Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18055 and BWI76_RS18060 are separated by 4 nucleotides BWI76_RS18060 and BWI76_RS18065 are separated by 135 nucleotides BWI76_RS18065 and BWI76_RS18070 are separated by 78 nucleotides BWI76_RS18070 and BWI76_RS18075 are separated by 6 nucleotides
BWI76_RS18055: BWI76_RS18055 - copper resistance protein D, at 3,752,076 to 3,752,945
_RS18055
BWI76_RS18060: BWI76_RS18060 - hypothetical protein, at 3,752,950 to 3,753,324
_RS18060
BWI76_RS18065: BWI76_RS18065 - DNA polymerase III subunit theta, at 3,753,460 to 3,753,690
_RS18065
BWI76_RS18070: BWI76_RS18070 - DNA polymerase III subunit epsilon, at 3,753,769 to 3,754,428
_RS18070
BWI76_RS18075: BWI76_RS18075 - oligopeptidase B, at 3,754,435 to 3,756,495
_RS18075
Position (kb) 3753
3754
3755 Strain fitness (log2 ratio) -1
0
1 at 3752.929 kb on - strand at 3753.135 kb on - strand, within BWI76_RS18060 at 3753.172 kb on + strand, within BWI76_RS18060 at 3753.197 kb on - strand, within BWI76_RS18060 at 3753.367 kb on - strand at 3753.402 kb on + strand at 3753.536 kb on + strand, within BWI76_RS18065 at 3753.590 kb on + strand at 3754.286 kb on + strand, within BWI76_RS18070 at 3754.287 kb on - strand, within BWI76_RS18070 at 3754.293 kb on - strand, within BWI76_RS18070 at 3754.385 kb on + strand at 3754.385 kb on + strand at 3754.447 kb on - strand at 3754.661 kb on - strand, within BWI76_RS18075 at 3754.942 kb on - strand, within BWI76_RS18075 at 3755.332 kb on - strand, within BWI76_RS18075 at 3755.332 kb on - strand, within BWI76_RS18075
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 3,752,929 - +0.4 3,753,135 - BWI76_RS18060 0.49 -0.3 3,753,172 + BWI76_RS18060 0.59 +1.1 3,753,197 - BWI76_RS18060 0.66 -0.1 3,753,367 - +0.8 3,753,402 + +0.1 3,753,536 + BWI76_RS18065 0.33 +0.7 3,753,590 + +0.8 3,754,286 + BWI76_RS18070 0.78 -0.5 3,754,287 - BWI76_RS18070 0.78 +0.2 3,754,293 - BWI76_RS18070 0.79 -1.1 3,754,385 + -0.3 3,754,385 + -1.2 3,754,447 - +0.9 3,754,661 - BWI76_RS18075 0.11 +0.2 3,754,942 - BWI76_RS18075 0.25 +0.5 3,755,332 - BWI76_RS18075 0.44 +0.0 3,755,332 - BWI76_RS18075 0.44 +0.4
Or see this region's nucleotide sequence