Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18645 and BWI76_RS18650 are separated by 71 nucleotides BWI76_RS18650 and BWI76_RS18655 are separated by 46 nucleotides BWI76_RS18655 and BWI76_RS18660 are separated by 75 nucleotides
BWI76_RS18645: BWI76_RS18645 - type-1 fimbrial protein subunit A, at 3,865,545 to 3,866,090
_RS18645
BWI76_RS18650: BWI76_RS18650 - fimbrial protein, at 3,866,162 to 3,866,698
_RS18650
BWI76_RS18655: BWI76_RS18655 - chaperone FimC, periplasmic, at 3,866,745 to 3,867,455
_RS18655
BWI76_RS18660: BWI76_RS18660 - hypothetical protein, at 3,867,531 to 3,870,158
_RS18660
Position (kb) 3866
3867
3868 Strain fitness (log2 ratio) -2
-1
0
1 at 3865.756 kb on + strand, within BWI76_RS18645 at 3865.759 kb on - strand, within BWI76_RS18645 at 3865.761 kb on + strand, within BWI76_RS18645 at 3865.887 kb on - strand, within BWI76_RS18645 at 3865.907 kb on - strand, within BWI76_RS18645 at 3865.909 kb on + strand, within BWI76_RS18645 at 3865.990 kb on + strand, within BWI76_RS18645 at 3866.119 kb on + strand at 3866.394 kb on + strand, within BWI76_RS18650 at 3866.404 kb on + strand, within BWI76_RS18650 at 3866.438 kb on + strand, within BWI76_RS18650 at 3866.517 kb on + strand, within BWI76_RS18650 at 3866.525 kb on - strand, within BWI76_RS18650 at 3866.552 kb on - strand, within BWI76_RS18650 at 3866.849 kb on + strand, within BWI76_RS18655 at 3867.285 kb on - strand, within BWI76_RS18655 at 3867.421 kb on + strand at 3867.473 kb on + strand at 3867.474 kb on + strand at 3867.512 kb on + strand at 3867.580 kb on - strand at 3867.653 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 3,865,756 + BWI76_RS18645 0.39 +1.4 3,865,759 - BWI76_RS18645 0.39 -0.3 3,865,761 + BWI76_RS18645 0.40 -0.4 3,865,887 - BWI76_RS18645 0.63 +1.6 3,865,907 - BWI76_RS18645 0.66 -2.0 3,865,909 + BWI76_RS18645 0.67 -2.3 3,865,990 + BWI76_RS18645 0.82 +0.2 3,866,119 + +0.5 3,866,394 + BWI76_RS18650 0.43 +0.5 3,866,404 + BWI76_RS18650 0.45 +0.5 3,866,438 + BWI76_RS18650 0.51 +0.1 3,866,517 + BWI76_RS18650 0.66 -1.5 3,866,525 - BWI76_RS18650 0.68 -1.5 3,866,552 - BWI76_RS18650 0.73 -0.9 3,866,849 + BWI76_RS18655 0.15 +0.9 3,867,285 - BWI76_RS18655 0.76 +0.7 3,867,421 + -0.4 3,867,473 + +0.5 3,867,474 + +0.1 3,867,512 + +0.2 3,867,580 - +0.6 3,867,653 + -1.4
Or see this region's nucleotide sequence