BWI76_RS19910 at 4165.512 kb on - strand, within BWI76_RS19910 at 4165.562 kb on + strand, within BWI76_RS19910 at 4165.572 kb on - strand, within BWI76_RS19910 at 4165.805 kb on + strand, within BWI76_RS19910 at 4165.813 kb on - strand, within BWI76_RS19910 at 4165.876 kb on + strand, within BWI76_RS19910 at 4165.901 kb on + strand, within BWI76_RS19910 at 4166.070 kb on - strand, within BWI76_RS19910 at 4166.173 kb on + strand, within BWI76_RS19910 at 4166.173 kb on + strand, within BWI76_RS19910 at 4166.251 kb on + strand, within BWI76_RS19910 at 4166.449 kb on - strand, within BWI76_RS19910 at 4166.629 kb on - strand, within BWI76_RS19910 at 4166.653 kb on + strand, within BWI76_RS19910 at 4166.971 kb on + strand, within BWI76_RS19910 at 4167.194 kb on + strand, within BWI76_RS19910 at 4167.311 kb on + strand, within BWI76_RS19910 at 4167.377 kb on - strand at 4167.412 kb on - strand at 4167.574 kb on + strand at 4167.595 kb on - strand at 4167.654 kb on + strand at 4168.101 kb on - strand, within BWI76_RS19915 at 4168.214 kb on + strand at 4168.313 kb on + strand at 4168.504 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 4,163,968 + BWI76_RS19905 0.82 -2.8 4,164,012 - BWI76_RS19905 0.86 -3.7 4,164,029 - BWI76_RS19905 0.87 -2.2 4,164,029 - BWI76_RS19905 0.87 -2.1 4,164,029 - BWI76_RS19905 0.87 -1.1 4,164,074 + +3.9 4,164,074 + -1.6 4,164,074 + -0.6 4,164,074 + -0.1 4,164,082 - +1.1 4,164,082 - -1.8 4,164,082 - -2.9 4,164,112 - -0.1 4,164,135 - -0.1 4,164,161 - -0.7 4,164,218 + -0.7 4,164,218 + -2.5 4,164,240 - -3.1 4,164,433 + +0.4 4,164,457 + +0.8 4,164,536 + +1.2 4,164,541 + +1.2 4,164,549 - +0.3 4,164,549 - +1.4 4,164,603 + +0.2 4,164,678 + -1.1 4,164,681 - +1.1 4,164,725 - +1.4 4,164,762 + +0.3 4,164,762 - +0.4 4,164,769 + +0.6 4,164,770 - -0.7 4,164,787 - -1.5 4,164,787 - -0.5 4,164,825 - -0.6 4,164,827 + +0.4 4,164,833 - -1.3 4,164,855 + +0.3 4,164,855 + -0.1 4,164,855 + -0.3 4,164,855 + +0.1 4,164,855 + +0.5 4,164,855 + +0.6 4,164,855 + +0.5 4,164,855 + -0.2 4,164,855 + -0.3 4,164,863 - -0.9 4,164,863 - -1.1 4,164,863 - -1.3 4,164,863 - +0.9 4,164,863 - -0.9 4,164,863 - -1.3 4,164,863 - -1.7 4,164,863 - -1.1 4,164,881 - -0.7 4,164,881 - -0.8 4,164,881 - -2.1 4,164,883 + -0.5 4,164,928 - -1.3 4,164,930 - -3.9 4,164,930 - -2.8 4,165,189 + -1.5 4,165,435 + BWI76_RS19910 0.18 -1.1 4,165,512 - BWI76_RS19910 0.21 -2.8 4,165,562 + BWI76_RS19910 0.23 -1.7 4,165,572 - BWI76_RS19910 0.24 -0.4 4,165,805 + BWI76_RS19910 0.32 -1.6 4,165,813 - BWI76_RS19910 0.33 -1.2 4,165,876 + BWI76_RS19910 0.35 -3.2 4,165,901 + BWI76_RS19910 0.36 -2.0 4,166,070 - BWI76_RS19910 0.42 -0.4 4,166,173 + BWI76_RS19910 0.46 -1.0 4,166,173 + BWI76_RS19910 0.46 -0.9 4,166,251 + BWI76_RS19910 0.49 -1.6 4,166,449 - BWI76_RS19910 0.57 -1.9 4,166,629 - BWI76_RS19910 0.63 -1.1 4,166,653 + BWI76_RS19910 0.64 -1.7 4,166,971 + BWI76_RS19910 0.76 -1.1 4,167,194 + BWI76_RS19910 0.85 +0.3 4,167,311 + BWI76_RS19910 0.89 -1.4 4,167,377 - -1.6 4,167,412 - -4.0 4,167,574 + -2.4 4,167,595 - -1.8 4,167,654 + -1.1 4,168,101 - BWI76_RS19915 0.74 -2.8 4,168,214 + -2.3 4,168,313 + +1.0 4,168,504 - -0.9
Or see this region's nucleotide sequence