Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15240 and BWI76_RS15245 are separated by 18 nucleotides BWI76_RS15245 and BWI76_RS15250 are separated by 111 nucleotides BWI76_RS15250 and BWI76_RS15255 are separated by 28 nucleotides
BWI76_RS15240: BWI76_RS15240 - alcohol dehydrogenase, at 3,194,208 to 3,195,353
_RS15240
BWI76_RS15245: BWI76_RS15245 - dihydrodipicolinate synthase family protein, at 3,195,372 to 3,196,256
_RS15245
BWI76_RS15250: BWI76_RS15250 - tellurium resistance protein TerC, at 3,196,368 to 3,197,129
_RS15250
BWI76_RS15255: BWI76_RS15255 - hypothetical protein, at 3,197,158 to 3,198,330
_RS15255
Position (kb)
3195
3196
3197 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3194.389 kb on - strand, within BWI76_RS15240 at 3194.419 kb on - strand, within BWI76_RS15240 at 3194.522 kb on - strand, within BWI76_RS15240 at 3194.658 kb on - strand, within BWI76_RS15240 at 3195.052 kb on + strand, within BWI76_RS15240 at 3195.225 kb on + strand, within BWI76_RS15240 at 3195.356 kb on - strand at 3195.623 kb on - strand, within BWI76_RS15245 at 3195.702 kb on - strand, within BWI76_RS15245 at 3195.736 kb on - strand, within BWI76_RS15245 at 3195.892 kb on - strand, within BWI76_RS15245 at 3195.930 kb on - strand, within BWI76_RS15245 at 3195.962 kb on + strand, within BWI76_RS15245 at 3196.052 kb on + strand, within BWI76_RS15245 at 3196.074 kb on - strand, within BWI76_RS15245 at 3196.077 kb on + strand, within BWI76_RS15245 at 3196.077 kb on + strand, within BWI76_RS15245 at 3196.077 kb on + strand, within BWI76_RS15245 at 3196.077 kb on + strand, within BWI76_RS15245 at 3196.077 kb on + strand, within BWI76_RS15245 at 3196.085 kb on - strand, within BWI76_RS15245 at 3196.088 kb on - strand, within BWI76_RS15245 at 3196.098 kb on - strand, within BWI76_RS15245 at 3196.173 kb on - strand at 3196.173 kb on - strand at 3196.186 kb on + strand at 3196.191 kb on + strand at 3196.194 kb on - strand at 3196.197 kb on + strand at 3196.198 kb on + strand at 3196.199 kb on - strand at 3196.204 kb on + strand at 3196.205 kb on - strand at 3196.205 kb on - strand at 3196.205 kb on - strand at 3196.212 kb on - strand at 3196.212 kb on - strand at 3196.212 kb on - strand at 3196.351 kb on + strand at 3196.533 kb on + strand, within BWI76_RS15250 at 3197.100 kb on + strand at 3197.199 kb on + strand at 3197.205 kb on + strand at 3197.213 kb on - strand at 3197.224 kb on + strand at 3197.232 kb on + strand at 3197.232 kb on + strand at 3197.237 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 3,194,389 - BWI76_RS15240 0.16 +0.5 3,194,419 - BWI76_RS15240 0.18 +0.1 3,194,522 - BWI76_RS15240 0.27 -3.7 3,194,658 - BWI76_RS15240 0.39 -0.1 3,195,052 + BWI76_RS15240 0.74 +0.3 3,195,225 + BWI76_RS15240 0.89 -1.1 3,195,356 - +0.2 3,195,623 - BWI76_RS15245 0.28 +0.5 3,195,702 - BWI76_RS15245 0.37 +0.3 3,195,736 - BWI76_RS15245 0.41 +0.4 3,195,892 - BWI76_RS15245 0.59 +0.7 3,195,930 - BWI76_RS15245 0.63 -2.1 3,195,962 + BWI76_RS15245 0.67 -0.1 3,196,052 + BWI76_RS15245 0.77 +1.2 3,196,074 - BWI76_RS15245 0.79 +0.5 3,196,077 + BWI76_RS15245 0.80 +2.0 3,196,077 + BWI76_RS15245 0.80 +0.2 3,196,077 + BWI76_RS15245 0.80 -0.2 3,196,077 + BWI76_RS15245 0.80 +2.1 3,196,077 + BWI76_RS15245 0.80 +1.1 3,196,085 - BWI76_RS15245 0.81 -1.1 3,196,088 - BWI76_RS15245 0.81 +0.2 3,196,098 - BWI76_RS15245 0.82 +0.8 3,196,173 - -1.5 3,196,173 - +0.1 3,196,186 + +0.9 3,196,191 + +1.1 3,196,194 - +0.2 3,196,197 + +0.7 3,196,198 + -1.0 3,196,199 - +0.3 3,196,204 + -0.0 3,196,205 - +0.4 3,196,205 - -1.9 3,196,205 - +0.1 3,196,212 - -1.2 3,196,212 - -1.3 3,196,212 - -0.7 3,196,351 + -1.1 3,196,533 + BWI76_RS15250 0.22 +0.1 3,197,100 + +0.4 3,197,199 + +2.5 3,197,205 + +0.0 3,197,213 - +0.9 3,197,224 + -0.3 3,197,232 + -0.4 3,197,232 + +0.5 3,197,237 + -0.1
Or see this region's nucleotide sequence