Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13745 and BWI76_RS13750 overlap by 8 nucleotides BWI76_RS13750 and BWI76_RS13755 are separated by 41 nucleotides BWI76_RS13755 and BWI76_RS13760 overlap by 1 nucleotides BWI76_RS13760 and BWI76_RS13765 are separated by 356 nucleotides
BWI76_RS13745: BWI76_RS13745 - formate dehydrogenase subunit beta, at 2,867,397 to 2,868,281
_RS13745
BWI76_RS13750: BWI76_RS13750 - formate dehydrogenase, at 2,868,274 to 2,868,930
_RS13750
BWI76_RS13755: BWI76_RS13755 - hypothetical protein, at 2,868,972 to 2,869,295
_RS13755
BWI76_RS13760: BWI76_RS13760 - hypothetical protein, at 2,869,295 to 2,869,483
_RS13760
BWI76_RS13765: BWI76_RS13765 - MFS transporter AraJ, at 2,869,840 to 2,870,991
_RS13765
Position (kb)
2868
2869
2870 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2868.041 kb on + strand, within BWI76_RS13745 at 2868.076 kb on + strand, within BWI76_RS13745 at 2868.094 kb on + strand, within BWI76_RS13745 at 2868.114 kb on - strand, within BWI76_RS13745 at 2868.358 kb on - strand, within BWI76_RS13750 at 2868.403 kb on + strand, within BWI76_RS13750 at 2868.411 kb on - strand, within BWI76_RS13750 at 2868.556 kb on - strand, within BWI76_RS13750 at 2868.573 kb on + strand, within BWI76_RS13750 at 2868.573 kb on + strand, within BWI76_RS13750 at 2868.573 kb on + strand, within BWI76_RS13750 at 2868.654 kb on + strand, within BWI76_RS13750 at 2868.711 kb on + strand, within BWI76_RS13750 at 2868.719 kb on + strand, within BWI76_RS13750 at 2868.719 kb on + strand, within BWI76_RS13750 at 2868.824 kb on - strand, within BWI76_RS13750 at 2868.827 kb on + strand, within BWI76_RS13750 at 2868.827 kb on + strand, within BWI76_RS13750 at 2868.830 kb on - strand, within BWI76_RS13750 at 2868.865 kb on - strand at 2868.883 kb on + strand at 2868.886 kb on - strand at 2868.931 kb on + strand at 2868.960 kb on + strand at 2868.968 kb on - strand at 2868.970 kb on + strand at 2868.978 kb on - strand at 2869.189 kb on - strand, within BWI76_RS13755 at 2869.191 kb on + strand, within BWI76_RS13755 at 2869.264 kb on + strand at 2869.265 kb on + strand at 2869.293 kb on + strand at 2869.303 kb on + strand at 2869.310 kb on + strand at 2869.310 kb on - strand at 2869.387 kb on - strand, within BWI76_RS13760 at 2869.392 kb on - strand at 2869.393 kb on + strand, within BWI76_RS13760 at 2869.435 kb on - strand at 2869.460 kb on + strand, within BWI76_RS13760 at 2869.468 kb on - strand at 2869.503 kb on - strand at 2869.549 kb on + strand at 2869.549 kb on + strand at 2869.549 kb on + strand at 2869.554 kb on + strand at 2869.557 kb on - strand at 2869.562 kb on - strand at 2869.581 kb on - strand at 2869.656 kb on + strand at 2869.661 kb on + strand at 2869.663 kb on + strand at 2869.664 kb on - strand at 2869.666 kb on + strand at 2869.669 kb on - strand at 2869.669 kb on - strand at 2869.679 kb on - strand at 2869.679 kb on - strand at 2869.993 kb on + strand, within BWI76_RS13765 at 2870.009 kb on + strand, within BWI76_RS13765 at 2870.014 kb on + strand, within BWI76_RS13765 at 2870.101 kb on + strand, within BWI76_RS13765 at 2870.288 kb on - strand, within BWI76_RS13765 at 2870.288 kb on - strand, within BWI76_RS13765
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 2,868,041 + BWI76_RS13745 0.73 +0.8 2,868,076 + BWI76_RS13745 0.77 -2.2 2,868,094 + BWI76_RS13745 0.79 +0.6 2,868,114 - BWI76_RS13745 0.81 -0.8 2,868,358 - BWI76_RS13750 0.13 +1.1 2,868,403 + BWI76_RS13750 0.20 +0.1 2,868,411 - BWI76_RS13750 0.21 +0.5 2,868,556 - BWI76_RS13750 0.43 +0.2 2,868,573 + BWI76_RS13750 0.46 +1.4 2,868,573 + BWI76_RS13750 0.46 +0.4 2,868,573 + BWI76_RS13750 0.46 +0.9 2,868,654 + BWI76_RS13750 0.58 +0.2 2,868,711 + BWI76_RS13750 0.67 -0.0 2,868,719 + BWI76_RS13750 0.68 +0.2 2,868,719 + BWI76_RS13750 0.68 +0.9 2,868,824 - BWI76_RS13750 0.84 -2.6 2,868,827 + BWI76_RS13750 0.84 -0.2 2,868,827 + BWI76_RS13750 0.84 +0.4 2,868,830 - BWI76_RS13750 0.85 +2.1 2,868,865 - -0.8 2,868,883 + -0.4 2,868,886 - +0.5 2,868,931 + -0.4 2,868,960 + +0.2 2,868,968 - -0.6 2,868,970 + -1.8 2,868,978 - -1.9 2,869,189 - BWI76_RS13755 0.67 -3.2 2,869,191 + BWI76_RS13755 0.68 -1.4 2,869,264 + +1.1 2,869,265 + +0.8 2,869,293 + +0.8 2,869,303 + -0.4 2,869,310 + +0.2 2,869,310 - +1.1 2,869,387 - BWI76_RS13760 0.49 +0.2 2,869,392 - -1.2 2,869,393 + BWI76_RS13760 0.52 -2.2 2,869,435 - -0.2 2,869,460 + BWI76_RS13760 0.87 -1.6 2,869,468 - -0.7 2,869,503 - +0.8 2,869,549 + -0.5 2,869,549 + +0.8 2,869,549 + +0.8 2,869,554 + +0.8 2,869,557 - -0.2 2,869,562 - -0.6 2,869,581 - +1.9 2,869,656 + -2.0 2,869,661 + -0.8 2,869,663 + +0.9 2,869,664 - +1.5 2,869,666 + +2.1 2,869,669 - -0.2 2,869,669 - -1.2 2,869,679 - -1.2 2,869,679 - -0.3 2,869,993 + BWI76_RS13765 0.13 -0.8 2,870,009 + BWI76_RS13765 0.15 -1.6 2,870,014 + BWI76_RS13765 0.15 -0.2 2,870,101 + BWI76_RS13765 0.23 -0.2 2,870,288 - BWI76_RS13765 0.39 +0.2 2,870,288 - BWI76_RS13765 0.39 +0.1
Or see this region's nucleotide sequence