Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13815 and BWI76_RS13820 are separated by 322 nucleotides BWI76_RS13820 and BWI76_RS13825 overlap by 8 nucleotides BWI76_RS13825 and BWI76_RS13830 are separated by 152 nucleotides
BWI76_RS13815: BWI76_RS13815 - ABC transporter, at 2,881,554 to 2,883,266
_RS13815
BWI76_RS13820: BWI76_RS13820 - hypothetical protein, at 2,883,589 to 2,884,269
_RS13820
BWI76_RS13825: BWI76_RS13825 - hypothetical protein, at 2,884,262 to 2,884,957
_RS13825
BWI76_RS13830: BWI76_RS13830 - NAD-dependent malic enzyme, at 2,885,110 to 2,886,807
_RS13830
Position (kb)
2883
2884
2885 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2882.654 kb on + strand, within BWI76_RS13815 at 2883.045 kb on - strand, within BWI76_RS13815 at 2883.045 kb on - strand, within BWI76_RS13815 at 2883.080 kb on + strand, within BWI76_RS13815 at 2883.088 kb on - strand, within BWI76_RS13815 at 2883.250 kb on + strand at 2883.405 kb on + strand at 2883.405 kb on + strand at 2883.413 kb on - strand at 2883.413 kb on - strand at 2883.413 kb on - strand at 2883.502 kb on + strand at 2883.513 kb on - strand at 2883.521 kb on - strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.533 kb on + strand at 2883.534 kb on + strand at 2883.534 kb on + strand at 2883.534 kb on + strand at 2883.541 kb on - strand at 2883.542 kb on - strand at 2883.548 kb on + strand at 2883.556 kb on - strand at 2883.556 kb on - strand at 2883.561 kb on - strand at 2883.566 kb on + strand at 2883.566 kb on + strand at 2883.569 kb on - strand at 2883.571 kb on + strand at 2883.571 kb on + strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.574 kb on - strand at 2883.579 kb on - strand at 2883.579 kb on - strand at 2883.579 kb on - strand at 2883.579 kb on - strand at 2883.584 kb on - strand at 2883.651 kb on + strand at 2883.652 kb on - strand at 2883.652 kb on - strand at 2883.652 kb on - strand at 2883.652 kb on - strand at 2883.676 kb on - strand, within BWI76_RS13820 at 2883.676 kb on - strand, within BWI76_RS13820 at 2883.676 kb on - strand, within BWI76_RS13820 at 2883.676 kb on - strand, within BWI76_RS13820 at 2884.102 kb on - strand, within BWI76_RS13820 at 2884.352 kb on - strand, within BWI76_RS13825 at 2884.985 kb on + strand at 2885.034 kb on + strand at 2885.034 kb on + strand at 2885.034 kb on + strand at 2885.068 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.070 kb on + strand at 2885.078 kb on - strand at 2885.078 kb on - strand at 2885.078 kb on - strand at 2885.078 kb on - strand at 2885.142 kb on - strand at 2885.155 kb on - strand at 2885.176 kb on - strand at 2885.222 kb on + strand at 2885.222 kb on + strand at 2885.224 kb on + strand at 2885.230 kb on - strand at 2885.230 kb on - strand at 2885.230 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 2,882,654 + BWI76_RS13815 0.64 +1.7 2,883,045 - BWI76_RS13815 0.87 +0.8 2,883,045 - BWI76_RS13815 0.87 +1.4 2,883,080 + BWI76_RS13815 0.89 -0.0 2,883,088 - BWI76_RS13815 0.90 -1.2 2,883,250 + -0.9 2,883,405 + -0.5 2,883,405 + -0.5 2,883,413 - -2.4 2,883,413 - +0.1 2,883,413 - +0.8 2,883,502 + -0.6 2,883,513 - -3.4 2,883,521 - -1.2 2,883,533 + +0.2 2,883,533 + -0.6 2,883,533 + +0.7 2,883,533 + -0.5 2,883,533 + -0.1 2,883,533 + +0.2 2,883,533 + +0.6 2,883,533 + -0.2 2,883,534 + -0.9 2,883,534 + -0.0 2,883,534 + +0.2 2,883,541 - +0.8 2,883,542 - -1.2 2,883,548 + +0.3 2,883,556 - -0.8 2,883,556 - +1.6 2,883,561 - -2.6 2,883,566 + +0.4 2,883,566 + -0.2 2,883,569 - +0.8 2,883,571 + -0.7 2,883,571 + -0.2 2,883,574 - -0.4 2,883,574 - -1.2 2,883,574 - -0.9 2,883,574 - -0.4 2,883,574 - -0.3 2,883,574 - -2.2 2,883,574 - -1.6 2,883,574 - -2.8 2,883,579 - -0.2 2,883,579 - +0.3 2,883,579 - +0.4 2,883,579 - -2.2 2,883,584 - -0.2 2,883,651 + +0.9 2,883,652 - +0.2 2,883,652 - -1.9 2,883,652 - -0.8 2,883,652 - -0.1 2,883,676 - BWI76_RS13820 0.13 +0.8 2,883,676 - BWI76_RS13820 0.13 -0.8 2,883,676 - BWI76_RS13820 0.13 -2.6 2,883,676 - BWI76_RS13820 0.13 -1.2 2,884,102 - BWI76_RS13820 0.75 -0.8 2,884,352 - BWI76_RS13825 0.13 +0.8 2,884,985 + -2.6 2,885,034 + +0.3 2,885,034 + +0.8 2,885,034 + +0.8 2,885,068 + +1.1 2,885,070 + +0.5 2,885,070 + -1.1 2,885,070 + -0.1 2,885,070 + -0.4 2,885,070 + +0.4 2,885,070 + +0.8 2,885,070 + -2.3 2,885,070 + -0.5 2,885,070 + -0.8 2,885,070 + -0.2 2,885,070 + -0.5 2,885,070 + +0.9 2,885,070 + +0.5 2,885,070 + +0.8 2,885,070 + -0.1 2,885,070 + +1.3 2,885,070 + -0.4 2,885,070 + -0.8 2,885,070 + -1.1 2,885,070 + -1.0 2,885,070 + +0.5 2,885,070 + -0.2 2,885,070 + +0.2 2,885,070 + -1.2 2,885,070 + -0.7 2,885,070 + +1.2 2,885,070 + +0.3 2,885,070 + +0.4 2,885,070 + +0.3 2,885,070 + -0.0 2,885,070 + +0.2 2,885,070 + -2.8 2,885,070 + -1.7 2,885,070 + +0.5 2,885,070 + +0.1 2,885,078 - -1.1 2,885,078 - -0.5 2,885,078 - +0.2 2,885,078 - +1.8 2,885,142 - -0.1 2,885,155 - -1.1 2,885,176 - -0.8 2,885,222 + -0.6 2,885,222 + -0.5 2,885,224 + +1.9 2,885,230 - -1.6 2,885,230 - +0.7 2,885,230 - +1.1
Or see this region's nucleotide sequence